Q05085 · PTR7_ARATH
- ProteinProtein NRT1/ PTR FAMILY 6.3
- GeneNPF6.3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids590 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Dual affinity nitrate transporter. Involved in proton-dependent nitrate uptake and in the regulation of the nitrate transporter NRT2.1. Acts also as a nitrate sensor that trigger a specific signaling pathway stimulating lateral root growth and seed germination. The uptake activity is not required for sensor function. Displays an auxin transport facilitation inhibited by high nitrate concentration. Required to prevent auxin accumulation in preemerged lateral root primordia and young lateral roots when external nitrate concentration is low or null. May be involved in the basipetal transport of auxin out of the lateral root tips. Acts as a bidirectional transporter involved in root-to-shoot nitrate translocation. Recognizes specifically nitrate and chlorate, but not nitrite, alanine, sulfate, phosphate or the di-peptide Ala-Ala.
Miscellaneous
When mutated confers resistance to the herbicide chlorate.
The kinase CIPK23 is a negative regulator of the high-affinity response, while the kinase CIPK8 is a positive regulator of the low-affinity response. Thr-101 is not the direct target of CIPK8.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
49 μM | nitrate | 5.5 | for the high-affinity phase, in the presence of 250 uM nitrate | |||
4 mM | nitrate | 5.5 | for the low-affinity phase, in the presence of 10 mM nitrate |
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 356 | substrate | ||||
Sequence: H | ||||||
Binding site | 360 | substrate | ||||
Sequence: T |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | nitrate transmembrane transporter activity | |
Molecular Function | symporter activity | |
Biological Process | auxin-activated signaling pathway | |
Biological Process | basipetal auxin transport | |
Biological Process | lateral root development | |
Biological Process | nitrate assimilation | |
Biological Process | nitrate transmembrane transport | |
Biological Process | oligopeptide transport | |
Biological Process | photoperiodism, flowering | |
Biological Process | response to herbicide | |
Biological Process | response to nitrate | |
Biological Process | response to water deprivation |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameProtein NRT1/ PTR FAMILY 6.3
- Short namesAtNPF6.3
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ05085
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 46-66 | Helical | ||||
Sequence: LTTLGIGVNLVTYLTGTMHLG | ||||||
Transmembrane | 77-97 | Helical | ||||
Sequence: FLGTSFMLCLLGGFIADTFLG | ||||||
Transmembrane | 102-122 | Helical | ||||
Sequence: IAIFAAIQATGVSILTLSTII | ||||||
Transmembrane | 143-163 | Helical | ||||
Sequence: GIQLTVLYLALYLTALGTGGV | ||||||
Transmembrane | 193-213 | Helical | ||||
Sequence: FFFCINVGSLLAVTVLVYVQD | ||||||
Transmembrane | 219-239 | Helical | ||||
Sequence: WGYGICAFAIVLALSVFLAGT | ||||||
Transmembrane | 342-362 | Helical | ||||
Sequence: MLPIWATCILFWTVHAQLTTL | ||||||
Transmembrane | 374-394 | Helical | ||||
Sequence: IGSFEIPPASMAVFYVGGLLL | ||||||
Transmembrane | 423-443 | Helical | ||||
Sequence: IGLGLFFGSMAMAVAALVELK | ||||||
Transmembrane | 460-480 | Helical | ||||
Sequence: LGFYLLIPQYLIVGIGEALIY | ||||||
Transmembrane | 501-521 | Helical | ||||
Sequence: GLLLSTLALGFFFSSVLVTIV | ||||||
Transmembrane | 542-562 | Helical | ||||
Sequence: YNFYWLVAVLVALNFLIFLVF |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Altered development of nascent organs. Reduced stomatal opening and reduced transpiration rates in the light resulting in enhanced drought tolerance. Slower translocation of nitrate to the leaves.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 28 | No effect on phosphorylation and on nitrate transport. | ||||
Sequence: T → A | ||||||
Mutagenesis | 41 | Loss of the transporter activity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 44 | Loss of the transporter activity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 45 | Loss of the transporter activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 101 | Loss of phosphorylation and 91% reduction of high-affinity nitrate transport, but no effect on the nitrate binding. | ||||
Sequence: T → A | ||||||
Mutagenesis | 101 | Loss of low-affinity nitrate transport. | ||||
Sequence: T → D | ||||||
Mutagenesis | 130 | 90% reduction of the transporter activity. | ||||
Sequence: C → A | ||||||
Mutagenesis | 164 | 90% reduction of the transporter activity. | ||||
Sequence: K → A | ||||||
Mutagenesis | 356 | Loss of the transporter activity. | ||||
Sequence: H → A | ||||||
Mutagenesis | 476 | 80% reduction of the transporter activity. | ||||
Sequence: E → A | ||||||
Mutagenesis | 492 | In chl1-9; loss of high- and low-affinity nitrate transport, but no effect on nitrate sensing. | ||||
Sequence: P → L |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000064316 | 1-590 | Protein NRT1/ PTR FAMILY 6.3 | |||
Sequence: MSLPETKSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPTTSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYWLVAVLVALNFLIFLVFSKWYVYKEKRLAEVGIELDDEPSIPMGH | ||||||
Modified residue | 101 | Phosphothreonine; by CIPK23 | ||||
Sequence: T |
Post-translational modification
Acts as a high-affinity nitrate transporter when phosphorylated and as a low-affinity transporter when dephosphorylated. Forms homodimer when unphosphorylated and monomer when phosphorylated. Low nitrogen concentration in the medium stimulates phosphorylation. Phosphorylation also regulates the nitrate signaling.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the stele in lateral root primordia before emergence and in the tip of primary and emerged lateral roots. Detected in emerging and immature leaves, guard cells, flower buds, style, stigma, anthers and pollen grains. Not detected in the shoot apical meristem.
Induction
By nitrate and auxin.
Developmental stage
Expressed in the columella root cap at day 1 after germination. At day 3, detected in the root meristematic region and at day 5, expressed throughout the root tip.
Gene expression databases
Interaction
Subunit
Monomer and homodimer. The dimer has the 2 monomers in the same orientation. Interacts with CIPK23.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q05085 | CIPK23 Q93VD3 | 3 | EBI-2463703, EBI-974277 |
Protein-protein interaction databases
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length590
- Mass (Da)64,922
- Last updated1994-06-01 v1
- Checksum78D3E660AAF90D47
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L10357 EMBL· GenBank· DDBJ | AAA32770.1 EMBL· GenBank· DDBJ | mRNA | ||
AC002131 EMBL· GenBank· DDBJ | AAC17604.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002684 EMBL· GenBank· DDBJ | AEE28838.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BT002016 EMBL· GenBank· DDBJ | AAN72027.1 EMBL· GenBank· DDBJ | mRNA | ||
BT008783 EMBL· GenBank· DDBJ | AAP68222.1 EMBL· GenBank· DDBJ | mRNA | ||
AK317498 EMBL· GenBank· DDBJ | BAH20163.1 EMBL· GenBank· DDBJ | mRNA |