Q05085 · PTR7_ARATH

Function

function

Dual affinity nitrate transporter. Involved in proton-dependent nitrate uptake and in the regulation of the nitrate transporter NRT2.1. Acts also as a nitrate sensor that trigger a specific signaling pathway stimulating lateral root growth and seed germination. The uptake activity is not required for sensor function. Displays an auxin transport facilitation inhibited by high nitrate concentration. Required to prevent auxin accumulation in preemerged lateral root primordia and young lateral roots when external nitrate concentration is low or null. May be involved in the basipetal transport of auxin out of the lateral root tips. Acts as a bidirectional transporter involved in root-to-shoot nitrate translocation. Recognizes specifically nitrate and chlorate, but not nitrite, alanine, sulfate, phosphate or the di-peptide Ala-Ala.

Miscellaneous

When mutated confers resistance to the herbicide chlorate.
The kinase CIPK23 is a negative regulator of the high-affinity response, while the kinase CIPK8 is a positive regulator of the low-affinity response. Thr-101 is not the direct target of CIPK8.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
49 μMnitrate5.5for the high-affinity phase, in the presence of 250 uM nitrate
4 mMnitrate5.5for the low-affinity phase, in the presence of 10 mM nitrate

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site356substrate
Binding site360substrate

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Functionnitrate transmembrane transporter activity
Molecular Functionsymporter activity
Biological Processauxin-activated signaling pathway
Biological Processbasipetal auxin transport
Biological Processlateral root development
Biological Processnitrate assimilation
Biological Processnitrate transmembrane transport
Biological Processoligopeptide transport
Biological Processphotoperiodism, flowering
Biological Processresponse to herbicide
Biological Processresponse to nitrate
Biological Processresponse to water deprivation

Keywords

Enzyme and pathway databases

Protein family/group databases

    • 2.A.17.3.1the proton-dependent oligopeptide transporter (pot/ptr) family

Names & Taxonomy

Protein names

  • Recommended name
    Protein NRT1/ PTR FAMILY 6.3
  • Short names
    AtNPF6.3
  • Alternative names
    • Nitrate transporter 1.1 (AtNRT1)
    • Nitrate/chlorate transporter
    • Protein CHLORINA 1

Gene names

    • Name
      NPF6.3
    • Synonyms
      CHL1, NRT1, NRT1.1
    • ORF names
      F12F1.1, T28K15_13
    • Ordered locus names
      At1g12110

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q05085
  • Secondary accessions
    • B9DHE6

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane46-66Helical
Transmembrane77-97Helical
Transmembrane102-122Helical
Transmembrane143-163Helical
Transmembrane193-213Helical
Transmembrane219-239Helical
Transmembrane342-362Helical
Transmembrane374-394Helical
Transmembrane423-443Helical
Transmembrane460-480Helical
Transmembrane501-521Helical
Transmembrane542-562Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Altered development of nascent organs. Reduced stomatal opening and reduced transpiration rates in the light resulting in enhanced drought tolerance. Slower translocation of nitrate to the leaves.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis28No effect on phosphorylation and on nitrate transport.
Mutagenesis41Loss of the transporter activity.
Mutagenesis44Loss of the transporter activity.
Mutagenesis45Loss of the transporter activity.
Mutagenesis101Loss of phosphorylation and 91% reduction of high-affinity nitrate transport, but no effect on the nitrate binding.
Mutagenesis101Loss of low-affinity nitrate transport.
Mutagenesis13090% reduction of the transporter activity.
Mutagenesis16490% reduction of the transporter activity.
Mutagenesis356Loss of the transporter activity.
Mutagenesis47680% reduction of the transporter activity.
Mutagenesis492In chl1-9; loss of high- and low-affinity nitrate transport, but no effect on nitrate sensing.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000643161-590Protein NRT1/ PTR FAMILY 6.3
Modified residue101Phosphothreonine; by CIPK23

Post-translational modification

Acts as a high-affinity nitrate transporter when phosphorylated and as a low-affinity transporter when dephosphorylated. Forms homodimer when unphosphorylated and monomer when phosphorylated. Low nitrogen concentration in the medium stimulates phosphorylation. Phosphorylation also regulates the nitrate signaling.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in the stele in lateral root primordia before emergence and in the tip of primary and emerged lateral roots. Detected in emerging and immature leaves, guard cells, flower buds, style, stigma, anthers and pollen grains. Not detected in the shoot apical meristem.

Induction

By nitrate and auxin.

Developmental stage

Expressed in the columella root cap at day 1 after germination. At day 3, detected in the root meristematic region and at day 5, expressed throughout the root tip.

Gene expression databases

Interaction

Subunit

Monomer and homodimer. The dimer has the 2 monomers in the same orientation. Interacts with CIPK23.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q05085CIPK23 Q93VD33EBI-2463703, EBI-974277

Protein-protein interaction databases

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    590
  • Mass (Da)
    64,922
  • Last updated
    1994-06-01 v1
  • Checksum
    78D3E660AAF90D47
MSLPETKSDDILLDAWDFQGRPADRSKTGGWASAAMILCIEAVERLTTLGIGVNLVTYLTGTMHLGNATAANTVTNFLGTSFMLCLLGGFIADTFLGRYLTIAIFAAIQATGVSILTLSTIIPGLRPPRCNPTTSSHCEQASGIQLTVLYLALYLTALGTGGVKASVSGFGSDQFDETEPKERSKMTYFFNRFFFCINVGSLLAVTVLVYVQDDVGRKWGYGICAFAIVLALSVFLAGTNRYRFKKLIGSPMTQVAAVIVAAWRNRKLELPADPSYLYDVDDIIAAEGSMKGKQKLPHTEQFRSLDKAAIRDQEAGVTSNVFNKWTLSTLTDVEEVKQIVRMLPIWATCILFWTVHAQLTTLSVAQSETLDRSIGSFEIPPASMAVFYVGGLLLTTAVYDRVAIRLCKKLFNYPHGLRPLQRIGLGLFFGSMAMAVAALVELKRLRTAHAHGPTVKTLPLGFYLLIPQYLIVGIGEALIYTGQLDFFLRECPKGMKGMSTGLLLSTLALGFFFSSVLVTIVEKFTGKAHPWIADDLNKGRLYNFYWLVAVLVALNFLIFLVFSKWYVYKEKRLAEVGIELDDEPSIPMGH

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
L10357
EMBL· GenBank· DDBJ
AAA32770.1
EMBL· GenBank· DDBJ
mRNA
AC002131
EMBL· GenBank· DDBJ
AAC17604.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002684
EMBL· GenBank· DDBJ
AEE28838.1
EMBL· GenBank· DDBJ
Genomic DNA
BT002016
EMBL· GenBank· DDBJ
AAN72027.1
EMBL· GenBank· DDBJ
mRNA
BT008783
EMBL· GenBank· DDBJ
AAP68222.1
EMBL· GenBank· DDBJ
mRNA
AK317498
EMBL· GenBank· DDBJ
BAH20163.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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