Q05016 · YM71_YEAST
- ProteinNADP-dependent 3-hydroxy acid dehydrogenase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids267 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
NADP-dependent dehydrogenase with broad substrate specificity acting on 3-hydroxy acids. Catalyzes the NADP-dependent oxidation of L-allo-threonine to L-2-amino-3-keto-butyrate, which is spontaneously decarboxylated into aminoacetone. Also acts on D-threonine, L-serine, D-serine, D-3-hydroxyisobutyrate, L-3-hydroxyisobutyrate, D-glycerate and L-glycerate.
Miscellaneous
Present with 3210 molecules/cell in log phase SD medium.
Catalytic activity
- L-allo-threonine + NADP+ = aminoacetone + CO2 + NADPH
- L-allo-threonine + NADP+ = (2S)-2-amino-3-oxobutanoate + H+ + NADPH
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.5 mM | NADP+ | 9 | 30 | |||
95 mM | L-serine | 9 | 30 | |||
71 mM | D-serine | 9 | 30 | |||
7 mM | D-threonine | 9 | 30 | |||
3 mM | L-allo-threonine | 9 | 30 | |||
36 mM | L-3-hydroxyisobutyrate | 9 | 30 | |||
57 mM | D-3-hydroxyisobutyrate | 9 | 30 | |||
95 mM | L-3-hydroxybutyrate | 9 | 30 |
The highest catalytic efficiency was observed with L-allo-threonine.
pH Dependence
Optimum pH is about 8.5 for the oxidation of L-serine. Stable from pH 7.5 to 10.5.
Temperature Dependence
Stable up to 40 degrees Celsius.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 18 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 75 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 102 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Active site | 168 | Proton acceptor | ||||
Sequence: Y | ||||||
Active site | 168 | Proton donor | ||||
Sequence: Y | ||||||
Binding site | 168 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Active site | 172 | Lowers pKa of active site Tyr | ||||
Sequence: K | ||||||
Binding site | 172 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 201 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: V |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | carbonyl reductase (NADPH) activity | |
Molecular Function | diacetyl reductase ((S)-acetoin forming) activity | |
Molecular Function | oxidoreductase activity | |
Molecular Function | serine 3-dehydrogenase activity | |
Biological Process | acetoin metabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADP-dependent 3-hydroxy acid dehydrogenase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ05016
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000054873 | 1-267 | NADP-dependent 3-hydroxy acid dehydrogenase | |||
Sequence: MSQGRKAAERLAKKTVLITGASAGIGKATALEYLEASNGDMKLILAARRLEKLEELKKTIDQEFPNAKVHVAQLDITQAEKIKPFIENLPQEFKDIDILVNNAGKALGSDRVGQIATEDIQDVFDTNVTALINITQAVLPIFQAKNSGDIVNLGSIAGRDAYPTGSIYCASKFAVGAFTDSLRKELINTKIRVILIAPGLVETEFSLVRYRGNEEQAKNVYKDTTPLMADDVADLIVYATSRKQNTVIADTLIFPTNQASPHHIFRG | ||||||
Modified residue | 260 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Homotetramer.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q05016 | DUN1 P39009 | 2 | EBI-27486, EBI-6194 | |
BINARY | Q05016 | SSA2 P10592 | 2 | EBI-27486, EBI-8603 |
Protein-protein interaction databases
Miscellaneous
Structure
Sequence
- Sequence statusComplete
- Length267
- Mass (Da)29,158
- Last updated1997-11-01 v1
- Checksum77FCD713F724A9D7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z49939 EMBL· GenBank· DDBJ | CAA90197.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006946 EMBL· GenBank· DDBJ | DAA10125.1 EMBL· GenBank· DDBJ | Genomic DNA |