Q04ZG6 · CARA_LEPBL

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site45L-glutamine (UniProtKB | ChEBI)
Binding site224L-glutamine (UniProtKB | ChEBI)
Binding site226L-glutamine (UniProtKB | ChEBI)
Active site252Nucleophile
Binding site253L-glutamine (UniProtKB | ChEBI)
Binding site256L-glutamine (UniProtKB | ChEBI)
Binding site294L-glutamine (UniProtKB | ChEBI)
Binding site296L-glutamine (UniProtKB | ChEBI)
Binding site297L-glutamine (UniProtKB | ChEBI)
Active site335
Active site337

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • Ordered locus names
      LBL_2116

Organism names

Accessions

  • Primary accession
    Q04ZG6

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10001388651-363Carbamoyl phosphate synthase small chain

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-172CPSase
Domain176-362Glutamine amidotransferase type-1

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    363
  • Mass (Da)
    40,053
  • Last updated
    2006-11-14 v1
  • Checksum
    7FF0D5ACCBC09CD4
MKAFLVLDNGMILEGESFGYESESVGEIVFNTSMAGYQEILTDPSYCNQIITLTYPMIGNYGIHPDNMESSKIQASGLIVREYVDLPSNFMSQKTLSKFLKEYQIPAIQGIDTRKLTRYIRTNGSPNGGIFVAREYSPEFLEKVKSFPGITGTDLAKVVTTSTKYIFGTHTGKKYKLAVYDYGVKTNILRLLDAAGFAVTVYPALTPAEEIMKEGTDAFFLSNGPGDPAPLDYAIDATRKIMEKGYPLFGICLGHQIIGLSLGKKTEKMKFGHRGGNQPVKNLSTGQVEITSQNHGFAVIDDGKSSEPVSFLNLNDNTVEGILKSGYPLLTVQYHPESAPGPNDSRYLFQKFYDLVETTKRGK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000348
EMBL· GenBank· DDBJ
ABJ79529.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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