Q04Y85 · MURE_LEPBL

Function

function

Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site38UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate (UniProtKB | ChEBI)
Binding site118-124ATP (UniProtKB | ChEBI)
Binding site160-161UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate (UniProtKB | ChEBI)
Binding site187UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate (UniProtKB | ChEBI)
Binding site195UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate (UniProtKB | ChEBI)
Binding site395meso-2,6-diaminoheptanedioate (UniProtKB | ChEBI)
Binding site419-422meso-2,6-diaminoheptanedioate (UniProtKB | ChEBI)
Binding site471meso-2,6-diaminoheptanedioate (UniProtKB | ChEBI)
Binding site475meso-2,6-diaminoheptanedioate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionmagnesium ion binding
Molecular FunctionUDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity
Biological Processcell division
Biological Processcell wall organization
Biological Processpeptidoglycan biosynthetic process
Biological Processregulation of cell shape

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
  • EC number
  • Alternative names
    • Meso-A2pm-adding enzyme
    • Meso-diaminopimelate-adding enzyme
    • UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    • UDP-MurNAc-tripeptide synthetase
    • UDP-N-acetylmuramyl-tripeptide synthetase

Gene names

    • Name
      murE
    • Ordered locus names
      LBL_2600

Organism names

Accessions

  • Primary accession
    Q04Y85

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_10000123711-500UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
Modified residue227N6-carboxylysine

Post-translational modification

Carboxylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP.

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif419-422Meso-diaminopimelate recognition motif

Sequence similarities

Belongs to the MurCDEF family. MurE subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    500
  • Mass (Da)
    54,801
  • Last updated
    2006-11-14 v1
  • Checksum
    7956C64A14928D2C
MKMKLTNLLHEFPELKLGSLPSGKNPDSVPIEYIQSDSRKTNPEDIFCVPESIGTKKGEFISNTKASVILLHRSSNISIDSSKIVLECEEDPEQLQGRIASFLLGHPSKTLEIVAVTGTNGKTSLTNILFALAKDQGKICGLIGTIGVKFGDRSIDTGYTTPDASSLNLILKQMKDEGVTTVFMEASSHGLKLGRIGGISLKAGVFTNLTQDHLDFHSDMEDYFESKFRLFEILDVSKSPFAVLDYSSPGGNELHRKIRNNLPDLPIKALDGIGGEYKVSNPFLTLQGTSYVLSLPGNRSQTISTNLLGSFNVRNTALAFLTGLGLGLDPKGMFSSLKEIPQIPGRFQIVYSKDRSRMAVVDYAHTPDALENIIRSVRNSRPKRLITLFGCGGDRDKTKRPKMARIAEELSDQVILTSDNPRSEKPEAILDEIQSGFSPGFTPLLREVDRARAISEGVGILPEGGCLLVAGKGHEEYQIIGKEKRHFSDVEEVRKAFGLF

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000348
EMBL· GenBank· DDBJ
ABJ79960.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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