Q04UA3 · MIAB_LEPBJ

Function

function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site20[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site56[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site90[4Fe-4S] cluster 1 (UniProtKB | ChEBI)
Binding site164[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site168[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site171[4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular Functionmethylthiotransferase activity
Biological ProcesstRNA methylthiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
  • EC number
  • Alternative names
    • (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
    • tRNA-i(6)A37 methylthiotransferase

Gene names

    • Name
      miaB
    • Ordered locus names
      LBJ_0869

Organism names

Accessions

  • Primary accession
    Q04UA3

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003743601-449tRNA-2-methylthio-N6-dimethylallyladenosine synthase

Interaction

Subunit

Monomer.

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain11-127MTTase N-terminal
Domain150-378Radical SAM core
Domain381-449TRAM

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    449
  • Mass (Da)
    50,906
  • Last updated
    2006-11-14 v1
  • Checksum
    7D4E4D80C4913BB6
MGVLEREKKTGKVYIETYGCQMNEYDSGIVSSLMKDAEYSSSPDPENSDIIFLNTCAIRENAHAKIYNRLQSLGYLKKRNPELVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIRSGEHSISLTRLSKIETYDEIEPRVVNGIQAFVTIMRGCNNFCTFCVVPYTRGRERSRDPKSIVREIQDLTEKGIRQVTLLGQNVNSYKEQGTDFAGLIQMLLDETTIERIRFTSPHPKDFPVRLLRLMAQNPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVREIRNIVPDVGITTDIIVGFPNETEEEFEDTLAVVREVQFDMAFMFKYSEREGTMARKKLPDNVSEETKSARLTKLVDLQTSISHEQNRARIGRVYSILIENTSRKSEKQLCGRTPCGRMTVFPLPEGRSASEMIGSTVSVRIESATSATLKGGILS

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000350
EMBL· GenBank· DDBJ
ABJ75517.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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