Q04809 · DPAA_BACSU
- ProteinDipicolinate synthase subunit A
- GenedpaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids297 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Together with DpaB, catalyzes the conversion of dihydrodipicolinate to dipicolinate (DPA), which constitutes up to 10% of the dry weight of the spore.
Catalytic activity
- (S)-2,3-dihydrodipicolinate + NADP+ = dipicolinate + NADPH + H+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 164-165 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: RT | ||||||
Binding site | 185 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 203 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 242-244 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: LAS | ||||||
Binding site | 264-267 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: APGL |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | oxidoreductase activity | |
Biological Process | sporulation resulting in formation of a cellular spore |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDipicolinate synthase subunit A
- EC number
- Short namesDPA synthase subunit A
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionQ04809
Proteomes
Phenotypes & Variants
Disruption phenotype
Disruption of this gene has no effect on vegetative growth but causes a sporulation defect, characterized by heat-sensitive spores devoid of DPA, which can be cured by supplementation with dipicolinate.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000072083 | 1-297 | Dipicolinate synthase subunit A | |||
Sequence: MLTGLKIAVIGGDARQLEIIRKLTEQQADIYLVGFDQLDHGFTGAVKCNIDEIPFQQIDSIILPVSATTGEGVVSTVFSNEEVVLKQDHLDRTPAHCVIFSGISNAYLENIAAQAKRKLVKLFERDDIAIYNSIPTVEGTIMLAIQHTDYTIHGSQVAVLGLGRTGMTIARTFAALGANVKVGARSSAHLARITEMGLVPFHTDELKEHVKDIDICINTIPSMILNQTVLSSMTPKTLILDLASRPGGTDFKYAEKQGIKALLAPGLPGIVAPKTAGQILANVLSKLLAEIQAEEGK |
Proteomic databases
Expression
Induction
Is not expressed during vegetative growth; is expressed at stage 5 of sporulation specifically in the mother cell compartment, under the control of the sigma-K factor. Is repressed by GerE.
Interaction
Subunit
Dipicolinate synthase likely consists of DpaA and DpaB, since both proteins are required for DPA synthesis.
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length297
- Mass (Da)31,948
- Last updated1993-10-01 v1
- Checksum634B14D514B041B9
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L08471 EMBL· GenBank· DDBJ | AAA22381.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z22554 EMBL· GenBank· DDBJ | CAA80274.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL009126 EMBL· GenBank· DDBJ | CAB13546.1 EMBL· GenBank· DDBJ | Genomic DNA |