Q046D1 · RPOC_LACGA
- ProteinDNA-directed RNA polymerase subunit beta'
- GenerpoC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids1223 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Catalytic activity
- RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Mg2+ ion per subunit.
Note: Binds 2 Zn2+ ions per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 60 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 62 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 75 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 78 | Zn2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 449 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 451 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 453 | Mg2+ (UniProtKB | ChEBI) | |||
Binding site | 818 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 892 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 899 | Zn2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 902 | Zn2+ 2 (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | DNA-directed RNA polymerase complex | |
Molecular Function | DNA binding | |
Molecular Function | DNA-directed 5'-3' RNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA-directed RNA polymerase subunit beta'
- EC number
- Short namesRNAP subunit beta'
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Lactobacillus
Accessions
- Primary accessionQ046D1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000308843 | 1-1223 | DNA-directed RNA polymerase subunit beta' | ||
Interaction
Subunit
The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
Structure
Sequence
- Sequence statusComplete
- Length1,223
- Mass (Da)136,371
- Last updated2006-11-14 v1
- Checksum8956074C0A19132C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000413 EMBL· GenBank· DDBJ | ABJ59691.1 EMBL· GenBank· DDBJ | Genomic DNA |