Q04373 · PUF6_YEAST
- ProteinPumilio homology domain family member 6
- GenePUF6
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids656 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
RNA-binding protein involved in post-transcriptional regulation. Component of the ASH1 mRNP which transports the ASH1 mRNA to the distal tip of the bud, where the ASH1 protein is translated and targeted to the daughter cell nucleus. Binds to the ASH1 3'-UTR containing the PUF consensus UUGU segment and represses its translation. This silencing of ASH1 mRNA is critical for asymmetric seggregation of ASH1 to the daughter cell nucleus.
Miscellaneous
Present with 21500 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cellular bud tip | |
Cellular Component | large ribosomal subunit | |
Cellular Component | nucleolus | |
Cellular Component | nucleus | |
Cellular Component | preribosome, large subunit precursor | |
Cellular Component | protein folding chaperone complex | |
Molecular Function | large ribosomal subunit rRNA binding | |
Molecular Function | mRNA 3'-UTR binding | |
Molecular Function | mRNA 5'-UTR binding | |
Molecular Function | mRNA binding | |
Molecular Function | mRNA regulatory element binding translation repressor activity | |
Biological Process | negative regulation of translation | |
Biological Process | protein stabilization | |
Biological Process | regulation of translation | |
Biological Process | ribosomal large subunit biogenesis |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePumilio homology domain family member 6
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ04373
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 31 | Increases translation repression of ASH1 mRNA. | |||
Mutagenesis | 34 | Increases translation repression of ASH1 mRNA. | |||
Mutagenesis | 35 | Increases translation repression of ASH1 mRNA. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 17 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000075926 | 1-656 | Pumilio homology domain family member 6 | ||
Modified residue | 31 | Phosphoserine; by CK2 | |||
Modified residue | 34 | Phosphoserine | |||
Modified residue | 34 | Phosphoserine; by CK2 | |||
Modified residue | 35 | Phosphoserine | |||
Modified residue | 35 | Phosphoserine; by CK2 | |||
Post-translational modification
phosphorylation by CK2 relieves translational repression activity.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Component of the ASH1 mRNP composed of at least PUF6, SHE2, SHE3, SHE1 and the ASH1 mRNA. Interacts with SHE2 and FUN12.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q04373 | SHE2 P36068 | 2 | EBI-33208, EBI-26866 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, repeat.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-107 | Disordered | |||
Compositional bias | 9-50 | Basic and acidic residues | |||
Compositional bias | 51-85 | Acidic residues | |||
Compositional bias | 86-107 | Basic and acidic residues | |||
Domain | 133-483 | PUM-HD | |||
Repeat | 155-191 | Pumilio 1 | |||
Repeat | 192-227 | Pumilio 2 | |||
Repeat | 228-264 | Pumilio 3 | |||
Repeat | 340-376 | Pumilio 4 | |||
Repeat | 377-413 | Pumilio 5 | |||
Repeat | 415-450 | Pumilio 6 | |||
Sequence similarities
Belongs to the PUF6 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length656
- Mass (Da)75,106
- Last updated1996-11-01 v1
- MD5 ChecksumFAC17CA68E6035A88BFF2EAAAC2066D0
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 9-50 | Basic and acidic residues | |||
Compositional bias | 51-85 | Acidic residues | |||
Compositional bias | 86-107 | Basic and acidic residues | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U33057 EMBL· GenBank· DDBJ | AAB64938.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006938 EMBL· GenBank· DDBJ | DAA12328.1 EMBL· GenBank· DDBJ | Genomic DNA |