Essential maintenance is planned to begin on Fri Jan 24 2025. The website may be temporarily unavailable. Please use our fallback: https://wwwdev.ebi.ac.uk/uniprot/front-end/fallback/ in case of any outage.

Q04373 · PUF6_YEAST

Function

function

RNA-binding protein involved in post-transcriptional regulation. Component of the ASH1 mRNP which transports the ASH1 mRNA to the distal tip of the bud, where the ASH1 protein is translated and targeted to the daughter cell nucleus. Binds to the ASH1 3'-UTR containing the PUF consensus UUGU segment and represses its translation. This silencing of ASH1 mRNA is critical for asymmetric seggregation of ASH1 to the daughter cell nucleus.

Miscellaneous

Present with 21500 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcellular bud tip
Cellular Componentlarge ribosomal subunit
Cellular Componentnucleolus
Cellular Componentnucleus
Cellular Componentpreribosome, large subunit precursor
Cellular Componentprotein folding chaperone complex
Molecular Functionlarge ribosomal subunit rRNA binding
Molecular FunctionmRNA 3'-UTR binding
Molecular FunctionmRNA 5'-UTR binding
Molecular FunctionmRNA binding
Molecular FunctionmRNA regulatory element binding translation repressor activity
Biological Processnegative regulation of translation
Biological Processprotein stabilization
Biological Processregulation of translation
Biological Processribosomal large subunit biogenesis

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Pumilio homology domain family member 6

Gene names

    • Name
      PUF6
    • ORF names
      D9719.2
    • Ordered locus names
      YDR496C

Organism names

Accessions

  • Primary accession
    Q04373
  • Secondary accessions
    • D6VTB8

Proteomes

Organism-specific databases

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis31Increases translation repression of ASH1 mRNA.
Mutagenesis34Increases translation repression of ASH1 mRNA.
Mutagenesis35Increases translation repression of ASH1 mRNA.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 17 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain, modified residue.

Type
IDPosition(s)Description
ChainPRO_00000759261-656Pumilio homology domain family member 6
Modified residue31Phosphoserine; by CK2
Modified residue34Phosphoserine
Modified residue34Phosphoserine; by CK2
Modified residue35Phosphoserine
Modified residue35Phosphoserine; by CK2

Post-translational modification

phosphorylation by CK2 relieves translational repression activity.

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Component of the ASH1 mRNP composed of at least PUF6, SHE2, SHE3, SHE1 and the ASH1 mRNA. Interacts with SHE2 and FUN12.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntAct
BINARY Q04373SHE2 P360682EBI-33208, EBI-26866

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for region, compositional bias, domain, repeat.

Type
IDPosition(s)Description
Region1-107Disordered
Compositional bias9-50Basic and acidic residues
Compositional bias51-85Acidic residues
Compositional bias86-107Basic and acidic residues
Domain133-483PUM-HD
Repeat155-191Pumilio 1
Repeat192-227Pumilio 2
Repeat228-264Pumilio 3
Repeat340-376Pumilio 4
Repeat377-413Pumilio 5
Repeat415-450Pumilio 6

Sequence similarities

Belongs to the PUF6 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    656
  • Mass (Da)
    75,106
  • Last updated
    1996-11-01 v1
  • MD5 Checksum
    FAC17CA68E6035A88BFF2EAAAC2066D0
MAPLTKKTNGKRSAKEVSHSEKKLAKKPRISIDSSDEESELSKKEDAVSSSSDDDDLDDLSTSDSEAEEEADELDISDDSEEHENENEEKEGKDKSEGGENGNHTEQRKLLKERKMQRKSGTQVQQIKSVWERLRVKTPPLPKQIREKLSNEIWELSKDCISDLVLKHDASRIVQTLVKYSSKDRREQIVDALKGKFYVLATSAYGKYLLVKLLHYGSRSSRQTIINELHGSLRKLMRHREGAYVVEDLFVLYATHEQRQQMIKEFWGSEYAVFRETHKDLTIEKVCESSIEKRNIIARNLIGTITASVEKGSTGFQILHAAMREYVKIANEKEISEMIELLHEQFAELVHTPEGSDVACTLVARANAKERKLILKALKNHAEKLIKNEYGNIVFITILNCVDDTVLVFKTFSPTVKEHLQEFIIDKFGRRPWLYILLGLDGKYFSPIVKNELLRYIELSKATSKKDPLQRRHELLSKFAPMFLSTISKDYSSILTENLGCQFIAEVLINDELYAQLNEKDQEKYQQVLNNILTTFKGDITEEEHPIHRAFSTRLLKALIQGGKWNNKEKKVIPLKNVQGLGVPFAEKLYDEIIDSSNLLEWINNADSSFTIVALYETLKDQKEGKPFLKDLRGVQSKITTDESNKGSQLLAKLLK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias9-50Basic and acidic residues
Compositional bias51-85Acidic residues
Compositional bias86-107Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U33057
EMBL· GenBank· DDBJ
AAB64938.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006938
EMBL· GenBank· DDBJ
DAA12328.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help