Q04372 · TAP42_YEAST
- ProteinType 2A phosphatase-associated protein 42
- GeneTAP42
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids366 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in negative regulation of the TOR signaling pathway in response to type of available nitrogen source. Inhibitor of PP2A phosphatase SIT4, which results in inhibition of nuclear export of MSN2, due to lack of dephosphorylation by SIT4. Also required for rapamycin induced activation of expression of many nitrogen discrimination pathway (NDP) genes. In complex with PPH21, required for organization of the actin cytoskeletom during the cell cycle via a Rho GTPase-dependent mechanism.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | protein phosphatase type 2A complex | |
Molecular Function | protein phosphatase 2A binding | |
Biological Process | negative regulation of signal transduction | |
Biological Process | positive regulation of transcription by RNA polymerase I | |
Biological Process | regulation of dephosphorylation | |
Biological Process | regulation of TORC1 signaling | |
Biological Process | TOR signaling |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameType 2A phosphatase-associated protein 42
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ04372
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000218623 | 1-366 | Type 2A phosphatase-associated protein 42 | |||
Sequence: MASVTEQFNDIISLYSTKLEHTSLRQDSPEYQGLLLSTIKKLLNLKTAIFDRLALFSTNETIDDVSTASIKFLAVDYYLGLLISRRQSNDSDVAQRQSMKLIYLKKSVESFINFLTLLQDYKLLDPLVGEKLGNFKDRYNPQLSELYAQPKNNKDLSGAQLKRKEKIELFQRNKEISTKLHCLELELKNNDEDHDHDELLRELYLMRLHHFSLDTINNIEQNLFECEMLSNFLKNSVHEVKSSGTQIRKESNDDDSTGFTDKLENINKPLIDKKGQVLRNFTLVDKRQQLQQKVRGYGQYGPTMSVEEFLDKEFEEGRVLQGGEEPEQAPDEENMDWQDRETYKAREWDEFKESHAKGSGNTMNRG |
Post-translational modification
Phosphorylated by TOR kinases. Dephosphorylated by CDC55, TPD3 and SIT4.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Associates with the PP2a (PPH21 and PPH22) and SIT4 protein phosphatase catalytic subunits. Interacts with PPG1, PPH3 and TIP41.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q04372 | PPH21 P23594 | 11 | EBI-18926, EBI-12745 | |
BINARY | Q04372 | PPH22 P23595 | 6 | EBI-18926, EBI-12752 | |
BINARY | Q04372 | SIT4 P20604 | 8 | EBI-18926, EBI-13707 | |
BINARY | Q04372 | TOR2 P32600 | 4 | EBI-18926, EBI-19385 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 318-366 | Disordered | ||||
Sequence: RVLQGGEEPEQAPDEENMDWQDRETYKAREWDEFKESHAKGSGNTMNRG | ||||||
Compositional bias | 333-356 | Basic and acidic residues | ||||
Sequence: ENMDWQDRETYKAREWDEFKESHA |
Sequence similarities
Belongs to the IGBP1/TAP42 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length366
- Mass (Da)42,568
- Last updated1997-11-01 v1
- Checksum16F1E236BEC696DD
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 333-356 | Basic and acidic residues | ||||
Sequence: ENMDWQDRETYKAREWDEFKESHA |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U43890 EMBL· GenBank· DDBJ | AAC49396.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z49211 EMBL· GenBank· DDBJ | CAA89131.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Z49213 EMBL· GenBank· DDBJ | CAA89143.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY558399 EMBL· GenBank· DDBJ | AAS56725.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006946 EMBL· GenBank· DDBJ | DAA09926.1 EMBL· GenBank· DDBJ | Genomic DNA |