Q04228 · UBX2_YEAST

Function

function

Integral endoplasmic reticulum membrane protein that coordinates the assembly of the ER-associated protein degradation (ERAD) machinery at the ER membrane. Mediates binding of CDC48 to the E3 ubiquitin ligases SSM4/DOA10 and HRD1, and to ERAD substrates. Component of the DOA10 ubiquitin ligase complex, which is part of the ERAD-C pathway responsible for the rapid degradation of membrane proteins with misfolded cytoplasmic domains. ERAD-C substrates are ubiquitinated through DOA10 in conjunction with the E2 ubiquitin-conjugating enzymes UBC6 and UBC7-CUE1. Also a component of the HRD1 ubiquitin ligase complex, which is part of the ERAD-L and ERAD-M pathways responsible for the rapid degradation of soluble lumenal and membrane proteins with misfolded lumenal domains (ERAD-L), or ER-membrane proteins with misfolded transmembrane domains (ERAD-M). ERAD-L substrates are ubiquitinated through HRD1 in conjunction with the E2 ubiquitin-conjugating enzymes UBC1 and UBC7-CUE1. Ubiquitinated substrates are then removed to the cytosol via the action of the CDC48-NPL4-UFD1 ATPase complex and targeted to the proteasome.

Miscellaneous

Present with 12600 molecules/cell in log phase SD medium.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular ComponentDoa10p ubiquitin ligase complex
Cellular Componentendoplasmic reticulum
Cellular Componentendoplasmic reticulum membrane
Cellular ComponentHrd1p ubiquitin ligase complex
Cellular ComponentHrd1p ubiquitin ligase ERAD-L complex
Cellular Componentlipid droplet
Cellular Componentmitochondrial outer membrane
Cellular Componentplasma membrane
Molecular Functionprotein-macromolecule adaptor activity
Molecular Functionubiquitin binding
Biological ProcessERAD pathway
Biological Processlipid droplet organization
Biological Processmitochondria-associated ubiquitin-dependent protein catabolic process
Biological Processmitochondrial protein processing
Biological Processproteasome-mediated ubiquitin-dependent protein catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

    • 3.A.16.1.6the endoplasmic reticular retrotranslocon (er-rt) family

Names & Taxonomy

Protein names

  • Recommended name
    UBX domain-containing protein 2
  • Alternative names
    • Secretion lowering protein 1

Gene names

    • Name
      UBX2
    • Synonyms
      SEL1
    • ORF names
      YM9571.05
    • Ordered locus names
      YML013W

Organism names

Accessions

  • Primary accession
    Q04228
  • Secondary accessions
    • D6VZG1

Proteomes

Organism-specific databases

Subcellular Location

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-80Cytoplasmic
Transmembrane81-101Helical
Topological domain102-151Lumenal
Transmembrane152-172Helical
Topological domain173-584Cytoplasmic

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002109991-584UBX domain-containing protein 2

Proteomic databases

PTM databases

Interaction

Subunit

Component of the DOA10 ubiquitin ligase complex which contains E3 ligase SSM4/DOA10 and CDC48-binding protein UBX2/SEL1 (PubMed:16873066).
Component of the HRD1 ubiquitin ligase complex which contains the E3 ligase HRD1, its cofactors HRD3, USA1 and DER1, substrate recruiting factor YOS9 and UBX2 (PubMed:16873066).
In ERAD-L, HRD3 and YOS9 jointly bind misfolded glycoproteins in the endoplasmic reticulum (ER) lumen (PubMed:32327568).
Movement of ERAD-L substrates through the ER membrane is facilitated by HRD1 and DER1 which have lateral gates facing each other and which distort the membrane region between the lateral gates, making it much thinner than a normal phospholipid bilayer (PubMed:32327568).
Substrates insert into the membrane as a hairpin loop with one strand interacting with DER1 and the other with HRD1 (PubMed:32327568).
Both the DOA10 and HRD1 ubiquitin ligase complexes interact with the heterotrimeric CDC48-NPL4-UFD1 ATPase complex which is recruited by UBX2 via its interaction with CDC48 and which moves ubiquitinated substrates to the cytosol for targeting to the proteasome (PubMed:15258615, PubMed:16179952, PubMed:16179953, PubMed:16873066).

Binary interactions

View interactors in UniProtKB
View CPX-3070 in Complex Portal

Protein-protein interaction databases

Miscellaneous

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain426-570UBX

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    584
  • Mass (Da)
    66,761
  • Last updated
    1997-11-01 v1
  • Checksum
    DA4DDD3D624C17B8
MPVVNHEDSEFHLSHTEEDKLNEFQVITNFPPEDLPDVVRLLRNHGWQLEPALSRYFDGEWKGEPDQMGEPTQTSTPMAETLVPPALGPRPLLFTASLPVVRPLPANFRNDFRTIGLNGRSNTVWSMFESFSYDGNPFLFILLLIPRIINRLSATIFTFFCTLLSLHSISGGGNSGKPKISKVPKAPTRETHIPLAEILGDTKDKDAFCELKSFKPDISFNEALRIAKEEFKFMLLILVGDTYDTDTDTVDVNSKLLLEKILLNKKTLQYLRKIDNDLIIYLKCVHELEPWLVARQLGVRNTPEIFLIANVANKASHSETLPSQRLSILGKLKVNSLNRFLQSLTNVVEKYTPELVVNKTEMHELRMSREIKKLQEDAYKKSLEMDRIKAIEKEKSLKHAQDLKLNSTARQLKWLKACIDEIQPFETTGKQATLQFRTSSGKRFVKKFPSMTTLYQIYQSIGCHIYLAVYSSDPAEWSNALQDKIRQLSADDDMLCFKEGQLETATATTIEELGHIINNELTSFDLERGKLEFDFELVSPFPKYTVHPNEHMSVDQVPQLWPNGSLLVEALDEEDEEDEENEEQ

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z49810
EMBL· GenBank· DDBJ
CAA89939.1
EMBL· GenBank· DDBJ
Genomic DNA
BK006946
EMBL· GenBank· DDBJ
DAA09885.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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