Q03AY7 · Q03AY7_LACP3
- ProteinMultifunctional fusion protein
- GenemurI
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids484 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Provides the (R)-glutamate required for cell wall biosynthesis.
Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.
Catalytic activity
- ITP + H2O = IMP + diphosphate + H+
Cofactor
Note: Binds 1 Mg2+ ion per subunit.
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 10-11 | substrate | ||||
Sequence: DS | ||||||
Binding site | 42-43 | substrate | ||||
Sequence: YG | ||||||
Active site | 73 | Proton donor/acceptor | ||||
Sequence: C | ||||||
Binding site | 74-75 | substrate | ||||
Sequence: NT | ||||||
Active site | 184 | Proton donor/acceptor | ||||
Sequence: C | ||||||
Binding site | 185-186 | substrate | ||||
Sequence: TH | ||||||
Binding site | 289-294 | substrate | ||||
Sequence: SKNQGK | ||||||
Binding site | 322 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Active site | 351 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 351 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 352 | substrate | ||||
Sequence: S | ||||||
Binding site | 436-439 | substrate | ||||
Sequence: FGYD | ||||||
Binding site | 459 | substrate | ||||
Sequence: K | ||||||
Binding site | 464-465 | substrate | ||||
Sequence: HR |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | dITP diphosphatase activity | |
Molecular Function | glutamate racemase activity | |
Molecular Function | ITP diphosphatase activity | |
Molecular Function | metal ion binding | |
Molecular Function | nucleotide binding | |
Molecular Function | ribonucleoside triphosphate phosphatase activity | |
Molecular Function | XTP diphosphatase activity | |
Biological Process | cell wall organization | |
Biological Process | nucleotide metabolic process | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | purine nucleoside triphosphate catabolic process | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameMultifunctional fusion protein
Including 2 domains:
- Recommended namedITP/XTP pyrophosphatase
- EC number
- Alternative names
- Recommended nameGlutamate racemase
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Lactobacillales > Lactobacillaceae > Lacticaseibacillus
Accessions
- Primary accessionQ03AY7
Proteomes
PTM/Processing
Proteomic databases
Interaction
Structure
Sequence
- Sequence statusComplete
- Length484
- Mass (Da)51,986
- Last updated2006-11-14 v1
- Checksum88907E30D146082A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000423 EMBL· GenBank· DDBJ | ABJ69635.1 EMBL· GenBank· DDBJ | Genomic DNA |