Q03689 · HETS_PODAS

Function

function

Responsible for heterokaryon incompatibility, a process that ensures that during spontaneous, vegetative cell fusion only compatible cells from the same colony survive (non-self-recognition). Forms a prion for the non-Mendelian trait [het-s]. Interacts with het-S from incompatible cells to trigger a lethal reaction that prevents the formation of viable heterokaryons. It is unknown if the native, soluble protein has a cellular function.

Miscellaneous

[Het-s] is the prion form of het-s (PubMed:9275200).
[Het-s] is the result of a conformational change of the cellular het-s protein that becomes self-propagating and infectious. This conformational change generates a form of het-S that assembles into amyloid fibrils (PubMed:12032295).
[Het-s] is transmitted as a non-Mendelian cytoplasmic element. On vegetative cell fusion with neutral [Het-s*] strains, the prion spreads throughout the cellular network and converts the non-prion form of het-s to a prion state, making the strains acquire the [het-s] phenotype (PubMed:9275200).
Interacts with het-S to trigger incompatibility (PubMed:12032295).
The protein present in [Het-s] strains is more resistant to proteinase K than that present in [Het-s*] mycelium (PubMed:9275200).
In P.anserina, the het-s locus exists as 2 incompatible alleles, het-s and het-S. Strains of het-s genotype (e.g. A, C, s, and V) can display 2 distinct phenoypes, the neutral (prion-free) [Het-s*], which is compatible with het-S strains (e.g. D, S, U, and X), and the reactive [Het-s] phenotype, which causes rapid cell death in het-S strains. Although the two alleles het-s and het-S differ from each other by 14 amino acids, vegetative incompatibility between s and S strains can be attributed to a single amino acid difference in the proteins encoded by the het-s locus (PubMed:8224826).
A sequence for the het-S allele can be found in strain S (AC B2ACC7).

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionidentical protein binding

Keywords

Protein family/group databases

    • 1.C.104.1.1the heterokaryon incompatibility prion/amyloid protein (het-s) family

Names & Taxonomy

Protein names

  • Recommended name
    Heterokaryon incompatibility protein s
  • Alternative names
    • Small s protein
    • Vegetative incompatibility protein s

Gene names

    • Name
      het-s
    • Synonyms
      small s

Organism names

  • Taxonomic identifier
  • Strains
    • s / FGSC 6710
    • A
    • C
    • V
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Sordariomycetidae > Sordariales > Podosporaceae > Podospora

Accessions

  • Primary accession
    Q03689
  • Secondary accessions
    • Q01531

Organism-specific databases

Subcellular Location

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis23Converts its specificity to [Het-S]; when associated with H-33.
Mutagenesis33Converts its specificity to [Het-S]; when associated with A-23.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004175691-289Heterokaryon incompatibility protein s

Interaction

Subunit

Homodimer. Forms heterodimers with het-S.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q03689het-s Q036893EBI-15800884, EBI-15800884

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-227Globular domain
Region218-289Prion domain (PrD)

Domain

The globular domain is dispensable for prion formation and incompatibility function. However, the het-S globular domain, but not the het-s globular domain, is essential for programmed cell death.
The prion domain (PrD) is unstructured in its native, soluble form, and forms a form a beta solenoid with a hydrophobic core in its amyloid form. It is both necessary and sufficient for amyloid formation and prion propagation.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    289
  • Mass (Da)
    31,978
  • Last updated
    2004-07-05 v3
  • Checksum
    D37468804C3DC793
MSEPFGIVAGALNVAGLFNNCVDCFEYVQLGRPFGRDYERCQLRLDIAKARLSRWGEAVKINDDPRFHSDAPTDKSVQLAKSIVEEILLLFESAQKTSKRYELVADQQDLVVFEDKDMKPIGRALHRRLNDLVSRRQKQTSLAKKTAWALYDGKSLEKIVDQVARFVDELEKAFPIEAVCHKLAEIEIEEVEDEASLTILKDAAGGIDAAMSDAAAQKIDAIVGRNSAKDIRTEERARVQLGNVVTAAALHGGIRISDQTTNSVETVVGKGESRVLIGNEYGGKGFWDN

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
M38529
EMBL· GenBank· DDBJ
AAB94631.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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