Q03213 · HOT1_YEAST
- ProteinHigh-osmolarity-induced transcription protein 1
- GeneHOT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids719 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Required for a complete transcriptional response to osmotic stress, through recruitment of HOG1 followed by pol II recruitment to the promoters of GPD1 and other HOG-dependent genes.
Miscellaneous
Present with 149 molecules/cell in log phase SD medium.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Molecular Function | DNA-binding transcription factor activity, RNA polymerase II-specific | |
Molecular Function | RNA polymerase II cis-regulatory region sequence-specific DNA binding | |
Molecular Function | RNA polymerase II general transcription initiation factor activity | |
Biological Process | cellular response to osmotic stress | |
Biological Process | positive regulation of ribosomal protein gene transcription by RNA polymerase II | |
Biological Process | positive regulation of transcription by RNA polymerase II | |
Biological Process | RNA polymerase II preinitiation complex assembly |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameHigh-osmolarity-induced transcription protein 1
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ03213
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 30 | Impairs HOT1 phosphorylation; when associated with A-70; A-153; A-360 and A-410. | ||||
Sequence: S → A | ||||||
Mutagenesis | 70 | Impairs HOT1 phosphorylation; when associated with A-30; A-153; A-360 and A-410. | ||||
Sequence: S → A | ||||||
Mutagenesis | 153 | Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-360 and A-410. | ||||
Sequence: S → A | ||||||
Mutagenesis | 360 | Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-410. | ||||
Sequence: S → A | ||||||
Mutagenesis | 410 | Impairs HOT1 phosphorylation; when associated with A-30; A-70; A-153 and A-360. | ||||
Sequence: S → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 27 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000203317 | 1-719 | High-osmolarity-induced transcription protein 1 | |||
Sequence: MSGMGIAILCIVRTKIYRITISFDYSTLMSPFFLFLMMPTTLKDGYRMNSQVNEDAIGINLDLSLPTHISPTTGSESASGSNASTLRNDGNALDGGLLRTSAAISAPTGTSQPTETIGEKLSNEERVNSNVSASNSTTAGTGRMLSQSLTNDSPSNEISTDQLKIFQRMDEMSARMIEMEESFNKLSNKIAEQNTMVLNLKQDNYKVMNKLNILLKLVAQPSARPSTNNAQNKLAIELLNSISAVSSAYLQKMQNNGSGRQHTADLCTGDSNTHSGINQHRTTNGTIDVNTNTAQLNNQFSNALNTILPDQQHNRNNVSQNINQSLPNRQLGPVINTQANQNQSQVLIHNTNTHQQVNRSPISFPNASTDKPFKLNPNGIKRRRRNTQSNNNASTNDHASAAQKPISALSPLTNSHNSTTSMNYTNSSIHSGVTSASNSFHDLNSLNNFGTTTALSLPSLALDNASFPPNQNVIPPIINNTQQPLSFSQLINQDSTTSELLPSGKSGVNTNIVNRNRASTLPSYPKPMTVKSNVDDDGYQEDDDDDGDDEGDGRDNEEDSTAEEDEVDDEIETDMKNASINKRRRSLHHKKSNSLNGRRKLHGESATKPNINSDLHYRILKAPTDVKTIWEEYDTGIRGKPSIKHLEAKYGNKWRLNKNKKTFSRRKRLYKFILNGMERGKTAQEMIETLENKRLYKDDEDGEVKKRTIGWLQESLAGI | ||||||
Modified residue | 146 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 153 | Phosphoserine | ||||
Sequence: S |
Post-translational modification
Hyperphosphorylated during acute stress.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Subunit
Interacts with HOG1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q03213 | HOG1 P32485 | 4 | EBI-27376, EBI-8437 | |
BINARY | Q03213 | RPD3 P32561 | 3 | EBI-27376, EBI-15864 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 68-89 | Polar residues | ||||
Sequence: HISPTTGSESASGSNASTLRND | ||||||
Region | 68-90 | Disordered | ||||
Sequence: HISPTTGSESASGSNASTLRNDG | ||||||
Region | 102-159 | Disordered | ||||
Sequence: AAISAPTGTSQPTETIGEKLSNEERVNSNVSASNSTTAGTGRMLSQSLTNDSPSNEIS | ||||||
Compositional bias | 103-159 | Polar residues | ||||
Sequence: AISAPTGTSQPTETIGEKLSNEERVNSNVSASNSTTAGTGRMLSQSLTNDSPSNEIS | ||||||
Compositional bias | 353-373 | Polar residues | ||||
Sequence: THQQVNRSPISFPNASTDKPF | ||||||
Region | 353-402 | Disordered | ||||
Sequence: THQQVNRSPISFPNASTDKPFKLNPNGIKRRRRNTQSNNNASTNDHASAA | ||||||
Compositional bias | 382-402 | Polar residues | ||||
Sequence: RRRRNTQSNNNASTNDHASAA | ||||||
Compositional bias | 496-527 | Polar residues | ||||
Sequence: TTSELLPSGKSGVNTNIVNRNRASTLPSYPKP | ||||||
Region | 496-610 | Disordered | ||||
Sequence: TTSELLPSGKSGVNTNIVNRNRASTLPSYPKPMTVKSNVDDDGYQEDDDDDGDDEGDGRDNEEDSTAEEDEVDDEIETDMKNASINKRRRSLHHKKSNSLNGRRKLHGESATKPN | ||||||
Compositional bias | 537-572 | Acidic residues | ||||
Sequence: DGYQEDDDDDGDDEGDGRDNEEDSTAEEDEVDDEIE | ||||||
Compositional bias | 582-596 | Basic residues | ||||
Sequence: KRRRSLHHKKSNSLN |
Sequence similarities
Belongs to the HOT1 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length719
- Mass (Da)79,416
- Last updated1997-11-01 v1
- Checksum4652BE93743D5A54
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 68-89 | Polar residues | ||||
Sequence: HISPTTGSESASGSNASTLRND | ||||||
Compositional bias | 103-159 | Polar residues | ||||
Sequence: AISAPTGTSQPTETIGEKLSNEERVNSNVSASNSTTAGTGRMLSQSLTNDSPSNEIS | ||||||
Compositional bias | 353-373 | Polar residues | ||||
Sequence: THQQVNRSPISFPNASTDKPF | ||||||
Compositional bias | 382-402 | Polar residues | ||||
Sequence: RRRRNTQSNNNASTNDHASAA | ||||||
Compositional bias | 496-527 | Polar residues | ||||
Sequence: TTSELLPSGKSGVNTNIVNRNRASTLPSYPKP | ||||||
Compositional bias | 537-572 | Acidic residues | ||||
Sequence: DGYQEDDDDDGDDEGDGRDNEEDSTAEEDEVDDEIE | ||||||
Compositional bias | 582-596 | Basic residues | ||||
Sequence: KRRRSLHHKKSNSLN |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z49808 EMBL· GenBank· DDBJ | CAA89905.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006946 EMBL· GenBank· DDBJ | DAA10068.1 EMBL· GenBank· DDBJ | Genomic DNA |