Q03096 · EST3_YEAST
- ProteinTelomere replication protein EST3
- GeneEST3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids181 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Component of the telomerase complex involved in telomere replication. Stimulates RNA/DNA heteroduplex unwinding which favors the telomere replication by the telomerase.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromosome, telomeric region | |
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Cellular Component | telomerase holoenzyme complex | |
Molecular Function | DNA/RNA helicase activity | |
Molecular Function | GTPase activity | |
Molecular Function | telomeric DNA binding | |
Biological Process | telomere maintenance via telomerase | |
Biological Process | viral translational frameshifting |
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTelomere replication protein EST3
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ03096
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 3 | Causes telomere shortening. | ||||
Sequence: K → A | ||||||
Mutagenesis | 21 | Causes telomere shortening. | ||||
Sequence: W → A | ||||||
Mutagenesis | 49 | Reduces ability to dimerize and causes telomere shortening. | ||||
Sequence: D → A | ||||||
Mutagenesis | 68 | Reduces ability to dimerize and causes telomere shortening. | ||||
Sequence: K → A | ||||||
Mutagenesis | 68 | Causes telomere shortening. | ||||
Sequence: K → E, S, or Y | ||||||
Mutagenesis | 71 | Causes telomere shortening, but does not impair interaction with the telomerase RNP. | ||||
Sequence: K → A or E | ||||||
Mutagenesis | 72 | Causes telomere shortening. | ||||
Sequence: F → A | ||||||
Mutagenesis | 82 | Causes telomere shortening. | ||||
Sequence: A → E | ||||||
Mutagenesis | 86 | Causes progressive telomere shortening and cell senescence. | ||||
Sequence: D → A | ||||||
Mutagenesis | 87 | Causes telomere shortening. | ||||
Sequence: S → A | ||||||
Mutagenesis | 95 | Causes telomere shortening. | ||||
Sequence: F → A | ||||||
Mutagenesis | 103 | Causes telomere shortening. | ||||
Sequence: F → A | ||||||
Mutagenesis | 104 | Reduces binding to DNA and RNA, and causes progressive telomere shortening and cell senescence. | ||||
Sequence: E → A | ||||||
Mutagenesis | 104 | Impairs interaction with the telomerase RNP and causes progressive telomere shortening and cell senescence. | ||||
Sequence: E → R | ||||||
Mutagenesis | 110 | Causes progressive telomere shortening and cell senescence, but does not impair interaction with the telomerase RNP. | ||||
Sequence: R → A or E | ||||||
Mutagenesis | 114 | Impairs interaction with the telomerase RNP and causes telomere shortening. | ||||
Sequence: E → A or K | ||||||
Mutagenesis | 115 | Impairs interaction with the telomerase RNP and causes telomere shortening. | ||||
Sequence: T → A | ||||||
Mutagenesis | 117 | Impairs interaction with the telomerase RNP and causes telomere shortening. | ||||
Sequence: N → A or K | ||||||
Mutagenesis | 164 | Causes telomere shortening, but does not impair interaction with the telomerase RNP. | ||||
Sequence: D → A or R | ||||||
Mutagenesis | 166 | Reduces ability to dimerize and causes telomere shortening. | ||||
Sequence: D → A | ||||||
Mutagenesis | 166 | Impairs interaction with the telomerase RNP and causes telomere shortening. | ||||
Sequence: D → R | ||||||
Mutagenesis | 168 | Causes telomere shortening. | ||||
Sequence: V → A, D, or Y |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 8 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000021206 | 1-181 | Telomere replication protein EST3 | |||
Sequence: MPKVILESHSKPTDSVFLQPWIKALIEDNSEHDQYHPSGHVIPSLTKQDLALPHMSPTILTNPCHFAKITKFYNVCDYKVYASIRDSSHQILVEFSQECVSNFERTHNCRITSETTNCLMIIGDADLVYVTNSRAMSHFKICLSNISSKEIVPVLNVNQATIFDIDQVGSLSTFPFVYKYL |
Proteomic databases
Interaction
Subunit
Homodimer. Component of the telomerase complex composed of EST1, EST2, EST3 and the RNA component TLC1. Interacts with the telomerase RNA component TLC1 in presence of EST2.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q03096 | EST2 Q06163 | 3 | EBI-6691, EBI-3764464 |
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Ribosomal frameshifting.
Q03096-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- NoteProduced by ribosomal frameshifting between codon Leu-92 and Val-93.
- Length181
- Mass (Da)20,555
- Last updated2002-11-01 v2
- Checksum18AF3DCEEE65DB84
Q03096-2
- Name2
- NoteProduced from conventional translation of the YIL009C-A ORF. There is no apparent role for isoform 2 in the cell.
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z38113 EMBL· GenBank· DDBJ | CAA86240.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. | |
BK006942 EMBL· GenBank· DDBJ | DAA08536.1 EMBL· GenBank· DDBJ | Genomic DNA |