Q02899 · OYE1_SACPS
- ProteinNADPH dehydrogenase 1
- GeneOYE1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids400 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Flavin-dependent enoate reductase that catalyzes the chemo- and stereoslective hydrogenation of electron-poor alkenes. The enzyme is reduced by NADPH, and oxygen, quinones, and alpha,beta-unsaturated aldehydes and ketones can act as electron acceptors to complete catalytic turnover. The physiological oxidant remains elusive.
Catalytic activity
- A + H+ + NADPH = AH2 + NADP+
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
11.4 μM | alpha-NADPH | |||||
7.7 μM | beta-NADPH | |||||
1.18 mM | oxygen (with alpha-NADPH a substrate) | |||||
0.88 mM | oxygen (with beta-NADPH a substrate) |
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 38 | FMN (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 115 | FMN (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 192 | substrate | ||||
Sequence: H | ||||||
Binding site | 195 | substrate | ||||
Sequence: N | ||||||
Active site | 197 | Proton donor | ||||
Sequence: Y | ||||||
Binding site | 244 | FMN (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 349 | FMN (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 376 | substrate | ||||
Sequence: Y |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | FMN binding | |
Molecular Function | NADPH dehydrogenase activity | |
Molecular Function | pentaerythritol trinitrate reductase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADPH dehydrogenase 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ02899
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 115 | Strong reduction of substrate binding. | ||||
Sequence: Q → N | ||||||
Mutagenesis | 192 | Strong reduction of substrate binding. Reduced oxidation of NADPH. | ||||
Sequence: H → N | ||||||
Mutagenesis | 197 | Reduces oxidative half-reaction with 2-cyclohexenone 6-fold. | ||||
Sequence: Y → F |
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Chain | PRO_0000194473 | 2-400 | NADPH dehydrogenase 1 | |||
Sequence: SFVKDFKPQALGDTNLFKPIKIGNNELLHRAVIPPLTRMRALHPGNIPNRDWAVEYYTQRAQRPGTMIITEGAFISPQAGGYDNAPGVWSEEQMVEWTKIFNAIHEKKSFVWVQLWVLGWAAFPDNLARDGLRYDSASDNVFMDAEQEAKAKKANNPQHSLTKDEIKQYIKEYVQAAKNSIAAGADGVEIHSANGYLLNQFLDPHSNTRTDEYGGSIENRARFTLEVVDALVEAIGHEKVGLRLSPYGVFNSMSGGAETGIVAQYAYVAGELEKRAKAGKRLAFVHLVEPRVTNPFLTEGEGEYEGGSNDFVYSIWKGPVIRAGNFALHPEVVREEVKDKRTLIGYGRFFISNPDLVDRLEKGLPLNKYDRDTFYQMSAHGYIDYPTYEEALKLGWDKK |
Interaction
Subunit
Homodimer or heterodimer.
Structure
Sequence
- Sequence statusComplete
- Length400
- Mass (Da)45,015
- Last updated2007-01-23 v3
- Checksum0F3CA987E3EE1310
Mass Spectrometry
Keywords
- Technical term