Q02883 · FMP30_YEAST
- ProteinN-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, mitochondrial
- GeneFMP30
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids468 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs).
Miscellaneous
Present with 178 molecules/cell in log phase SD medium.
Catalytic activity
- H2O + N-acyl-1,2-diacyl-sn-glycero-3-phosphoethanolamine = a 1,2-diacyl-sn-glycero-3-phosphate + an N-acylethanolamine + H+
Cofactor
Note: Binds 1 or 2 zinc ions per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 265 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 267 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 269 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 270 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 332 | Zn2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 425 | Zn2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | membrane-bounded organelle | |
Cellular Component | mitochondrial inner membrane | |
Cellular Component | mitochondrion | |
Molecular Function | N-acylphosphatidylethanolamine-specific phospholipase D activity | |
Molecular Function | zinc ion binding | |
Biological Process | N-acylethanolamine metabolic process | |
Biological Process | N-acylphosphatidylethanolamine metabolic process | |
Biological Process | phospholipid catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameN-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, mitochondrial
- EC number
- Short namesNAPE-PLD; NAPE-hydrolyzing phospholipase D
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Saccharomycotina > Saccharomycetes > Saccharomycetales > Saccharomycetaceae > Saccharomyces
Accessions
- Primary accessionQ02883
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 54-76 | Helical | ||||
Sequence: YARILLLSVLVPYTGYAFYVSLA |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-39 | Mitochondrion | ||||
Sequence: MNFVTCHVQMRLLLQRRLVRLRESELFRPQTSLSTFKRH | ||||||
Chain | PRO_0000238633 | 40-468 | N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, mitochondrial | |||
Sequence: ASQKTRPIQKCSRKYARILLLSVLVPYTGYAFYVSLATVKQIDLRNEMCQRLEENNNEVTYKGSLLKYSPLEVLGRFENPFEEYRIQTVFEFFANRVFELFERNRGGIPRDVHQMNKLMPVHKPTWGPNLVDVDPAEETALPLECKVLDELHIPTAVEENEGSKCPVYNTWLGQSCNYTVYNGLRILTDPLFSDFLIHKTLGPKRITQMPSQITEVPKPDIILVSHNHPDHLDLESLEYWSGKDSPLWIVPKGMKSYMTSNGCDNVLELSWWETLQVKKNNEIYHISATPAMHWSGRSLLDTNKSLWCSFLLTHHGNPILFHAGDTGYVKDLFVRIKERFGKGCKLALLPCGQYCPEWHQKPRHINPQEVLKIMKDLEARNVLGVHWGTFVLSGEYFLEPKEKLEMLAEWGGFKDRCYCPELGKTECFD |
Proteomic databases
Expression
Induction
Expressed periodically during the glycolytic and respiratory oscillations cycles.
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length468
- Mass (Da)54,368
- Last updated1996-11-01 v1
- ChecksumC5E6D8817C16791A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U43281 EMBL· GenBank· DDBJ | AAB68197.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BK006949 EMBL· GenBank· DDBJ | DAA11330.1 EMBL· GenBank· DDBJ | Genomic DNA |