Q02819 · NUCB1_MOUSE
- ProteinNucleobindin-1
- GeneNucb1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids459 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Major calcium-binding protein of the Golgi which may have a role in calcium homeostasis (By similarity).
Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins) (By similarity).
Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates alpha subunits of guanine nucleotide-binding proteins (G proteins) (By similarity).
Miscellaneous
Discovered as DNA-binding protein in the serum of lupus-prone mice.
Features
Showing features for dna binding, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 171-217 | |||||
Sequence: HHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPK | ||||||
Binding site | 252 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 254 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 256 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 263 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 304 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 306 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 308 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 315 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | early endosome | |
Cellular Component | endoplasmic reticulum-Golgi intermediate compartment | |
Cellular Component | extracellular region | |
Cellular Component | extracellular space | |
Cellular Component | extrinsic component of Golgi membrane | |
Cellular Component | Golgi cisterna membrane | |
Cellular Component | Golgi-associated vesicle | |
Cellular Component | lumenal side of Golgi membrane | |
Cellular Component | trans-Golgi network | |
Molecular Function | calcium ion binding | |
Molecular Function | DNA binding | |
Molecular Function | G-protein alpha-subunit binding | |
Molecular Function | guanyl-nucleotide exchange factor activity | |
Biological Process | regulation of protein targeting | |
Biological Process | small GTPase-mediated signal transduction |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNucleobindin-1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ02819
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus, cis-Golgi network membrane ; Peripheral membrane protein
Note: A small fraction of the protein may be cytoplasmic. In bone at least part of it is found in the extracellular matrix and in the culture medium of calvaria explants (By similarity).
In lupus prone, but not in normal mice, at least part of it is in the serum where it induces the formation of autoantibodies including anti-DNA antibodies
In lupus prone, but not in normal mice, at least part of it is in the serum where it induces the formation of autoantibodies including anti-DNA antibodies
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-25 | |||||
Sequence: MPTSVPRGAPFLLLPPLLMLSAVLA | ||||||
Chain | PRO_0000004163 | 26-459 | Nucleobindin-1 | |||
Sequence: VPVDRAAPPQEDSQATETPDTGLYYHRYLQEVINVLETDGHFREKLQAANAEDIKSGKLSQELDFVSHNVRTKLDELKRQEVSRLRMLLKAKMDAKQEPNLQVDHMNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWKTVEMSPAYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQLQEQSAPPSKPDGQLQFRADTDDAPVPAPAGDQKDVPASEKKVPEQPPELPQLDSQHL | ||||||
Modified residue | 85 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 147 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 368 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 456 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Lymphoid cells as well as other somatic cells, such as liver and kidney cells.
Gene expression databases
Interaction
Subunit
Interacts (via GBA motif) with guanine nucleotide-binding protein G(i) alpha subunits GNAI1, GNAI2 and GNAI3 with higher affinity for GNAI1 and GNAI3 than for GNAI2. Preferentially interacts with inactive rather than active GNAI3. Interaction with GNAI3 is inhibited when NUCB1 binds calcium, probably due to a conformational change which renders the GBA motif inaccessible.
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for coiled coil, compositional bias, region, domain, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Coiled coil | 149-217 | |||||
Sequence: EARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLQEQQRRHREHPK | ||||||
Compositional bias | 192-218 | Basic and acidic residues | ||||
Sequence: SLGEEQRKEAERKLQEQQRRHREHPKV | ||||||
Region | 192-220 | Disordered | ||||
Sequence: SLGEEQRKEAERKLQEQQRRHREHPKVNV | ||||||
Region | 227-320 | Binds to GNAI2 and GNAI3 | ||||
Sequence: LKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLAST | ||||||
Domain | 239-274 | EF-hand 1 | ||||
Sequence: PNRFNPKTFFILHDINSDGVLDEQELEALFTKELEK | ||||||
Domain | 291-326 | EF-hand 2 | ||||
Sequence: ERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFG | ||||||
Motif | 302-332 | GBA | ||||
Sequence: NVDTNQDRLVTLEEFLASTQRKEFGDTGEGW | ||||||
Coiled coil | 340-407 | |||||
Sequence: AYTEEELKRFEEELAAREAELNARAQRLSQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQLQEQSA | ||||||
Region | 368-459 | Disordered | ||||
Sequence: SQETEALGRSQDRLEAQKRELQQAVLQMEQRKQQLQEQSAPPSKPDGQLQFRADTDDAPVPAPAGDQKDVPASEKKVPEQPPELPQLDSQHL | ||||||
Compositional bias | 390-412 | Polar residues | ||||
Sequence: QAVLQMEQRKQQLQEQSAPPSKP |
Domain
The EF-hand domains are unfolded in the absence of Ca2+ and fold upon Ca2+ addition.
The GBA (G-alpha binding and activating) motif mediates binding to the alpha subunits of guanine nucleotide-binding proteins (G proteins).
Sequence similarities
Belongs to the nucleobindin family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length459
- Mass (Da)53,409
- Last updated2001-11-02 v2
- Checksum1CC1102D216CC60B
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1C7CYU3 | A0A1C7CYU3_MOUSE | Nucb1 | 455 | ||
A0A1B0GR92 | A0A1B0GR92_MOUSE | Nucb1 | 220 | ||
A0A1B0GR41 | A0A1B0GR41_MOUSE | Nucb1 | 271 | ||
A0A1B0GT83 | A0A1B0GT83_MOUSE | Nucb1 | 121 | ||
A0A1B0GS16 | A0A1B0GS16_MOUSE | Nucb1 | 170 |
Features
Showing features for sequence conflict, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 42 | in Ref. 2; BAB25383 | ||||
Sequence: E → D | ||||||
Sequence conflict | 146 | in Ref. 1; AA sequence | ||||
Sequence: H → E | ||||||
Sequence conflict | 151 | in Ref. 1; AA sequence | ||||
Sequence: R → I | ||||||
Compositional bias | 192-218 | Basic and acidic residues | ||||
Sequence: SLGEEQRKEAERKLQEQQRRHREHPKV | ||||||
Sequence conflict | 390-393 | in Ref. 1; AAA39842 | ||||
Sequence: Missing | ||||||
Compositional bias | 390-412 | Polar residues | ||||
Sequence: QAVLQMEQRKQQLQEQSAPPSKP |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M96823 EMBL· GenBank· DDBJ | AAA39842.1 EMBL· GenBank· DDBJ | mRNA | ||
AK002630 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
AK004886 EMBL· GenBank· DDBJ | BAB23644.1 EMBL· GenBank· DDBJ | mRNA | ||
AK007976 EMBL· GenBank· DDBJ | BAB25383.1 EMBL· GenBank· DDBJ | mRNA | ||
AK140710 EMBL· GenBank· DDBJ | BAE24451.1 EMBL· GenBank· DDBJ | mRNA | ||
AK160981 EMBL· GenBank· DDBJ | BAE36129.1 EMBL· GenBank· DDBJ | mRNA | ||
AK164088 EMBL· GenBank· DDBJ | BAE37619.1 EMBL· GenBank· DDBJ | mRNA | ||
BC072554 EMBL· GenBank· DDBJ | AAH72554.1 EMBL· GenBank· DDBJ | mRNA |