Q02084 · A33_PLEWA
- ProteinZinc-binding protein A33
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids625 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
May be a nuclear regulatory protein that is stored in the germinal vesicle for use during early embryogenesis and may play a role in the synthesis or processing of pre-mRNA during oogenesis. Binds 3 Zn2+ ions.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chromosome | |
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | RNA binding | |
Molecular Function | ubiquitin protein ligase activity | |
Molecular Function | zinc ion binding | |
Biological Process | protein ubiquitination | |
Biological Process | regulation of DNA-templated transcription |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Recommended nameZinc-binding protein A33
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Caudata > Salamandroidea > Salamandridae > Pleurodelinae > Pleurodeles
Accessions
- Primary accessionQ02084
Subcellular Location
UniProt Annotation
GO Annotation
Note: Lampbrush chromosome loops and in some nucleoplasmic particles of the germinal vesicle.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 178 | No effect on intranuclear localization. | ||||
Sequence: C → F | ||||||
Mutagenesis | 202 | No effect on intranuclear localization. | ||||
Sequence: C → F | ||||||
Mutagenesis | 267 | No Zn2+ binding. Inhibits the targeting to lampbrush chromosome loops and to nucleoplasmic particles. | ||||
Sequence: H → N |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000055746 | 1-625 | Zinc-binding protein A33 | |||
Sequence: MANSTVAEPEKMEQSGTCEEDEEEENMEGDEDECDDDEEENMEPAETVTIGSTYKCRRSDNTLHAAEIIKTRKTKENAEEFYVHYVGLNRRQNEWVDKSRVLQAKQIKTEELNNTEDETNGVSDQSEGKAARSNKRKIEDGDGDQKKRKVDDEEDDFTEDLTCPLCRSLFKEPVILECGHNFCKHCIDKSWESASAFSCPECKEVLTERKYTTNRVLANLVKKAAVGVKDKDVKPKEKCDEHDERLKLFCKDDGTLACVICRDSLKHSNHNFLPIQDAVGVYRDQLIALVSPLETTMKENQKLKCDQSQKISLHRENIVDCKKHIECEFEKLHQFLREKEAKMVEDLNAEREGLLKDMEANLVKMTDNCEFIEEAISTTQSRLNESDPIAFLTDIKSFIEKCCEEHRKGVPAESVLVNKELSQGRFKGPLQYIIWKELKSVVQPGLAPLTLDPNTAHPNLVLSEGLTSVKYTDTKQQLPDNPKRFSQCILVLGAEGFDSGKHYWEVEVGNKTAWDVGMASESSNRKGKIKLNPKNGYWAIWLRNGNAFKALESPSKTLNLTSKPSKIGVYLDYEGGQVSFYNADDMSPIYTFNGSFTEKLYPYLSPFLQDSGKNAEPLKLVHTKL |
Expression
Developmental stage
It first appears on the chromosome loops and in the nucleoplasm of the germinal vesicle (GV). It is transmitted to the egg at GV breakdown and appears in embryonic nuclei at the mid-blastula stage and is found in many but not all nuclei at still later stages of embryogenesis.
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain, zinc finger, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-53 | Disordered | ||||
Sequence: MANSTVAEPEKMEQSGTCEEDEEEENMEGDEDECDDDEEENMEPAETVTIGST | ||||||
Compositional bias | 15-44 | Acidic residues | ||||
Sequence: SGTCEEDEEEENMEGDEDECDDDEEENMEP | ||||||
Domain | 48-101 | Tudor-knot | ||||
Sequence: VTIGSTYKCRRSDNTLHAAEIIKTRKTKENAEEFYVHYVGLNRRQNEWVDKSRV | ||||||
Region | 112-151 | Disordered | ||||
Sequence: LNNTEDETNGVSDQSEGKAARSNKRKIEDGDGDQKKRKVD | ||||||
Compositional bias | 129-151 | Basic and acidic residues | ||||
Sequence: KAARSNKRKIEDGDGDQKKRKVD | ||||||
Zinc finger | 163-203 | RING-type | ||||
Sequence: CPLCRSLFKEPVILECGHNFCKHCIDKSWESASAFSCPECK | ||||||
Zinc finger | 234-275 | B box-type | ||||
Sequence: KPKEKCDEHDERLKLFCKDDGTLACVICRDSLKHSNHNFLPI | ||||||
Coiled coil | 337-386 | |||||
Sequence: REKEAKMVEDLNAEREGLLKDMEANLVKMTDNCEFIEEAISTTQSRLNES | ||||||
Domain | 429-625 | B30.2/SPRY | ||||
Sequence: PLQYIIWKELKSVVQPGLAPLTLDPNTAHPNLVLSEGLTSVKYTDTKQQLPDNPKRFSQCILVLGAEGFDSGKHYWEVEVGNKTAWDVGMASESSNRKGKIKLNPKNGYWAIWLRNGNAFKALESPSKTLNLTSKPSKIGVYLDYEGGQVSFYNADDMSPIYTFNGSFTEKLYPYLSPFLQDSGKNAEPLKLVHTKL |
Domain
B box-type zinc binding domain is required for targeting this protein to lampbrush chromosome loops and for normal functioning of this protein in the oocyte.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length625
- Mass (Da)71,053
- Last updated2006-11-28 v2
- ChecksumBCE8003BB6BF5B13
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 15-44 | Acidic residues | ||||
Sequence: SGTCEEDEEEENMEGDEDECDDDEEENMEP | ||||||
Compositional bias | 129-151 | Basic and acidic residues | ||||
Sequence: KAARSNKRKIEDGDGDQKKRKVD |