Q017T9 · PURA_OSTTA

  • Protein
    Adenylosuccinate synthetase, chloroplastic
  • Gene
    PURA
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site69-75GTP (UniProtKB | ChEBI)
Active site70Proton acceptor
Binding site70Mg2+ (UniProtKB | ChEBI)
Binding site70-73IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site95-98IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site97Mg2+ (UniProtKB | ChEBI)
Binding site97-99GTP (UniProtKB | ChEBI)
Active site98Proton donor
Binding site187IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site201IMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site278IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site293IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site353-359substrate
Binding site357IMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site359GTP (UniProtKB | ChEBI)
Binding site385-387GTP (UniProtKB | ChEBI)
Binding site468-470GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Molecular Functionadenylosuccinate synthase activity
Molecular FunctionGTP binding
Molecular Functionmagnesium ion binding
Biological Process'de novo' AMP biosynthetic process
Biological ProcessIMP metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Adenylosuccinate synthetase, chloroplastic
  • EC number
  • Short names
    AMPSase
    ; AdSS
  • Alternative names
    • IMP--aspartate ligase

Gene names

    • Name
      PURA
    • Ordered locus names
      Ot06g00520

Organism names

  • Taxonomic identifier
  • Strain
    • OTTH0595
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Chlorophyta > Mamiellophyceae > Mamiellales > Bathycoccaceae > Ostreococcus

Accessions

  • Primary accession
    Q017T9
  • Secondary accessions
    • A0A090M6B2

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-54Chloroplast
ChainPRO_000039928055-480Adenylosuccinate synthetase, chloroplastic

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the adenylosuccinate synthetase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    480
  • Mass (Da)
    52,386
  • Last updated
    2015-10-14 v2
  • Checksum
    E5FDCF8B93A6E1B6
MATARVMVADRARAFGGTTATRARRDDQGRRVTIARGIPSRARVVVARASERAYAELGNVCAVLGSQWGDEGKGKLVDILAREYDVVARCQGGANAGHTIYDDNGKKYALHLVPSGILNENATCVVGNGVVVHLPGMFEEIDKLLQAGVDARGRMIVSDRAHLLFDLHKEIDGLREEELSGNKIGTTKRGIGPAYASKATRNGVRLGDIRDEERFATMLRALAADAAARFEGFQYDVEAEIVRYKEIAERIEPFIADTVEYINEAHDSGKKILVEGANATMLDLDFGTYPFVTSSNPALGGVCNGLGLAPRKFKTIIGVAKAYTTRVGAGPYPTELFGDVADKLRELGYEYGTTTGRPRRIGWLDMVALNYANLINGFTHLNITKLDVLSEMDELKIGVAYKLPNGKTTKSFPADIATLEKVETVYETLPGWKEDISKVRTWDDLPENAKKYVLRVEELAGVECKFIGVGPGRDAMVIKP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CAID01000006
EMBL· GenBank· DDBJ
CEF98222.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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