Q01728 · NAC1_RAT
- ProteinSodium/calcium exchanger 1
- GeneSlc8a1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids971 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Mediates the exchange of one Ca2+ ion against three to four Na+ ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca2+ levels and Ca2+-dependent cellular processes (PubMed:10894800, PubMed:12502557, PubMed:8422940, PubMed:8454039).
Contributes to Ca2+ transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca2+ levels due to release of Ca2+ stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca2+ from the cell during the next phase, so that cytoplasmic Ca2+ levels rapidly return to baseline. Required for normal embryonic heart development and the onset of heart contractions (By similarity).
Contributes to Ca2+ transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca2+ levels due to release of Ca2+ stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca2+ from the cell during the next phase, so that cytoplasmic Ca2+ levels rapidly return to baseline. Required for normal embryonic heart development and the onset of heart contractions (By similarity).
Catalytic activity
- Ca2+(in) + 3 Na+(out) = Ca2+(out) + 3 Na+(in)
Activity regulation
Activated by micromolar levels of Ca2+ (PubMed:12502557).
Isoform 4
Isoform 5
Isoform 6
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 417 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 417 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 417 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 453 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 453 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 478 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 479 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 479 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 479 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 479 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 481 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 483 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 483 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 483 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 486 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 530 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 531 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 532 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 532 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 548 | Ca2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 584 | Ca2+ 6 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 610 | Ca2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 610 | Ca2+ 6 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 611 | Ca2+ 6 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 612 | Ca2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 612 | Ca2+ 6 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 716 | Ca2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSodium/calcium exchanger 1
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ01728
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 33-71 | Extracellular | ||||
Sequence: DTEAETGGNETTECTGSYYCKKGVILPIWEPQDPSFGDK | ||||||
Transmembrane | 72-92 | Helical | ||||
Sequence: IARATVYFVAMVYMFLGVSII | ||||||
Topological domain | 93-133 | Cytoplasmic | ||||
Sequence: ADRFMSSIEVITSQEKEITIKKPNGETTKTTVRIWNETVSN | ||||||
Transmembrane | 134-154 | Helical | ||||
Sequence: LTLMALGSSAPEILLSVIEVC | ||||||
Topological domain | 155-167 | Extracellular | ||||
Sequence: GHNFTAGDLGPST | ||||||
Transmembrane | 168-188 | Helical | ||||
Sequence: IVGSAAFNMFIIIALCVYVVP | ||||||
Topological domain | 189-201 | Cytoplasmic | ||||
Sequence: DGETRKIKHLRVF | ||||||
Transmembrane | 202-222 | Helical | ||||
Sequence: FVTAAWSIFAYTWLYIILSVS | ||||||
Topological domain | 223-228 | Extracellular | ||||
Sequence: SPGVVE | ||||||
Transmembrane | 229-249 | Helical | ||||
Sequence: VWEGLLTFFFFPICVVFAWVA | ||||||
Topological domain | 250-798 | Cytoplasmic | ||||
Sequence: DRRLLFYKYVYKRYRAGKQRGMIIEHEGDRPASKTEIEMDGKVVNSHVDNFLDGALVLEVDERDQDDEEARREMARILKELKQKHPDKEIEQLIELANYQVLSQQQKSRAFYRIQATRLMTGAGNILKRHAADQARKAVSMHEVNMDVVENDPVSKVFFEQGTYQCLENCGTVALTIIRRGGDLTNTVFVDFRTEDGTANAGSDYEFTEGTVIFKPGETQKEIRVGIIDDDIFEEDENFLVHLSNVRVSSEVSEDGILDSNHVSAIACLGSPNTATITIFDDDHAGIFTFEEPVTHVSESIGIMEVKVLRTSGARGNVIIPYKTIEGTARGGGEDFEDTCGELEFQNDEIVKTISVKVIDDEEYEKNKTFFIEIGEPRLVEMSEKKALLLNELGGFTLTEGKKMYGQPVFRKVHARDHPIPSTVISISEEYDDKQPLTSKEEEERRIAEMGRPILGEHTKLEVIIEESYEFKSTVDKLIKKTNLALVVGTNSWREQFIEAITVSAGEDDDDDECGEEKLPSCFDYVMHFLTVFWKVLFAFVPPTEYWNG | ||||||
Transmembrane | 799-819 | Helical | ||||
Sequence: WACFIVSILMIGLLTAFIGDL | ||||||
Topological domain | 820-822 | Extracellular | ||||
Sequence: ASH | ||||||
Transmembrane | 823-843 | Helical | ||||
Sequence: FGCTIGLKDSVTAVVFVALGT | ||||||
Topological domain | 844-872 | Cytoplasmic | ||||
Sequence: SVPDTFASKVAATQDQYADASIGNVTGSN | ||||||
Transmembrane | 873-893 | Helical | ||||
Sequence: AVNVFLGIGVAWSIAAIYHAA | ||||||
Topological domain | 894-904 | Extracellular | ||||
Sequence: NGEQFKVSPGT | ||||||
Transmembrane | 905-925 | Helical | ||||
Sequence: LAFSVTLFTIFAFINVGVLLY | ||||||
Topological domain | 926-942 | Cytoplasmic | ||||
Sequence: RRRPEIGGELGGPRTAK | ||||||
Transmembrane | 943-963 | Helical | ||||
Sequence: LLTSSLFVLLWLLYIFFSSLE | ||||||
Topological domain | 964-971 | Extracellular | ||||
Sequence: AYCHIKGF |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain, glycosylation, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-32 | |||||
Sequence: MLRLSLPPNVSMGFRLVTLVALLFTHVDHITA | ||||||
Chain | PRO_0000019381 | 33-971 | Sodium/calcium exchanger 1 | |||
Sequence: DTEAETGGNETTECTGSYYCKKGVILPIWEPQDPSFGDKIARATVYFVAMVYMFLGVSIIADRFMSSIEVITSQEKEITIKKPNGETTKTTVRIWNETVSNLTLMALGSSAPEILLSVIEVCGHNFTAGDLGPSTIVGSAAFNMFIIIALCVYVVPDGETRKIKHLRVFFVTAAWSIFAYTWLYIILSVSSPGVVEVWEGLLTFFFFPICVVFAWVADRRLLFYKYVYKRYRAGKQRGMIIEHEGDRPASKTEIEMDGKVVNSHVDNFLDGALVLEVDERDQDDEEARREMARILKELKQKHPDKEIEQLIELANYQVLSQQQKSRAFYRIQATRLMTGAGNILKRHAADQARKAVSMHEVNMDVVENDPVSKVFFEQGTYQCLENCGTVALTIIRRGGDLTNTVFVDFRTEDGTANAGSDYEFTEGTVIFKPGETQKEIRVGIIDDDIFEEDENFLVHLSNVRVSSEVSEDGILDSNHVSAIACLGSPNTATITIFDDDHAGIFTFEEPVTHVSESIGIMEVKVLRTSGARGNVIIPYKTIEGTARGGGEDFEDTCGELEFQNDEIVKTISVKVIDDEEYEKNKTFFIEIGEPRLVEMSEKKALLLNELGGFTLTEGKKMYGQPVFRKVHARDHPIPSTVISISEEYDDKQPLTSKEEEERRIAEMGRPILGEHTKLEVIIEESYEFKSTVDKLIKKTNLALVVGTNSWREQFIEAITVSAGEDDDDDECGEEKLPSCFDYVMHFLTVFWKVLFAFVPPTEYWNGWACFIVSILMIGLLTAFIGDLASHFGCTIGLKDSVTAVVFVALGTSVPDTFASKVAATQDQYADASIGNVTGSNAVNVFLGIGVAWSIAAIYHAANGEQFKVSPGTLAFSVTLFTIFAFINVGVLLYRRRPEIGGELGGPRTAKLLTSSLFVLLWLLYIFFSSLEAYCHIKGF | ||||||
Glycosylation | 41 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 157 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Modified residue | 282 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 389 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Detected in heart, brain cortex and hippocampus (at protein level) (PubMed:16914199).
Cardiac sarcolemma or brain, and spleen. Expressed in all regions of the kidney, highest levels of expression in the distal convoluted tubule. Expressed throughout the CNS, in decreasing order of abundance in hippocampus, cortex, cerebellum, hypothalamus, midbrain and striatum. Expressed in numerous regions of the brain including multiple cortical layers, hippocampus, septal nuclei, thalamic nuclei, cerebellum, hypothalamus, olfactory bulb and brainstem. Also expressed in various regions of the spinal cord, ventricles and atria of the heart, lung, adrenals and kidney. Isoform 4 seems to be a predominant isoform in aorta, stomach, liver, and kidney.
Cardiac sarcolemma or brain, and spleen. Expressed in all regions of the kidney, highest levels of expression in the distal convoluted tubule. Expressed throughout the CNS, in decreasing order of abundance in hippocampus, cortex, cerebellum, hypothalamus, midbrain and striatum. Expressed in numerous regions of the brain including multiple cortical layers, hippocampus, septal nuclei, thalamic nuclei, cerebellum, hypothalamus, olfactory bulb and brainstem. Also expressed in various regions of the spinal cord, ventricles and atria of the heart, lung, adrenals and kidney. Isoform 4 seems to be a predominant isoform in aorta, stomach, liver, and kidney.
Gene expression databases
Structure
Family & Domains
Features
Showing features for repeat, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 138-178 | Alpha-1 | ||||
Sequence: ALGSSAPEILLSVIEVCGHNFTAGDLGPSTIVGSAAFNMFI | ||||||
Region | 251-270 | Putative calmodulin-binding region | ||||
Sequence: RRLLFYKYVYKRYRAGKQRG | ||||||
Domain | 393-493 | Calx-beta 1 | ||||
Sequence: VNMDVVENDPVSKVFFEQGTYQCLENCGTVALTIIRRGGDLTNTVFVDFRTEDGTANAGSDYEFTEGTVIFKPGETQKEIRVGIIDDDIFEEDENFLVHLS | ||||||
Domain | 524-624 | Calx-beta 2 | ||||
Sequence: ATITIFDDDHAGIFTFEEPVTHVSESIGIMEVKVLRTSGARGNVIIPYKTIEGTARGGGEDFEDTCGELEFQNDEIVKTISVKVIDDEEYEKNKTFFIEIG | ||||||
Repeat | 840-876 | Alpha-2 | ||||
Sequence: ALGTSVPDTFASKVAATQDQYADASIGNVTGSNAVNV |
Domain
The cytoplasmic Calx-beta domains bind the regulatory Ca2+. The first Calx-beta domain can bind up to four Ca2+ ions. The second domain can bind another two Ca2+ ions that are essential for calcium-regulated ion exchange.
Sequence similarities
Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 7 isoforms produced by Alternative splicing.
Q01728-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- SynonymsHeart, NaCa1
- Length971
- Mass (Da)108,185
- Last updated1996-02-01 v3
- ChecksumEC456CFE3AFC6A69
Q01728-2
- Name2
- SynonymsBrain-1, NaCa5
Q01728-3
- Name3
- SynonymsBrain-2, NaCa4
Q01728-4
- Name4
- SynonymsKidney-1, NaCa7, SNCX1
Q01728-5
- Name5
- SynonymsKidney-2, NaCa3, RNCX1
Q01728-6
- Name6
- SynonymsSDNCX1.10
Q01728-7
- Name7
Computationally mapped potential isoform sequences
There are 7 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
M0R3V7 | M0R3V7_RAT | Slc8a1 | 940 | ||
A0A8I6AXH4 | A0A8I6AXH4_RAT | Slc8a1 | 972 | ||
A0A8I5ZXH4 | A0A8I5ZXH4_RAT | Slc8a1 | 965 | ||
A0A8L2QNY8 | A0A8L2QNY8_RAT | Slc8a1 | 964 | ||
A0A8L2QKS1 | A0A8L2QKS1_RAT | Slc8a1 | 973 | ||
A0A8I5XVV7 | A0A8I5XVV7_RAT | Slc8a1 | 949 | ||
A0A8L2QK59 | A0A8L2QK59_RAT | Slc8a1 | 965 |
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 148 | in Ref. 5; AAD23388/AAD23389 and 6; AAK52307 | ||||
Sequence: L → P | ||||||
Sequence conflict | 218 | in Ref. 5; AAD23387 | ||||
Sequence: I → F | ||||||
Sequence conflict | 250 | in Ref. 1; CAA48273 | ||||
Sequence: D → A | ||||||
Sequence conflict | 402 | in Ref. 1; CAA48273 | ||||
Sequence: P → A | ||||||
Sequence conflict | 438 | in Ref. 5; AAD23386/AAD23387 | ||||
Sequence: F → S | ||||||
Sequence conflict | 500 | in Ref. 5; AAD23388 and 6; AAK52307 | ||||
Sequence: E → G | ||||||
Alternative sequence | VSP_003401 | 602-635 | in isoform 4, isoform 5, isoform 6 and isoform 7 | |||
Sequence: TISVKVIDDEEYEKNKTFFIEIGEPRLVEMSEKK → IITIRIFDREEYEKECSFSLVLEEPKWIRRGMK | ||||||
Alternative sequence | VSP_003402 | 636-642 | in isoform 2, isoform 3, isoform 4, isoform 5 and isoform 6 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_029836 | 649 | in isoform 6 and isoform 7 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_003403 | 649-654 | in isoform 2 and isoform 4 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_003404 | 649-677 | in isoform 3 and isoform 5 | |||
Sequence: Missing | ||||||
Sequence conflict | 789 | in Ref. 8 | ||||
Sequence: F → N | ||||||
Sequence conflict | 861 | in Ref. 8 | ||||
Sequence: A → R |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X68191 EMBL· GenBank· DDBJ | CAA48273.1 EMBL· GenBank· DDBJ | mRNA | ||
X68812 EMBL· GenBank· DDBJ | CAA48707.1 EMBL· GenBank· DDBJ | mRNA | ||
X68813 EMBL· GenBank· DDBJ | CAA48708.1 EMBL· GenBank· DDBJ | mRNA | ||
U04933 EMBL· GenBank· DDBJ | AAB39952.1 EMBL· GenBank· DDBJ | mRNA | ||
U04934 EMBL· GenBank· DDBJ | AAA19124.1 EMBL· GenBank· DDBJ | mRNA | ||
U04936 EMBL· GenBank· DDBJ | AAA19125.1 EMBL· GenBank· DDBJ | mRNA | ||
AF109163 EMBL· GenBank· DDBJ | AAD23386.1 EMBL· GenBank· DDBJ | mRNA | ||
AF109164 EMBL· GenBank· DDBJ | AAD23387.1 EMBL· GenBank· DDBJ | mRNA | ||
AF109165 EMBL· GenBank· DDBJ | AAD23388.1 EMBL· GenBank· DDBJ | mRNA | ||
AF109166 EMBL· GenBank· DDBJ | AAD23389.1 EMBL· GenBank· DDBJ | mRNA | ||
AY033398 EMBL· GenBank· DDBJ | AAK52307.1 EMBL· GenBank· DDBJ | mRNA |