Q01651 · G3P_CORGL
- ProteinGlyceraldehyde-3-phosphate dehydrogenase
- Genegap
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids334 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.
Catalytic activity
- D-glyceraldehyde 3-phosphate + phosphate + NAD+ = (2R)-3-phospho-glyceroyl phosphate + NADH + H+
Pathway
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 12-13 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 35 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 79 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 121 | NAD+ (UniProtKB | ChEBI) | |||
Binding site | 152-154 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Active site | 153 | Nucleophile | |||
Site | 180 | Activates thiol group during catalysis | |||
Binding site | 183 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 198 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 211-212 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 234 | D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI) | |||
Binding site | 315 | NAD+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity | |
Molecular Function | NAD binding | |
Molecular Function | NADP binding | |
Biological Process | glucose metabolic process | |
Biological Process | glycolytic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGlyceraldehyde-3-phosphate dehydrogenase
- EC number
- Short namesGAPDH
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Corynebacteriaceae > Corynebacterium
Accessions
- Primary accessionQ01651
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000145647 | 1-334 | Glyceraldehyde-3-phosphate dehydrogenase | ||
Interaction
Structure
Sequence
- Sequence statusComplete
- Length334
- Mass (Da)36,046
- Last updated2002-07-26 v2
- Checksum33792AF65FA90FA7
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 25-26 | in Ref. 1; CAA42045 | |||
Sequence conflict | 333-334 | in Ref. 1; CAA42045 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X59403 EMBL· GenBank· DDBJ | CAA42045.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BA000036 EMBL· GenBank· DDBJ | BAB98981.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX927152 EMBL· GenBank· DDBJ | CAF21596.1 EMBL· GenBank· DDBJ | Genomic DNA |