Q01651 · G3P_CORGL

Function

function

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.

Catalytic activity

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Features

Showing features for binding site, active site, site.

Type
IDPosition(s)Description
Binding site12-13NAD+ (UniProtKB | ChEBI)
Binding site35NAD+ (UniProtKB | ChEBI)
Binding site79NAD+ (UniProtKB | ChEBI)
Binding site121NAD+ (UniProtKB | ChEBI)
Binding site152-154D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Active site153Nucleophile
Site180Activates thiol group during catalysis
Binding site183D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site198D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site211-212D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site234D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site315NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Molecular FunctionNAD binding
Molecular FunctionNADP binding
Biological Processglucose metabolic process
Biological Processglycolytic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glyceraldehyde-3-phosphate dehydrogenase
  • EC number
  • Short names
    GAPDH
  • Alternative names
    • NAD-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

    • Name
      gap
    • Ordered locus names
      Cgl1588, cg1791

Organism names

Accessions

  • Primary accession
    Q01651

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001456471-334Glyceraldehyde-3-phosphate dehydrogenase

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    334
  • Mass (Da)
    36,046
  • Last updated
    2002-07-26 v2
  • Checksum
    33792AF65FA90FA7
MTIRVGINGFGRIGRNFFRAVLERSDDLEVVAVNDLTDNKTLSTLLKFDSIMGRLGQEVEYDDDSITVGGKRIAVYAERDPKNLDWAAHNVDIVIESTGFFTDANAAKAHIEAGAKKVIISAPASNEDATFVYGVNHESYDPENHNVISGASCTTNCLAPMAKVLNDKFGIENGLMTTVHAYTGDQRLHDAPHRDLRRARAAAVNIVPTSTGAAKAVALVLPELKGKLDGYALRVPVITGSATDLTFNTKSEVTVESINAAIKEAAVGEFGETLAYSEEPLVSTDIVHDSHGSIFDAGLTKVSGNTVKVVSWYDNEWGYTCQLLRLTELVASKL

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict25-26in Ref. 1; CAA42045
Sequence conflict333-334in Ref. 1; CAA42045

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X59403
EMBL· GenBank· DDBJ
CAA42045.1
EMBL· GenBank· DDBJ
Genomic DNA
BA000036
EMBL· GenBank· DDBJ
BAB98981.1
EMBL· GenBank· DDBJ
Genomic DNA
BX927152
EMBL· GenBank· DDBJ
CAF21596.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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