Q015G2 · SYAP_OSTTA
- ProteinProbable alanine--tRNA ligase, chloroplastic/mitochondrial
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids976 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.
Catalytic activity
- tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate
Cofactor
Note: Binds 1 zinc ion per subunit.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | cytosol | |
Cellular Component | mitochondrion | |
Molecular Function | alanine-tRNA ligase activity | |
Molecular Function | aminoacyl-tRNA editing activity | |
Molecular Function | ATP binding | |
Molecular Function | tRNA binding | |
Molecular Function | zinc ion binding | |
Biological Process | alanyl-tRNA aminoacylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable alanine--tRNA ligase, chloroplastic/mitochondrial
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Chlorophyta > Mamiellophyceae > Mamiellales > Bathycoccaceae > Ostreococcus
Accessions
- Primary accessionQ015G2
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-54 | Chloroplast and mitochondrion | ||||
Sequence: MPRPGFAHATAPALAHARARISPVARRRVVVMRTRVDGAAKSLVTQLRLALGST | ||||||
Chain | PRO_0000402308 | 55-976 | Probable alanine--tRNA ligase, chloroplastic/mitochondrial | |||
Sequence: ASRASSIASARRRVRALGTATNDQSTGTRANPNAEGKDNSGRGIRRRFLEFYEARGHSRQPSASLVPEDPTVLLTIAGMLQFKPVFMGQREREMPRATTTQKCVRTNDIENVGVTARHHTFFEMLGNFSFGDYFKREACEWAWELATNEFGLNPERVWVSVFREDDEAFAIWRDVVGVPESRIKRMDEKDNFWAAGPTGPCGPCSELYYDFHPERGLDGADLDDDSRFIEFYNLVFMELNRDADGGIKPLKNKNIDTGMGLERMAQILQGVSNNYETDLIRPIIDKAASMAGLDYASCSATQKQQLKVIGDHTRAVTYMISDGVFASNIGRGYIVRRLLRRVVRNGRLLGIKPADGQSAFTPSIAEVAISMSEECDPQVVKNTARILAELEREELSFQKTLGRGEEMLAELIEKAKDSKSGLSGKDAFTLYDTYGFPLDITTDVASEAGIAVDLEGFEKAMAEQRSMSQAAHQTVDVTAGNALAQVADELGAMSEFIGYDNISSDVSNVLAIVSGGESVEEASGGARVDVVLDVTPFYAESGGQVGDNGFLHSADGAVLKVTDVQKAGGGRIIVHSATVVKGSIKKGSQVSANVDENARRRARNNHTATHLLQSALKKVLGDDVSQAGSLCGFDRLRFDFNCPKAVTEAQLLEVETLVNGWISQSADLTAEEMPIAAAKEKGATMMFGEKYGDVVRVVDVPGISMELCGGTHVSNTAEIGGFKIISEAGIASGIRRIEAVAGAGVVELLQQRDAVVKQLASTLRVPPEEIASRVSGMQKDLVAAQKLAESLRGELAVAKANALVSEARAVGESKVLVARLDGVDPGALKVAAENLATQLGDGAAVILGSANGDNVGLVALFDTKVQKDGDLKAGQVLGAAAKRCGGGGGGKPGFAQAGGRDATQLDAALDEALQTLTTALDK |
Interaction
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 71-95 | Disordered | ||||
Sequence: LGTATNDQSTGTRANPNAEGKDNSG | ||||||
Compositional bias | 72-89 | Polar residues | ||||
Sequence: GTATNDQSTGTRANPNAE |
Domain
Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length976
- Mass (Da)104,285
- Last updated2015-10-14 v2
- Checksum1DC125E90AD04A01
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 72-89 | Polar residues | ||||
Sequence: GTATNDQSTGTRANPNAE |
Keywords
- Technical term