Q00946 · VPRT_ASFB7
- ProteinCysteine protease S273R
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids273 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Cysteine protease that plays several role during infection including processing of the structural polyprotein or inhibition of the host immune response. Catalyzes the maturation of the pp220 and pp62 polyprotein precursors into core-shell proteins (PubMed:12438573).
Plays a role in the disruption of host pyroptosis via specific cleavage of gasdermin D/GSDMD (PubMed:34890644).
In addition, strongly decreases the host cGAS-STING signaling by targeting IKBKE via its enzymatic activity (PubMed:35437104).
Impairs also host FOXJ1-mediated antiviral effect via degradation of FOXJ1 (PubMed:35513267).
Cleaves host G3BP1 inducing loss of stress granules formation (PubMed:37209818).
Interacts with and induces the degradation of host STAT2 via polyubiquitination of the latter (PubMed:36856422).
Plays a role in the disruption of host pyroptosis via specific cleavage of gasdermin D/GSDMD (PubMed:34890644).
In addition, strongly decreases the host cGAS-STING signaling by targeting IKBKE via its enzymatic activity (PubMed:35437104).
Impairs also host FOXJ1-mediated antiviral effect via degradation of FOXJ1 (PubMed:35513267).
Cleaves host G3BP1 inducing loss of stress granules formation (PubMed:37209818).
Interacts with and induces the degradation of host STAT2 via polyubiquitination of the latter (PubMed:36856422).
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 168 | |||||
Sequence: H | ||||||
Active site | 187 | |||||
Sequence: N | ||||||
Binding site | 226 | substrate | ||||
Sequence: Q | ||||||
Active site | 232 | Nucleophile | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell cytoplasm | |
Cellular Component | virion component | |
Molecular Function | cysteine-type endopeptidase activity | |
Biological Process | proteolysis | |
Biological Process | symbiont-mediated suppression of host innate immune response | |
Biological Process | viral protein processing | |
Biological Process | virus-mediated perturbation of host defense response |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameCysteine protease S273R
- EC number
- Short namespS273R
Gene names
Organism names
- Taxonomic lineageViruses > Varidnaviria > Bamfordvirae > Nucleocytoviricota > Pokkesviricetes > Asfuvirales > Asfarviridae > Asfivirus > African swine fever virus
- Virus hosts
Accessions
- Primary accessionQ00946
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Found in the perinuclear cytoplasmic viral factories during assembly.
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 168 | Loss of activity. | ||||
Sequence: H → R | ||||||
Mutagenesis | 232 | Loss of activity. | ||||
Sequence: C → S |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000101738 | 1-273 | Cysteine protease S273R | |||
Sequence: MSILEKITSSPSECAEHLTNKDSCLSKKIQKELTSFLEKKETLGCDSESCVITHPAVKAYAQQKGLDLSKELETRFKAPGPRNNTGLLTNFNIDETLQRWAIKYTKFFNCPFSMMDFERVHYKFNQVDMVKVYKGEELQYVEGKVVKRPCNTFGCVLNTDFSTGTGKHWVAIFVDMRGDCWSIEYFNSAGNSPPGPVIRWMERVKQQLLKIHHTVKTLAVTNIRHQRSQTECGPYSLFYIRARLDNVSYAHFISARITDEDMYKFRTHLFRIA |
Expression
Induction
Expressed in the late phase of the viral replicative cycle.
Keywords
- Developmental stage
Sequence
- Sequence statusComplete
- Length273
- Mass (Da)31,550
- Last updated1993-04-01 v1
- ChecksumC5AA778179C57C17
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
M88699 EMBL· GenBank· DDBJ | AAA42734.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
U18466 EMBL· GenBank· DDBJ | AAA65340.1 EMBL· GenBank· DDBJ | Genomic DNA |