P9WPQ4 · BIOD_MYCTO
- ProteinATP-dependent dethiobiotin synthetase BioD
- GenebioD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids226 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.
Catalytic activity
- (7R,8S)-7,8-diammoniononanoate + CO2 + ATP = (4R,5S)-dethiobiotin + ADP + phosphate + 3 H+
Cofactor
Pathway
Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 12-17 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GVGKTV | ||||||
Binding site | 16 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Active site | 37 | |||||
Sequence: K | ||||||
Binding site | 41 | substrate | ||||
Sequence: T | ||||||
Binding site | 49 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 49 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 108 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 108-111 | ATP (UniProtKB | ChEBI) | ||||
Sequence: EGAG | ||||||
Binding site | 169-170 | ATP (UniProtKB | ChEBI) | ||||
Sequence: GS | ||||||
Binding site | 197-199 | ATP (UniProtKB | ChEBI) | ||||
Sequence: PAG |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | ATP binding | |
Molecular Function | dethiobiotin synthase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | biotin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent dethiobiotin synthetase BioD
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionP9WPQ4
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000426911 | 1-226 | ATP-dependent dethiobiotin synthetase BioD | |||
Sequence: MTILVVTGTGTGVGKTVVCAALASAARQAGIDVAVCKPVQTGTARGDDDLAEVGRLAGVTQLAGLARYPQPMAPAAAAEHAGMALPARDQIVRLIADLDRPGRLTLVEGAGGLLVELAEPGVTLRDVAVDVAAAALVVVTADLGTLNHTKLTLEALAAQQVSCAGLVIGSWPDPPGLVAASNRSALARIATVRAALPAGAASLDAGDFAAMSAAAFDRNWVAGLVG |
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length226
- Mass (Da)22,426
- Last updated2014-04-16 v1
- Checksum43581D116CB02AAE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE000516 EMBL· GenBank· DDBJ | AAK45888.1 EMBL· GenBank· DDBJ | Genomic DNA |