P9WNT3 · DPO41_MYCTU
- ProteinDNA polymerase IV 1
- GenedinB1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids468 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).
Catalytic activity
- a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)
Cofactor
Note: Binds 2 magnesium ions per subunit.
Features
Showing features for binding site, site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | plasma membrane | |
Molecular Function | damaged DNA binding | |
Molecular Function | DNA-directed DNA polymerase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | DNA-templated DNA replication | |
Biological Process | error-prone translesion synthesis | |
Biological Process | SOS response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDNA polymerase IV 1
- EC number
- Short namesPol IV 1
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionP9WNT3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000173924 | 1-468 | DNA polymerase IV 1 | |||
Sequence: MESRWVLHLDMDAFFASVEQLTRPTLRGRPVLVGGLGGRGVVAGASYEARAYGARSAMPMHQARRLIGVTAVVLPPRGVVYGIASRRVFDTVRGLVPVVEQLSFDEAFAEPPQLAGAVAEDVETFCERLRRRVRDETGLIASVGAGSGKQIAKIASGLAKPDGIRVVRHAEEQALLSGLPVRRLWGIGPVAEEKLHRLGIETIGQLAALSDAEAANILGATIGPALHRLARGIDDRPVVERAEAKQISAESTFAVDLTTMEQLHEAIDSIAEHAHQRLLRDGRGARTITVKLKKSDMSTLTRSATMPYPTTDAGALFTVARRLLPDPLQIGPIRLLGVGFSGLSDIRQESLFADSDLTQETAAAHYVETPGAVVPAAHDATMWRVGDDVAHPELGHGWVQGAGHGVVTVRFETRGSGPGSARTFPVDTGDISNASPLDSLDWPDYIGQLSVEGSAGASAPTVDDVGDR |
Proteomic databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 6-188 | UmuC | ||||
Sequence: VLHLDMDAFFASVEQLTRPTLRGRPVLVGGLGGRGVVAGASYEARAYGARSAMPMHQARRLIGVTAVVLPPRGVVYGIASRRVFDTVRGLVPVVEQLSFDEAFAEPPQLAGAVAEDVETFCERLRRRVRDETGLIASVGAGSGKQIAKIASGLAKPDGIRVVRHAEEQALLSGLPVRRLWGIG |
Sequence similarities
Belongs to the DNA polymerase type-Y family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length468
- Mass (Da)49,765
- Last updated2014-04-16 v1
- ChecksumF6CF59BC5FAEA1D8
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL123456 EMBL· GenBank· DDBJ | CCP44301.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |