P9WNP5 · MENB_MYCTU
- Protein1,4-dihydroxy-2-naphthoyl-CoA synthase
- GenemenB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids314 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Converts o-succinylbenzoyl-CoA (OSB-CoA) to 1,4-dihydroxy-2-naphthoyl-CoA (DHNA-CoA).
Catalytic activity
- 2-succinylbenzoyl-CoA + H+ = 1,4-dihydroxy-2-naphthoyl-CoA + H2O
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
7.3 μM | O-succinylbenzoyl-CoA | 7.0 | 25 | |||
22.4 μM | O-succinylbenzoyl-CoA | 7.0 | 25 |
kcat is 14.9 min-1 with O-succinylbenzoyl-CoA at 25 degrees Celsius and pH 7.0 (PubMed:12909628).
kcat is 4620 sec-1 with O-succinylbenzoyl-CoA at 25 degrees Celsius and pH 7.0 (PubMed:21830810).
kcat is 4620 sec-1 with O-succinylbenzoyl-CoA at 25 degrees Celsius and pH 7.0 (PubMed:21830810).
Pathway
Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 6/7.
Quinol/quinone metabolism; menaquinone biosynthesis.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 58 | substrate; ligand shared between two neighboring subunits; in other chain | ||||
Sequence: R | ||||||
Binding site | 95 | substrate; ligand shared between two neighboring subunits; in other chain | ||||
Sequence: K | ||||||
Binding site | 103-107 | substrate; ligand shared between two neighboring subunits; in other chain | ||||
Sequence: SGGDQ | ||||||
Binding site | 115 | substrate; ligand shared between two neighboring subunits; in other chain | ||||
Sequence: Y | ||||||
Site | 115 | Important for catalysis | ||||
Sequence: Y | ||||||
Binding site | 157-161 | substrate; ligand shared between two neighboring subunits; in other chain | ||||
Sequence: WAAGG | ||||||
Binding site | 184 | substrate; ligand shared between two neighboring subunits; in other chain | ||||
Sequence: T | ||||||
Site | 185 | Important for catalysis | ||||
Sequence: D | ||||||
Binding site | 190 | substrate; ligand shared between two neighboring subunits; in other chain | ||||
Sequence: S | ||||||
Binding site | 287 | substrate; ligand shared between two neighboring subunits | ||||
Sequence: Y | ||||||
Site | 287 | Important for catalysis | ||||
Sequence: Y | ||||||
Binding site | 302 | substrate; ligand shared between two neighboring subunits | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | 1,4-dihydroxy-2-naphthoyl-CoA synthase activity | |
Biological Process | menaquinone biosynthetic process | |
Biological Process | protein hexamerization |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name1,4-dihydroxy-2-naphthoyl-CoA synthase
- EC number
- Short namesDHNA-CoA synthase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionP9WNP5
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 133 | Loss of DHNA-CoA synthase activity. | ||||
Sequence: R → A | ||||||
Mutagenesis | 185 | Nearly abolishes DHNA-CoA synthase activity. | ||||
Sequence: D → E | ||||||
Mutagenesis | 185 | Loss of DHNA-CoA synthase activity. | ||||
Sequence: D → G or N | ||||||
Mutagenesis | 190 | Reduces affinity for substrate. Nearly abolishes DHNA-CoA synthase activity. | ||||
Sequence: S → A | ||||||
Mutagenesis | 192 | Loss of DHNA-CoA synthase activity. | ||||
Sequence: D → N | ||||||
Mutagenesis | 287 | Loss of DHNA-CoA synthase activity. | ||||
Sequence: Y → F |
Chemistry
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000399471 | 1-314 | 1,4-dihydroxy-2-naphthoyl-CoA synthase | |||
Sequence: MVAPAGEQGRSSTALSDNPFDAKAWRLVDGFDDLTDITYHRHVDDATVRVAFNRPEVRNAFRPHTVDELYRVLDHARMSPDVGVVLLTGNGPSPKDGGWAFCSGGDQRIRGRSGYQYASGDTADTVDVARAGRLHILEVQRLIRFMPKVVICLVNGWAAGGGHSLHVVCDLTLASREYARFKQTDADVGSFDGGYGSAYLARQVGQKFAREIFFLGRTYTAEQMHQMGAVNAVAEHAELETVGLQWAAEINAKSPQAQRMLKFAFNLLDDGLVGQQLFAGEATRLAYMTDEAVEGRDAFLQKRPPDWSPFPRYF |
Proteomic databases
Structure
Family & Domains
Sequence similarities
Belongs to the enoyl-CoA hydratase/isomerase family. MenB subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length314
- Mass (Da)34,689
- Last updated2014-04-16 v1
- ChecksumAF2D192A9F607DC7
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL123456 EMBL· GenBank· DDBJ | CCP43286.1 EMBL· GenBank· DDBJ | Genomic DNA |