P9WNE3 · CPFC_MYCTU

Function

function

Involved in coproporphyrin-dependent heme b biosynthesis (PubMed:25646457).
Catalyzes the insertion of ferrous iron into coproporphyrin III to form Fe-coproporphyrin III (PubMed:25646457).
Has weaker activity with coproporphyrin I, protoporphyrin IX, deuteroporphyrin, 2,4 hydroxyethyl and 2,4 disulfonate (PubMed:11948160, PubMed:25646457).

Miscellaneous

Was identified as a high-confidence drug target.

Catalytic activity

Cofactor

[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

Note: Binds 1 [2Fe-2S] cluster.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
10.5 μMcoproporphyrin III
720 μMprotoporphyrin IX
kcat is 1.8 min-1 with coproporphyrin III as substrate. kcat is 0.8 min-1 with protoporphyrin IX as substrate.

Pathway

Porphyrin-containing compound metabolism; protoheme biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site52Fe-coproporphyrin III (UniProtKB | ChEBI)
Binding site113[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site116Fe-coproporphyrin III (UniProtKB | ChEBI)
Binding site172Fe2+ (UniProtKB | ChEBI)
Binding site255Fe2+ (UniProtKB | ChEBI)
Binding site316[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site325[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site330[2Fe-2S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentplasma membrane
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Functionferrochelatase activity
Molecular Functionmetal ion binding
Biological Processheme biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Coproporphyrin III ferrochelatase
  • EC number

Gene names

    • Name
      cpfC
    • Synonyms
      hemH
      , hemZ
    • ORF names
      MTCY277.06
    • Ordered locus names
      Rv1485

Organism names

Accessions

  • Primary accession
    P9WNE3
  • Secondary accessions
    • L0T8E3
    • P0A576
    • P71765

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001751671-344Coproporphyrin III ferrochelatase

Proteomic databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the ferrochelatase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    344
  • Mass (Da)
    37,144
  • Last updated
    2014-04-16 v1
  • Checksum
    17A5023246B670B6
MQFDAVLLLSFGGPEGPEQVRPFLENVTRGRGVPAERLDAVAEHYLHFGGVSPINGINRTLIAELEAQQELPVYFGNRNWEPYVEDAVTAMRDNGVRRAAVFATSAWSGYSSCTQYVEDIARARRAAGRDAPELVKLRPYFDHPLFVEMFADAITAAAATVRGDARLVFTAHSIPTAADRRCGPNLYSRQVAYATRLVAAAAGYCDFDLAWQSRSGPPQVPWLEPDVTDQLTGLAGAGINAVIVCPIGFVADHIEVVWDLDHELRLQAEAAGIAYARASTPNADPRFARLARGLIDELRYGRIPARVSGPDPVPGCLSSINGQPCRPPHCVASVSPARPSAGSP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP44245.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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