P9WND7 · MFTD_MYCTU
- ProteinPre-mycofactocin synthase
- GenemftD
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids396 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Involved in the biosynthesis of the enzyme cofactor mycofactocin (MFT). Catalyzes the oxidative deamination of AHDP (3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidin-2-one), forming an alpha-keto amide moiety on the resulting molecule, which is called pre-mycofactocin (PMFT). This reaction occurs via a 5-[(4-hydroxyphenyl)methyl]-3-imino-4,4-dimethylpyrrolidin-2-one intermediate, which converts to PMFT. The alpha-keto amide moiety is the redox-active center for the redox activity of mycofactocin.
Catalytic activity
- 3-amino-5-[(4-hydroxyphenyl)methyl]-4,4-dimethyl-2-pyrrolidin-2-one + O2 + H2O = pre-mycofactocin + H2O2 + NH4+
Cofactor
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 108 | FMN (UniProtKB | ChEBI) | |||
Binding site | 128 | FMN (UniProtKB | ChEBI) | |||
Binding site | 156 | FMN (UniProtKB | ChEBI) | |||
Binding site | 254 | FMN (UniProtKB | ChEBI) | |||
Active site | 278 | Proton acceptor | |||
Binding site | 309-313 | FMN (UniProtKB | ChEBI) | |||
Binding site | 332-333 | FMN (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | FMN binding | |
Molecular Function | oxidoreductase activity | |
Biological Process | mycofactocin biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePre-mycofactocin synthase
- EC number
- Short namesPMFT synthase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionP9WND7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000390892 | 1-396 | Pre-mycofactocin synthase | ||
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 1-383 | FMN hydroxy acid dehydrogenase | |||
Sequence similarities
Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length396
- Mass (Da)42,161
- Last updated2014-04-16 v1
- MD5 Checksum46FB428D24D20EC519EE7EB829C66812
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL123456 EMBL· GenBank· DDBJ | CCP43438.1 EMBL· GenBank· DDBJ | Genomic DNA |