P9WMY3 · WBBL_MYCTU
- ProteinN-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase
- GenewbbL
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids307 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the biosynthesis of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the transfer of the rhamnosyl moiety from dTDP-rhamnosyl (dTDP-Rha) onto the decaprenyl-pyrophosphoryl-GlcNAc (C50-PP-GlcNAc), yielding rhamnosyl-decaprenyl-pyrophosphoryl-GlcNAc (Rha-C50-PP-GlcNAc).
Miscellaneous
Was identified as a high-confidence drug target.
Catalytic activity
- N-acetyl-alpha-D-glucosaminyl-1-diphospho-trans,octa-cis-decaprenol + dTDP-beta-L-rhamnose = alpha-L-rhamnosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octa-cis-decaprenol + dTDP + H+
Cofactor
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
18 μM | N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | |||||
35 μM | dTDP-6-deoxy-beta-L-mannose |
pH Dependence
Optimum pH is 8.6.
Temperature Dependence
Optimum temperature is 30 degrees Celsius.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | decaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate:dTDP-alpha-L-rhamnose rhamnosyltransferase activity | |
Molecular Function | hexosyltransferase activity | |
Biological Process | extracellular polysaccharide biosynthetic process |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameN-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionP9WMY3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene lead to irreversible loss of viability.
PTM/Processing
Features
Showing features for initiator methionine, chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Initiator methionine | 1 | Removed | |||
Chain | PRO_0000395354 | 2-307 | N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase | ||
Proteomic databases
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length307
- Mass (Da)33,920
- Last updated2016-11-02 v2
- Checksum6C3621E158BB096D
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL123456 EMBL· GenBank· DDBJ | CCP46084.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |