P9WMY3 · WBBL_MYCTU

Function

function

Involved in the biosynthesis of the mycolylarabinogalactan-peptidoglycan (mAGP) complex, an essential component of the mycobacterial cell wall. Catalyzes the transfer of the rhamnosyl moiety from dTDP-rhamnosyl (dTDP-Rha) onto the decaprenyl-pyrophosphoryl-GlcNAc (C50-PP-GlcNAc), yielding rhamnosyl-decaprenyl-pyrophosphoryl-GlcNAc (Rha-C50-PP-GlcNAc).

Miscellaneous

Was identified as a high-confidence drug target.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
18 μMN-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
35 μMdTDP-6-deoxy-beta-L-mannose

pH Dependence

Optimum pH is 8.6.

Temperature Dependence

Optimum temperature is 30 degrees Celsius.

GO annotations

AspectTerm
Cellular Componentplasma membrane
Molecular Functiondecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate:dTDP-alpha-L-rhamnose rhamnosyltransferase activity
Molecular Functionhexosyltransferase activity
Biological Processextracellular polysaccharide biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase
  • EC number
  • Alternative names
    • Rhamnosyltransferase WbbL
    • dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase

Gene names

    • Name
      wbbL
    • Synonyms
      wbbL1
    • Ordered locus names
      Rv3265c

Organism names

Accessions

  • Primary accession
    P9WMY3
  • Secondary accessions
    • L0TF42
    • Q6MWZ0
    • Q7D5T2

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene lead to irreversible loss of viability.

PTM/Processing

Features

Showing features for initiator methionine, chain.

Type
IDPosition(s)Description
Initiator methionine1Removed
ChainPRO_00003953542-307N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase

Proteomic databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the glycosyltransferase 2 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    307
  • Mass (Da)
    33,920
  • Last updated
    2016-11-02 v2
  • Checksum
    6C3621E158BB096D
MTDVLPVVAVTYSPGPHLERFLASLSLATERPVSVLLADNGSTDGTPQAAVQRYPNVRLLPTGANLGYGTAVNRTIAQLGEMAGDAGEPWVDDWVIVANPDVQWGPGSIDALLDAASRWPRAGALGPLIRDPDGSVYPSARQMPSLIRGGMHAVLGPFWPRNPWTTAYRQERLEPSERPVGWLSGSCLLVRRSAFGQVGGFDERYFMYMEDVDLGDRLGKAGWLSVYVPSAEVLHHKAHSTGRDPASHLAAHHKSTYIFLADRHSGWWRAPLRWTLRGSLALRSHLMVRSSLRRSRRRKLKLVEGRH

Sequence caution

The sequence CCP46084.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP46084.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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