P9WMQ1 · UVRD1_MYCTU

Function

function

DNA-dependent ATPase, acting on dsDNA with a 3'-ssDNA tail, unwinding with 3'-to 5'-polarity. A minimal tail of 18 nt is required for activity. Also highly efficient on nicked DNA. Involved in the post-incision events of nucleotide excision repair, as well as in nitrosative and oxidative stress response and possibly in persistence in the host. Inhibits RecA-mediated DNA strand exchange; this does not require ATPase activity. When combined with UvrA greatly inhibits RecA-mediated DNA strand exchange.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Cu2+ (UniProtKB | Rhea| CHEBI:29036 )

Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Co2+ (UniProtKB | Rhea| CHEBI:48828 )

Note: Mg2+; Mn2+, Cu2+, Ni2+ or Co2+ also support ATPase activity.

Activity regulation

Inhibited by EDTA.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
60.2 μMATP
kcat is 43 sec-1 with ATP.

pH Dependence

Optimum pH is 7.5.

Temperature Dependence

Optimum temperature is 37 degrees Celsius.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site45-50ATP (UniProtKB | ChEBI)
Binding site309ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular ComponentDNA helicase complex
Cellular Componentpeptidoglycan-based cell wall
Cellular Componentplasma membrane
Molecular Function3'-5' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionATP-dependent activity, acting on DNA
Molecular FunctiondATP binding
Molecular FunctionDNA binding
Molecular Functionisomerase activity
Molecular Functionmagnesium ion binding
Biological ProcessDNA unwinding involved in DNA replication
Biological Processdouble-strand break repair
Biological Processnegative regulation of strand invasion
Biological Processrecombinational repair
Biological ProcessUV protection

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    ATP-dependent DNA helicase UvrD1
  • EC number
  • Alternative names
    • DNA 3'-5' helicase UvrD1

Gene names

    • Name
      uvrD1
    • Synonyms
      ivrd, pcrA
    • ORF names
      MTCY10D7.25c
    • Ordered locus names
      Rv0949

Organism names

Accessions

  • Primary accession
    P9WMQ1
  • Secondary accessions
    • L0T870
    • P0A5A3
    • P71561

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Forms small colonies, liquid growth is unchanged. Increased sensitivity to UV light, mitomycin C, nitrosative and oxidative stress; a double uvrA/uvrD1 mutant is even more sensitive. Single uvrD1 mutant is strongly attenuated in late stages of mouse infection, the double uvrA/uvrD1 mutant is strongly attenuated at all stages of infection.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis276Loss of ATPase and DNA unwinding, partially inhibits DNA strand exchange.

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylserine
ChainPRO_00001020552-771ATP-dependent DNA helicase UvrD1

Keywords

Proteomic databases

PTM databases

Expression

Induction

Up-regulated during mouse infection.

Interaction

Subunit

Monomer. Interacts with RecA.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-21Disordered
Domain21-311UvrD-like helicase ATP-binding
Domain312-603UvrD-like helicase C-terminal
Region691-716Disordered

Sequence similarities

Belongs to the helicase family. UvrD subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    771
  • Mass (Da)
    85,050
  • Last updated
    2014-04-16 v1
  • Checksum
    DBAA84E151F4E2C9
MSVHATDAKPPGPSPADQLLDGLNPQQRQAVVHEGSPLLIVAGAGSGKTAVLTRRIAYLMAARGVGVGQILAITFTNKAAAEMRERVVGLVGEKARYMWVSTFHSTCVRILRNQAALIEGLNSNFSIYDADDSRRLLQMVGRDLGLDIKRYSPRLLANAISNLKNELIDPHQALAGLTEDSDDLARAVASVYDEYQRRLRAANALDFDDLIGETVAVLQAFPQIAQYYRRRFRHVLVDEYQDTNHAQYVLVRELVGRDSNDGIPPGELCVVGDADQSIYAFRGATIRNIEDFERDYPDTRTILLEQNYRSTQNILSAANSVIARNAGRREKRLWTDAGAGELIVGYVADNEHDEARFVAEEIDALAEGSEITYNDVAVFYRTNNSSRSLEEVLIRAGIPYKVVGGVRFYERKEIRDIVAYLRVLDNPGDAVSLRRILNTPRRGIGDRAEACVAVYAENTGVGFGDALVAAAQGKVPMLNTRAEKAIAGFVEMFDELRGRLDDDLGELVEAVLERTGYRRELEASTDPQELARLDNLNELVSVAHEFSTDRENAAALGPDDEDVPDTGVLADFLERVSLVADADEIPEHGAGVVTLMTLHTAKGLEFPVVFVTGWEDGMFPHMRALDNPTELSEERRLAYVGITRARQRLYVSRAIVRSSWGQPMLNPESRFLREIPQELIDWRRTAPKPSFSAPVSGAGRFGSARPSPTRSGASRRPLLVLQVGDRVTHDKYGLGRVEEVSGVGESAMSLIDFGSSGRVKLMHNHAPVTKL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP43697.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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