P9WKD1 · PBPA_MYCTU

Function

function

Transpeptidase that catalyzes cross-linking of the peptidoglycan cell wall (Probable). Required for the regulation of cell length. Plays critical roles for the survival of the pathogen inside the host. Required for both bacterial survival and formation of granuloma structures in a guinea pig infection model (PubMed:29530985).

Caution

An article reported the phosphorylation of PbpA by PknB, but this paper was later retracted as some figures were modified prior to publication.

Catalytic activity

  • Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
    EC:3.4.16.4 (UniProtKB | ENZYME | Rhea)

Activity regulation

Inhibited by the antibiotics imipenem, penicillin G, and ceftriaxone.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site222Acyl-ester intermediate

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentplasma membrane
Molecular Functionpenicillin binding
Molecular Functionpeptidoglycan L,D-transpeptidase activity
Molecular Functionserine-type D-Ala-D-Ala carboxypeptidase activity
Biological Processcell wall organization
Biological Processpeptidoglycan biosynthetic process
Biological Processregulation of cell shape

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Peptidoglycan D,D-transpeptidase PbpA
  • EC number
  • Alternative names
    • Penicillin-binding protein A
      (PBPA
      )

Gene names

    • Name
      pbpA
    • ORF names
      MTCY10H4.16c
    • Ordered locus names
      Rv0016c

Organism names

Accessions

  • Primary accession
    P9WKD1
  • Secondary accessions
    • L0T427
    • P71586
    • Q7DAK5

Proteomes

Organism-specific databases

Subcellular Location

Cell inner membrane
; Single-pass type II membrane protein

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-7Cytoplasmic
Transmembrane8-28Helical; Signal-anchor for type II membrane protein
Topological domain29-491Periplasmic

Keywords

Phenotypes & Variants

Disruption phenotype

Deletion of the gene does not impact in vitro growth, but it leads to aberrations in the cell length. Deletion mutant shows much higher sensitivity to oxacillin and clavulanic acid. Mutant shows compromised bacterial virulence in the host.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis281Can rescue the deletion mutant.
Mutagenesis424Cannot rescue the deletion mutant.

Miscellaneous

PTM/Processing

Features

Showing features for chain, disulfide bond.

TypeIDPosition(s)Description
ChainPRO_00003438321-491Peptidoglycan D,D-transpeptidase PbpA
Disulfide bond266↔282

Keywords

Proteomic databases

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region160-484Transpeptidase

Domain

The apo form can adopt multiple conformations (PubMed:22365933).
The beta5-alpha11 loop near the active site is a flexible region that can adopt a variety of conformations in the acylated state of PBPA and appears important for acylation (PubMed:22365933).

Sequence similarities

Belongs to the transpeptidase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    491
  • Mass (Da)
    51,576
  • Last updated
    2014-04-16 v1
  • Checksum
    888DBA26BE251581
MNASLRRISVTVMALIVLLLLNATMTQVFTADGLRADPRNQRVLLDEYSRQRGQITAGGQLLAYSVATDGRFRFLRVYPNPEVYAPVTGFYSLRYSSTALERAEDPILNGSDRRLFGRRLADFFTGRDPRGGNVDTTINPRIQQAGWDAMQQGCYGPCKGAVVALEPSTGKILALVSSPSYDPNLLASHNPEVQAQAWQRLGDNPASPLTNRAISETYPPGSTFKVITTAAALAAGATETEQLTAAPTIPLPGSTAQLENYGGAPCGDEPTVSLREAFVKSCNTAFVQLGIRTGADALRSMARAFGLDSPPRPTPLQVAESTVGPIPDSAALGMTSIGQKDVALTPLANAEIAATIANGGITMRPYLVGSLKGPDLANISTTVGYQQRRAVSPQVAAKLTELMVGAEKVAQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPAQAPKVAVAVLVENGADRLSATGGALAAPIGRAVIEAALQGEP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP42738.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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