P9WGM9 · MPRA_MYCTU

Function

function

Member of the two-component regulatory system MprB/MprA which contributes to maintaining a balance among several systems involved in stress resistance and is required for establishment and maintenance of persistent infection in the host. Functions as a transcriptional regulator that recognizes a 19-bp nucleotide motif comprizing two loosely conserved 8-bp direct DNA-binding motif repeats separated by a 3-bp spacer region. MprB/MprA is involved in regulation of numerous stress-responsive genes, including up-regulation of two sigma factors, sigE and sigB as well as pepD and mprA, and repression of multiple genes from regulons associated with hypoxia, starvation and iron metabolism. The majority of genes regulated by MprB/MprA under a particular stress condition are different from those induced during normal growth, but several genes are commonly regulated under more than one condition.

Miscellaneous

Phosphorylation is not required for binding to DNA in vitro. However, phosphorylation enhances DNA binding and is required for activity in vivo.

Features

Showing features for dna binding.

123020406080100120140160180200220
TypeIDPosition(s)Description
DNA binding129-227OmpR/PhoB-type

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentpeptidoglycan-based cell wall
Cellular Componentplasma membrane
Cellular Componentprotein-DNA complex
Molecular FunctionDNA binding
Molecular Functionphosphorelay response regulator activity
Molecular Functiontranscription cis-regulatory region binding
Biological Processnegative regulation of DNA-templated transcription
Biological Processphosphorelay signal transduction system
Biological Processpositive regulation of DNA-templated transcription
Biological Processregulation of DNA-templated transcription
Biological Processresponse to alkaline pH

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Response regulator MprA
  • Alternative names
    • Mycobacterial persistence regulator A

Gene names

    • Name
      mprA
    • Ordered locus names
      Rv0981

Organism names

Accessions

  • Primary accession
    P9WGM9
  • Secondary accessions
    • L0T5H1
    • O53894
    • Q7D914

Proteomes

Organism-specific databases

Subcellular Location

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis48Abolishes phosphorylation. No loss of DNA-binding activity in vitro.
Mutagenesis53No loss of phosphorylation.

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00003084271-230Response regulator MprA
Modified residue484-aspartylphosphate

Post-translational modification

Phosphorylated and dephosphorylated by MprB.

Keywords

Proteomic databases

Expression

Induction

Autoregulated. Differentially up-regulated under different stress conditions, such as low concentrations of detergents and alkaline pH. Induced by low concentrations of sodium dodecyl sulfate (SDS) in a SigE-dependent manner. In strain ATCC 25618 / H37Rv, repressed during growth in macrophages.

Interaction

Subunit

Monomer. Interaction with each conserved 8-bp repeat requires tandem binding by two protein monomers (Probable).

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain4-118Response regulatory

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    230
  • Mass (Da)
    25,894
  • Last updated
    2014-04-16 v1
  • Checksum
    7A159FED949BE001
MSVRILVVDDDRAVRESLRRSLSFNGYSVELAHDGVEALDMIASDRPDALVLDVMMPRLDGLEVCRQLRGTGDDLPILVLTARDSVSERVAGLDAGADDYLPKPFALEELLARMRALLRRTKPEDAAESMAMRFSDLTLDPVTREVNRGQRRISLTRTEFALLEMLIANPRRVLTRSRILEEVWGFDFPTSGNALEVYVGYLRRKTEADGEPRLIHTVRGVGYVLRETPP

Sequence caution

The sequence CCP43731.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP43731.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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