P9WGI6 · GLYA2_MYCTO
- ProteinSerine hydroxymethyltransferase 2
- GeneglyA2
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids425 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism.
Catalytic activity
- (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + glycine + H2O = (6S)-5,6,7,8-tetrahydrofolate + L-serine
Cofactor
Pathway
One-carbon metabolism; tetrahydrofolate interconversion.
Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.
Features
Showing features for binding site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 121 | (6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Binding site | 125-127 | (6S)-5,6,7,8-tetrahydrofolate (UniProtKB | ChEBI) | |||
Site | 229 | Plays an important role in substrate specificity | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Molecular Function | cobalt ion binding | |
Molecular Function | glycine hydroxymethyltransferase activity | |
Molecular Function | pyridoxal phosphate binding | |
Molecular Function | serine binding | |
Molecular Function | zinc ion binding | |
Biological Process | folic acid metabolic process | |
Biological Process | glycine biosynthetic process from serine | |
Biological Process | L-serine catabolic process | |
Biological Process | tetrahydrofolate interconversion |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSerine hydroxymethyltransferase 2
- EC number
- Short namesSHM2; SHMT 2 ; Serine methylase 2
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionP9WGI6
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000428355 | 1-425 | Serine hydroxymethyltransferase 2 | ||
Modified residue | 230 | N6-(pyridoxal phosphate)lysine | |||
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length425
- Mass (Da)45,526
- Last updated2014-04-16 v1
- MD5 Checksum774F3FCA0EDFDA969DA405CD0722B263
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE000516 EMBL· GenBank· DDBJ | AAK44300.1 EMBL· GenBank· DDBJ | Genomic DNA |