P9WGI3 · SIGF_MYCTU

Function

function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Held in an inactive form by a cognate anti-sigma factor RsbW (UsfX) until released. Increased expression decreases growth rate, and after 3 days increases the expression of 51 loci encoding 33 protein-coding genes as well as some non-coding RNA (PubMed:22307756).

Features

Showing features for dna binding.

126120406080100120140160180200220240260
TypeIDPosition(s)Description
DNA binding231-250H-T-H motif

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Molecular FunctionDNA binding
Molecular FunctionRNA polymerase core enzyme binding
Molecular Functionsigma factor activity
Biological Processcellular response to cold
Biological Processcellular response to nitrogen starvation
Biological ProcessDNA-templated transcription initiation
Biological Processlipid metabolic process
Biological Processpositive regulation of DNA-templated transcription
Biological Processregulation of DNA-templated transcription
Biological Processresponse to anoxia
Biological Processresponse to antibiotic
Biological Processresponse to cold
Biological Processresponse to oxidative stress

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    RNA polymerase sigma factor SigF
  • Short names
    Sigma factor SigF
  • Alternative names
    • Alternative RNA polymerase sigma factor SigF
    • RNA polymerase sigma-F factor (Sigma-F factor)
    • Stress response/stationary phase sigma factor SigF

Gene names

    • Name
      sigF
    • Ordered locus names
      Rv3286c

Organism names

Accessions

  • Primary accession
    P9WGI3
  • Secondary accessions
    • F2GKV9
    • Q50547
    • Q798K1
    • Q7D5S2

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Infected guinea pigs mutant and wild-type bacteria grow comparably (PubMed:16735723).
A double rsbW-sigF disruption shows no effect in the presence or absence of rifampicin (PubMed:20729364).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004229541-261RNA polymerase sigma factor SigF

Proteomic databases

Expression

Induction

Constitutively expressed at a low level under all conditions tested, including stationary phase and several stresses. 3-fold induced during starvation (PubMed:11929527).
Positively controls expression of its own operon (rsbW-sigF)

Interaction

Subunit

Monomer. Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription. Interacts (via sigma-70 factor domain 4) with anti-sigma-F factor RsbW (UsfX) with a possible 2:1 RbsW:SigF stoichiometry, which inhibits SigF activity.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, motif.

TypeIDPosition(s)Description
Region43-112Sigma-70 factor domain-2
Motif67-70Interaction with polymerase core subunit RpoC
Region123-189Sigma-70 factor domain-3
Region209-258Sigma-70 factor domain-4

Domain

The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA. Interactions between sigma-70 factor domain-4 and anti-sigma factors prevents interaction of sigma factors with the RNA polymerase catalytic core (By similarity).

Sequence similarities

Belongs to the sigma-70 factor family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    261
  • Mass (Da)
    28,794
  • Last updated
    2014-04-16 v1
  • Checksum
    7FD4AA6AB64061AB
MTARAAGGSASRANEYADVPEMFRELVGLPAGSPEFQRHRDKIVQRCLPLADHIARRFEGRGEPRDDLIQVARVGLVNAAVRFDVKTGSDFVSFAVPTIMGEVRRHFRDNSWSVKVPRRLKELHLRLGTATADLSQRLGRAPSASELAAELGMDRAEVIEGLLAGSSYHTLSIDSGGGSDDDARAITDTLGDVDAGLDQIENREVLRPLLEALPERERTVLVLRFFDSMTQTQIAERVGISQMHVSRLLAKSLARLRDQLE

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict248in Ref. 1; AAC44103

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U41061
EMBL· GenBank· DDBJ
AAC44103.1
EMBL· GenBank· DDBJ
Genomic DNA
AL123456
EMBL· GenBank· DDBJ
CCP46105.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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