P9WGH1 · RPSC_MYCTU

Function

function

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are usually held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis; this sigma factor does not seem to have a cognate anti-sigma factor however. It has been suggested that the sigma domains 2 and 4 may interact via polar residues in this protein to autoregulate. Positively regulates expression of a small regulon of genes.

Miscellaneous

Was identified as a high-confidence drug target.

Caution

The protein has been shown to be 126 residues longer (PubMed:17145760), but in (PubMed:19118359) no evidence of this longer protein was seen.

Features

Showing features for dna binding.

118520406080100120140160180
TypeIDPosition(s)Description
DNA binding149-168H-T-H motif

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular Functionsigma factor activity
Biological ProcessDNA-templated transcription initiation
Biological Processregulation of DNA-templated transcription
Biological Processresponse to heat
Biological Processresponse to hypoxia
Biological Processresponse to water
Biological Processresponse to xenobiotic stimulus

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    ECF RNA polymerase sigma factor SigC
  • Short names
    ECF sigma factor SigC
  • Alternative names
    • Alternative RNA polymerase sigma factor SigC
    • RNA polymerase sigma-C factor (Sigma-C factor)

Gene names

    • Name
      sigC
    • ORF names
      MTCY49.08
    • Ordered locus names
      Rv2069

Organism names

Accessions

  • Primary accession
    P9WGH1
  • Secondary accessions
    • L0T8P5
    • P66809
    • Q10679

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

No effect on growth in culture, or in isolated macrophages; infected guinea pigs have a highly attenuated infection with few granulomas and no necrotic centers.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis38Blue shift in fluorescence emission, possible decrease in -10 promoter recognition.
Mutagenesis77Red shift in fluorescence emission.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000940211-185ECF RNA polymerase sigma factor SigC

Proteomic databases

Expression

Induction

The most abundant of the sigma factor transcripts, it is expressed in exponential phase; repressed by detergent (7-fold), heat shock (9-fold, 45 degrees Celsius) and in stationary phase. Not autoregulated (PubMed:22015173).

Interaction

Subunit

Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, motif.

TypeIDPosition(s)Description
Region30-94Sigma-70 factor domain-2
Motif52-55Polymerase core binding
Region123-174Sigma-70 factor domain-4

Domain

The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA (By similarity).

Sequence similarities

Belongs to the sigma-70 factor family. ECF subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    185
  • Mass (Da)
    19,966
  • Last updated
    2014-04-16 v1
  • Checksum
    FF839B4279929917
MTATASDDEAVTALALSAAKGNGRALEAFIKATQQDVWRFVAYLSDVGSADDLTQETFLRAIGAIPRFSARSSARTWLLAIARHVVADHIRHVRSRPRTTRGARPEHLIDGDRHARGFEDLVEVTTMIADLTTDQREALLLTQLLGLSYADAAAVCGCPVGTIRSRVARARDALLADAEPDDLTG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP44843.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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