P9WGH1 · RPSC_MYCTU
- ProteinECF RNA polymerase sigma factor SigC
- GenesigC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids185 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Extracytoplasmic function (ECF) sigma factors are usually held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis; this sigma factor does not seem to have a cognate anti-sigma factor however. It has been suggested that the sigma domains 2 and 4 may interact via polar residues in this protein to autoregulate. Positively regulates expression of a small regulon of genes.
Miscellaneous
Was identified as a high-confidence drug target.
Features
Showing features for dna binding.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
DNA binding | 149-168 | H-T-H motif | ||||
Sequence: YADAAAVCGCPVGTIRSRVA |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | DNA binding | |
Molecular Function | sigma factor activity | |
Biological Process | DNA-templated transcription initiation | |
Biological Process | regulation of DNA-templated transcription | |
Biological Process | response to heat | |
Biological Process | response to hypoxia | |
Biological Process | response to water | |
Biological Process | response to xenobiotic stimulus |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended nameECF RNA polymerase sigma factor SigC
- Short namesECF sigma factor SigC
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionP9WGH1
- Secondary accessions
Proteomes
Organism-specific databases
Phenotypes & Variants
Disruption phenotype
No effect on growth in culture, or in isolated macrophages; infected guinea pigs have a highly attenuated infection with few granulomas and no necrotic centers.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 38 | Blue shift in fluorescence emission, possible decrease in -10 promoter recognition. | ||||
Sequence: W → A | ||||||
Mutagenesis | 77 | Red shift in fluorescence emission. | ||||
Sequence: W → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000094021 | 1-185 | ECF RNA polymerase sigma factor SigC | |||
Sequence: MTATASDDEAVTALALSAAKGNGRALEAFIKATQQDVWRFVAYLSDVGSADDLTQETFLRAIGAIPRFSARSSARTWLLAIARHVVADHIRHVRSRPRTTRGARPEHLIDGDRHARGFEDLVEVTTMIADLTTDQREALLLTQLLGLSYADAAAVCGCPVGTIRSRVARARDALLADAEPDDLTG |
Proteomic databases
Expression
Induction
The most abundant of the sigma factor transcripts, it is expressed in exponential phase; repressed by detergent (7-fold), heat shock (9-fold, 45 degrees Celsius) and in stationary phase. Not autoregulated (PubMed:22015173).
Interaction
Subunit
Interacts transiently with the RNA polymerase catalytic core formed by RpoA, RpoB, RpoC and RpoZ (2 alpha, 1 beta, 1 beta' and 1 omega subunit) to form the RNA polymerase holoenzyme that can initiate transcription.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 30-94 | Sigma-70 factor domain-2 | ||||
Sequence: IKATQQDVWRFVAYLSDVGSADDLTQETFLRAIGAIPRFSARSSARTWLLAIARHVVADHIRHVR | ||||||
Motif | 52-55 | Polymerase core binding | ||||
Sequence: DLTQ | ||||||
Region | 123-174 | Sigma-70 factor domain-4 | ||||
Sequence: EVTTMIADLTTDQREALLLTQLLGLSYADAAAVCGCPVGTIRSRVARARDAL |
Domain
The sigma-70 factor domain-2 mediates sequence-specific interaction with the -10 element in promoter DNA, and plays an important role in melting the double-stranded DNA and the formation of the transcription bubble. The sigma-70 factor domain-2 mediates interaction with the RNA polymerase subunits RpoB and RpoC (By similarity).
The sigma-70 factor domain-4 contains a helix-turn-helix (H-T-H) motif that mediates interaction with the -35 element in promoter DNA. The domain also mediates interaction with the RNA polymerase subunit RpoA (By similarity).
Sequence similarities
Belongs to the sigma-70 factor family. ECF subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length185
- Mass (Da)19,966
- Last updated2014-04-16 v1
- ChecksumFF839B4279929917
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL123456 EMBL· GenBank· DDBJ | CCP44843.1 EMBL· GenBank· DDBJ | Genomic DNA |