P9WG29 · RPFB_MYCTU
- ProteinResuscitation-promoting factor RpfB
- GenerpfB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids362 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity.
Reduces lag phase and enhances the growth of quiescent (1 month-old culture) M.tuberculosis; works best between 8 and 128 pM. Increases the number of bacteria that can be recovered from a 3 month-old culture. Stimulates growth of stationary phase M.bovis (a slowly-growing Mycobacterium) as well as M.smegmatis cells (a fast grower). Binds N,N',N''-triacetylchitotriose (tri-NAG). A fragment (residues 194-362) hydrolyzes an artificial lysozyme substrate 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside (MUF tri-NAG). By itself has little activity on cell wall, in combination with RipA is active against cell wall extracts from a number of Actinobacteria; this activity is inhibited by PBP1A (ponA1). Sequential gene disruption indicates RpfB and RpfE are higher than RpfD and RpfC in functional hierarchy.
Miscellaneous
Was identified as a high-confidence drug target.
Activity regulation
Activity on the artificial substrate MUF tri-NAG is inhibited by 2-nitrophenylthiocyanates (NPT) compounds. The synergistic effects on peptidoglycan degradation of RipA plus RpfB are inhibited by addition of PBP1A (ponA1).
Biotechnology
A promising vaccine candidate, an rpfB-encoding DNA vaccine induces elevated cellular immune responses, confers modest but significant protection against intra-tracheal tuberculosis challenge in female C57BL/6 and BALB/c mice.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | plasma membrane | |
Molecular Function | hydrolase activity | |
Biological Process | dormancy exit of symbiont in host | |
Biological Process | negative regulation of gene expression | |
Biological Process | positive regulation of gene expression | |
Biological Process | quorum sensing | |
Biological Process | regulation of cell population proliferation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameResuscitation-promoting factor RpfB
- EC number
Gene names
Organism names
- Strains
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionP9WG29
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Lipid-anchor
Note: Localizes to the septa upon expression in M.smegmatis.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Not essential, disruption of rpfB alone has no effect on growth or survival in liquid culture, nor in mouse infection models, colonies plated over a 52-week culture period are visibly drier and more friable. Alterations in gene expression are seen. All 5 genes in this family can be deleted without affecting growth in culture, however triple deletion mutants (rpfA-rpfC-rpfB or rpfA-rpfC-rpfD) are not able to resuscitate spontaneously in the presence or absence of O2, and are attenuated in a mouse infection model.
PTM/Processing
Features
Showing features for signal, lipidation, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-23 | |||||
Sequence: MLRLVVGALLLVLAFAGGYAVAA | ||||||
Lipidation | 24 | N-palmitoyl cysteine | ||||
Sequence: C | ||||||
Lipidation | 24 | S-diacylglycerol cysteine | ||||
Sequence: C | ||||||
Chain | PRO_0000421026 | 24-362 | Resuscitation-promoting factor RpfB | |||
Sequence: CKTVTLTVDGTAMRVTTMKSRVIDIVEENGFSVDDRDDLYPAAGVQVHDADTIVLRRSRPLQISLDGHDAKQVWTTASTVDEALAQLAMTDTAPAAASRASRVPLSGMALPVVSAKTVQLNDGGLVRTVHLPAPNVAGLLSAAGVPLLQSDHVVPAATAPIVEGMQIQVTRNRIKKVTERLPLPPNARRVEDPEMNMSREVVEDPGVPGTQDVTFAVAEVNGVETGRLPVANVVVTPAHEAVVRVGTKPGTEVPPVIDGSIWDAIAGCEAGGNWAINTGNGYYGGVQFDQGTWEANGGLRYAPRADLATREEQIAVAEVTRLRQGWGAWPVCAARAGAR | ||||||
Disulfide bond | 291↔355 | |||||
Sequence: CEAGGNWAINTGNGYYGGVQFDQGTWEANGGLRYAPRADLATREEQIAVAEVTRLRQGWGAWPVC |
Keywords
- PTM
Proteomic databases
Expression
Induction
Expressed in actively growing cells.
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 192-272 | G5 | ||||
Sequence: VTRNRIKKVTERLPLPPNARRVEDPEMNMSREVVEDPGVPGTQDVTFAVAEVNGVETGRLPVANVVVTPAHEAVVRVGTKP |
Domain
An expressed fragment (residues 194-362) hydrolyzes an artificial lysozyme substrate 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside (MUF tri-NAG).
Sequence similarities
Belongs to the transglycosylase family. Rpf subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length362
- Mass (Da)38,078
- Last updated2014-04-16 v1
- Checksum02B55D8C70373D10
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL123456 EMBL· GenBank· DDBJ | CCP43759.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
KY702944 EMBL· GenBank· DDBJ | AVY54108.1 EMBL· GenBank· DDBJ | Genomic DNA |