P9WG29 · RPFB_MYCTU

Function

function

Factor that stimulates resuscitation of dormant cells. Has peptidoglycan (PG) hydrolytic activity. Active in the pM concentration range. Has little to no effect on actively-growing cells. PG fragments could either directly activate the resuscitation pathway of dormant bacteria or serve as a substrate for endogenous Rpf, resulting in low molecular weight products with resuscitation activity.
Reduces lag phase and enhances the growth of quiescent (1 month-old culture) M.tuberculosis; works best between 8 and 128 pM. Increases the number of bacteria that can be recovered from a 3 month-old culture. Stimulates growth of stationary phase M.bovis (a slowly-growing Mycobacterium) as well as M.smegmatis cells (a fast grower). Binds N,N',N''-triacetylchitotriose (tri-NAG). A fragment (residues 194-362) hydrolyzes an artificial lysozyme substrate 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside (MUF tri-NAG). By itself has little activity on cell wall, in combination with RipA is active against cell wall extracts from a number of Actinobacteria; this activity is inhibited by PBP1A (ponA1). Sequential gene disruption indicates RpfB and RpfE are higher than RpfD and RpfC in functional hierarchy.

Miscellaneous

Was identified as a high-confidence drug target.

Activity regulation

Activity on the artificial substrate MUF tri-NAG is inhibited by 2-nitrophenylthiocyanates (NPT) compounds. The synergistic effects on peptidoglycan degradation of RipA plus RpfB are inhibited by addition of PBP1A (ponA1).

Biotechnology

A promising vaccine candidate, an rpfB-encoding DNA vaccine induces elevated cellular immune responses, confers modest but significant protection against intra-tracheal tuberculosis challenge in female C57BL/6 and BALB/c mice.

GO annotations

AspectTerm
Cellular Componentextracellular region
Cellular Componentplasma membrane
Molecular Functionhydrolase activity
Biological Processdormancy exit of symbiont in host
Biological Processnegative regulation of gene expression
Biological Processpositive regulation of gene expression
Biological Processquorum sensing
Biological Processregulation of cell population proliferation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Resuscitation-promoting factor RpfB
  • EC number

Gene names

    • Name
      rpfB
    • ORF names
      MTC1237.26
    • Ordered locus names
      Rv1009

Organism names

Accessions

  • Primary accession
    P9WG29
  • Secondary accessions
    • A0A2Z3DFM7
    • F2GHD1
    • L0T719
    • O05594
    • Q7D900

Proteomes

Organism-specific databases

Subcellular Location

Cell membrane
; Lipid-anchor
Note: Localizes to the septa upon expression in M.smegmatis.

Keywords

Phenotypes & Variants

Disruption phenotype

Not essential, disruption of rpfB alone has no effect on growth or survival in liquid culture, nor in mouse infection models, colonies plated over a 52-week culture period are visibly drier and more friable. Alterations in gene expression are seen. All 5 genes in this family can be deleted without affecting growth in culture, however triple deletion mutants (rpfA-rpfC-rpfB or rpfA-rpfC-rpfD) are not able to resuscitate spontaneously in the presence or absence of O2, and are attenuated in a mouse infection model.

PTM/Processing

Features

Showing features for signal, lipidation, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-23
Lipidation24N-palmitoyl cysteine
Lipidation24S-diacylglycerol cysteine
ChainPRO_000042102624-362Resuscitation-promoting factor RpfB
Disulfide bond291↔355

Keywords

Proteomic databases

Expression

Induction

Expressed in actively growing cells.

Interaction

Subunit

Monomer (Probable). Interacts with RipA.

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain192-272G5

Domain

An expressed fragment (residues 194-362) hydrolyzes an artificial lysozyme substrate 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside (MUF tri-NAG).

Sequence similarities

Belongs to the transglycosylase family. Rpf subfamily.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    362
  • Mass (Da)
    38,078
  • Last updated
    2014-04-16 v1
  • Checksum
    02B55D8C70373D10
MLRLVVGALLLVLAFAGGYAVAACKTVTLTVDGTAMRVTTMKSRVIDIVEENGFSVDDRDDLYPAAGVQVHDADTIVLRRSRPLQISLDGHDAKQVWTTASTVDEALAQLAMTDTAPAAASRASRVPLSGMALPVVSAKTVQLNDGGLVRTVHLPAPNVAGLLSAAGVPLLQSDHVVPAATAPIVEGMQIQVTRNRIKKVTERLPLPPNARRVEDPEMNMSREVVEDPGVPGTQDVTFAVAEVNGVETGRLPVANVVVTPAHEAVVRVGTKPGTEVPPVIDGSIWDAIAGCEAGGNWAINTGNGYYGGVQFDQGTWEANGGLRYAPRADLATREEQIAVAEVTRLRQGWGAWPVCAARAGAR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL123456
EMBL· GenBank· DDBJ
CCP43759.1
EMBL· GenBank· DDBJ
Genomic DNA
KY702944
EMBL· GenBank· DDBJ
AVY54108.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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