P9WFX1 · MBTI_MYCTU
- ProteinSalicylate synthase
- GenembtI
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids450 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in the incorporation of salicylate into the virulence-conferring salicylate-based siderophore mycobactin. Catalyzes the initial conversion of chorismate to yield the intermediate isochorismate (isochorismate synthase activity), and the subsequent elimination of the enolpyruvyl side chain to give salicylate (isochorismate pyruvate-lyase activity). In the absence of magnesium, MbtI displays a chorismate mutase activity and converts chorismate to prephenate.
Catalytic activity
- chorismate = isochorismate
Cofactor
Activity regulation
Inhibited by (E)-3-(1-carboxyprop-1-enyloxy)-2-hydroxybenzoic acid (AMT), 3-(1-carboxy-2-phenylvinyloxy)-2-hydroxybenzoic acid (phenyl-AMT), 3-(1-carboxy-3-methylbut-1-enyloxy)-2-hydroxybenzoic acid, 3-(1-carboxybut-1-enyloxy)-2-hydroxybenzoic acid (ethyl-AMT), 3-(1-carboxyprop-1-enyloxy)-2-hydroxybenzoic acid (methyl-AMT), 3-(1-carboxy-2-cyclopropylethenyloxy)-2-hydroxybenzoic acid (cyclopropyl-AMT) and 3-(1-carboxy-3-methylbut-1-enyloxy)-2-hydroxybenzoic acid (isopropyl-AMT).
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
2.6 μM | isochorismate | 8 | 37 | for isochorismate pyruvate lyase activity | ||
6 μM | isochorismate | 8 | 37 | for salicylate synthase activity | ||
21 μM | isochorismate | 8 | 25 | for salicylate synthase activity | ||
26 μM | chorismate | 7.5 | 37 | for chorismate mutase activity without magnesium | ||
34 μM | chorismate | 7 | 37 | for isochorismate synthase activity |
kcat is 2.6 min-1 for isochorismate pyruvate lyase activity (at pH 8 and 37 degrees Celsius). kcat is 2.8 min-1 for salicylate synthase activity (at pH 8 and 37 degrees Celsius). kcat is 3.1 min-1 for isochorismate synthase activity (at pH 7 and 37 degrees Celsius). kcat is 4.5 min-1 for chorismate mutase activity (without magnesium at pH 7.5 and 37 degrees Celsius).
pH Dependence
At pH below 7.5, MtbI produces isochorismate and at pH 8 it produces salicylate.
Pathway
Siderophore biosynthesis; mycobactin biosynthesis.
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 252 | Proton donor | ||||
Sequence: E | ||||||
Site | 268 | Could activate a water molecule for attack at the C2 of chorismate and involved in recognition/elimination of the C4 hydroxyl | ||||
Sequence: L | ||||||
Binding site | 270-271 | substrate | ||||
Sequence: GT | ||||||
Binding site | 297 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 385 | substrate | ||||
Sequence: Y | ||||||
Binding site | 405 | substrate | ||||
Sequence: R | ||||||
Binding site | 419-421 | substrate | ||||
Sequence: GAG | ||||||
Binding site | 431 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 434 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 438 | substrate | ||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | chorismate mutase activity | |
Molecular Function | isochorismate pyruvate lyase activity | |
Molecular Function | isochorismate synthase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | oxo-acid-lyase activity | |
Biological Process | catechol-containing siderophore biosynthetic process | |
Biological Process | cellular response to iron ion starvation | |
Biological Process | response to host immune response | |
Biological Process | salicylic acid biosynthetic process | |
Biological Process | tryptophan biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameSalicylate synthase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis complex
Accessions
- Primary accessionP9WFX1
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Phenotypes & Variants
Disruption phenotype
Cells lacking this gene display a reduction in salicylic acid biosynthesis and a drastic decrease in production of mycobactin compared with the wild-type strain.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 205 | Only the chorismate mutase activity is observed. | ||||
Sequence: K → A | ||||||
Mutagenesis | 252 | No activity is observed. | ||||
Sequence: E → Q | ||||||
Mutagenesis | 268 | Only the chorismate mutase activity is observed. | ||||
Sequence: L → A | ||||||
Mutagenesis | 271 | Only the chorismate mutase activity is observed. | ||||
Sequence: T → A | ||||||
Mutagenesis | 334 | Only the chorismate mutase activity is observed. | ||||
Sequence: H → M | ||||||
Mutagenesis | 405 | Only the chorismate mutase activity is observed. | ||||
Sequence: R → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000262086 | 1-450 | Salicylate synthase | |||
Sequence: MSELSVATGAVSTASSSIPMPAGVNPADLAAELAAVVTESVDEDYLLYECDGQWVLAAGVQAMVELDSDELRVIRDGVTRRQQWSGRPGAALGEAVDRLLLETDQAFGWVAFEFGVHRYGLQQRLAPHTPLARVFSPRTRIMVSEKEIRLFDAGIRHREAIDRLLATGVREVPQSRSVDVSDDPSGFRRRVAVAVDEIAAGRYHKVILSRCVEVPFAIDFPLTYRLGRRHNTPVRSFLLQLGGIRALGYSPELVTAVRADGVVITEPLAGTRALGRGPAIDRLARDDLESNSKEIVEHAISVRSSLEEITDIAEPGSAAVIDFMTVRERGSVQHLGSTIRARLDPSSDRMAALEALFPAVTASGIPKAAGVEAIFRLDECPRGLYSGAVVMLSADGGLDAALTLRAAYQVGGRTWLRAGAGIIEESEPEREFEETCEKLSTLTPYLVARQ |
Proteomic databases
Expression
Induction
Induced by iron starvation conditions and during infection of human THP-1 macrophages. Transcriptionally repressed by IdeR and iron.
Interaction
Structure
Family & Domains
Sequence similarities
Belongs to the anthranilate synthase component I family. Salicylate synthase subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length450
- Mass (Da)48,754
- Last updated2014-04-16 v1
- Checksum79D95307E025D28A
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL123456 EMBL· GenBank· DDBJ | CCP45174.1 EMBL· GenBank· DDBJ | Genomic DNA |