P9WEZ1 · PYTI_ASPTE

  • Protein
    Thioesterase pytI
  • Gene
    pytI
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    3/5

Function

function

Thioesterase; part of the gene cluster that mediates the biosynthesis of pyranterreones, a family of antioxidative compounds (PubMed:32077283).
The first step of pyranonigrins biosynthesis is performed by the hybrid PKS-NRPS synthetase pytA that condenses 4 malonyl-CoA units ato the acetyl starter unit by the modular PKS of pytA (PubMed:32077283).
The acyl chain is then connected to an L-serine through the amide bond by the modular NRPS of pytA (PubMed:32077283).
A tetramic acid is formed and released from the PKS-NRPS pytA to give pyranterreone 5 with the help of the thioesterase pytI (PubMed:32077283).
Pyranterreone 5 could be methylated by pytC to afford pyranterreone 6 (Probable). Both pyranterreones 5 and 6 are subsequently oxidized by the FAD-linked oxidoreductase pytB and the cytochrome P450 monooxygenase pytD to form the fused gamma-pyrone core, resulting in pyranterreones 7 and 11, respectively (PubMed:32077283).
The hydroxy group at C-8 of pyranterreones 7 and 11 are dehydrated by the aspartyl protease pytH to form a delta-7 double bond to give pyranterreones 3 and 1, 2 accordingly (PubMed:32077283).
The exo-methylene of pyranterreone 3 could be reduced into a pendant methyl by reductase pytE to provide pyranterreone 4, also known as cordylactam (Probable). Pyranterreone 4 can be reconverted to pyranterreone 3 through pytB-catalyzed dehydrogenation or further oxidized to pyranterreones 9 and 10 (Probable)

Pathway

Secondary metabolite biosynthesis.

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionhydrolase activity
Biological Processbiosynthetic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Thioesterase pytI
  • EC number
  • Alternative names
    • Pyranterreones biosynthesis cluster protein I

Gene names

    • Name
      pytI
    • ORF names
      ATETN484_0003083900, g7163

Organism names

  • Taxonomic identifier
  • Strains
    • TN-484
    • MEFC01
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Circumdati

Accessions

  • Primary accession
    P9WEZ1
  • Secondary accessions
    • A0A5M3YSX7

Subcellular Location

Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane14-34Helical
Transmembrane95-115Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Impairs the production of pyranterreones.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004504731-310Thioesterase pytI

Expression

Induction

Expression is positively regulated by the cluster-specific transcription factor pytR.

Structure

Family & Domains

Features

Showing features for region, compositional bias.

Type
IDPosition(s)Description
Region168-195Disordered
Compositional bias169-185Polar residues

Sequence similarities

Belongs to the AMT4 thioesterase family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    310
  • Mass (Da)
    34,383
  • Last updated
    2020-08-12 v1
  • MD5 Checksum
    443757FE79487FE809BCE3A7E3FA1FF7
MDIVAHIQGDPGSSLTPLILIHAISGLALPYFALRPLSTDSDDGDSDKSRPVYGLSSPIFESVSAFRRHGKSLPSLALEYVRIIRREIQPRGPYLLGGWSMGGMLAIEMAAIFVAQGETVKHVVMIDSLNPEVYPPFQDSQEHQVLSTIMYNAIAWRVEGLEEACMPTLSDDASTTTSSDNSRASTDHGADSEVESEADLDDFMQQLREHVHQGMRMLASYHTLHRHIYLPDTAVTLIKCTMLGNLSPLLRASRKVFAKKNLLDPHNGWRTEQFRSFRSVPFASTHDACFDAEASEELTTILRGVLKDID

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias169-185Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BKZM02000003
EMBL· GenBank· DDBJ
GES59613.1
EMBL· GenBank· DDBJ
Genomic DNA
MN699960
EMBL· GenBank· DDBJ
QIH14018.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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