P9WEW4 · PCH13_PENCH
- ProteinSubtilisin-like serine protease EN45_076310
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids398 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Serine protease that hydrolyzes casein, gelatin and human collagen type IV, but not elastin in vitro (By similarity).
Hydrolyzes OCLN of the human lung epithelial cells at 202-Gln-|-Ser-203 and Gln-211-|-Ile-212 (By similarity).
Hydrolyzes OCLN of the human lung epithelial cells at 202-Gln-|-Ser-203 and Gln-211-|-Ile-212 (By similarity).
Activity regulation
Inhibited by phenylmethanesulfonyl fluoride (PMSF) and diethyl pyrocarbonate (DEPC), but not by benzamidine.
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 157 | Charge relay system | ||||
Sequence: D | ||||||
Active site | 188 | Charge relay system | ||||
Sequence: H | ||||||
Site | 280 | Important for catalytic activity | ||||
Sequence: N | ||||||
Active site | 343 | Charge relay system | ||||
Sequence: S |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular space | |
Molecular Function | IgE binding | |
Molecular Function | serine-type endopeptidase activity | |
Biological Process | proteolysis |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSubtilisin-like serine protease EN45_076310
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium > Penicillium chrysogenum species complex
Accessions
- Primary accessionP9WEW4
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for signal, propeptide, glycosylation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-19 | |||||
Sequence: MGFLKLLSTSLATLAVVNA | ||||||
Propeptide | PRO_0000451343 | 20-115 | Removed in mature form | |||
Sequence: GKLLTANDGDEVVPSSYIVVMNDGVSTAQFETHRNWAANVHARTRSLKGGESGPGKHFDINGMKGYSASFDDRTVKDIASDPTVKYVEPDMVVNAT | ||||||
Glycosylation | 113 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Chain | PRO_0000451344 | 116-398 | Subtilisin-like serine protease EN45_076310 | |||
Sequence: ANVVQRNAPSWGLSRISSKKSGATDYVYDSTAGEGIVIYGVDTGIDIGHADFGGRAEWGTNTADNDDTDGNGHGTHTASTAAGSKFGVAKKASVVAVKVLGADGSGTNSQVIAGMDWAVKDSKSRGATGKSVMNMSLGGAYSRAMNDAAANVVRSGVFLSVAAGNEAQDASNSSPASAPNVCTIAASTNSDGSASFTNFGSVVDLYAPGKDITAAYPGGGSKTLSGTSMAAPHVAGAAAYLMALEGVTSDKACARIVELAISSISSAPSGTTSKLLYNGINAQ | ||||||
Glycosylation | 249 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 35-113 | Inhibitor I9 | ||||
Sequence: SYIVVMNDGVSTAQFETHRNWAANVHARTRSLKGGESGPGKHFDINGMKGYSASFDDRTVKDIASDPTVKYVEPDMVVN | ||||||
Region | 124-134 | IgE-binding | ||||
Sequence: PSWGLSRISSK | ||||||
Domain | 125-398 | Peptidase S8 | ||||
Sequence: SWGLSRISSKKSGATDYVYDSTAGEGIVIYGVDTGIDIGHADFGGRAEWGTNTADNDDTDGNGHGTHTASTAAGSKFGVAKKASVVAVKVLGADGSGTNSQVIAGMDWAVKDSKSRGATGKSVMNMSLGGAYSRAMNDAAANVVRSGVFLSVAAGNEAQDASNSSPASAPNVCTIAASTNSDGSASFTNFGSVVDLYAPGKDITAAYPGGGSKTLSGTSMAAPHVAGAAAYLMALEGVTSDKACARIVELAISSISSAPSGTTSKLLYNGINAQ | ||||||
Region | 163-170 | IgE-binding | ||||
Sequence: GHADFGGR | ||||||
Region | 175-195 | Disordered | ||||
Sequence: TNTADNDDTDGNGHGTHTAST | ||||||
Compositional bias | 176-195 | Polar residues | ||||
Sequence: NTADNDDTDGNGHGTHTAST | ||||||
Region | 227-245 | IgE-binding | ||||
Sequence: IAGMDWAVKDSKSRGATGK | ||||||
Region | 310-318 | IgE-binding | ||||
Sequence: SFTNFGSVV |
Sequence similarities
Belongs to the peptidase S8 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length398
- Mass (Da)40,362
- Last updated2020-12-02 v1
- Checksum8E85FE9E942080D3
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 176-195 | Polar residues | ||||
Sequence: NTADNDDTDGNGHGTHTAST |