P9WEW3 · PCH13_PENRB
- ProteinSubtilisin-like serine protease Pen ch 13
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids398 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Serine protease (PubMed:11893850, PubMed:16436150).
Hydrolyzes casein, gelatin and human collagen type IV, but not elastin in vitro (PubMed:16436150).
Hydrolyzes OCLN of the human lung epithelial cells at 202-Gln-|-Ser-203 and Gln-211-|-Ile-212 (PubMed:16436150).
Hydrolyzes casein, gelatin and human collagen type IV, but not elastin in vitro (PubMed:16436150).
Hydrolyzes OCLN of the human lung epithelial cells at 202-Gln-|-Ser-203 and Gln-211-|-Ile-212 (PubMed:16436150).
Activity regulation
Inhibited by phenylmethanesulfonyl fluoride (PMSF) and diethyl pyrocarbonate (DEPC), but not by benzamidine.
pH Dependence
Active at neutral to pH 8 with casein as substrate.
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 157 | Charge relay system | ||||
Sequence: D | ||||||
Active site | 188 | Charge relay system | ||||
Sequence: H | ||||||
Site | 280 | Important for catalytic activity | ||||
Sequence: N | ||||||
Active site | 343 | Charge relay system | ||||
Sequence: S |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | extracellular space | |
Molecular Function | IgE binding | |
Molecular Function | serine-type endopeptidase activity | |
Biological Process | proteolysis |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSubtilisin-like serine protease Pen ch 13
- EC number
- Alternative names
- Allergen namePen ch 13
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium > Penicillium chrysogenum species complex
Accessions
- Primary accessionP9WEW3
- Secondary accessions
Subcellular Location
Phenotypes & Variants
Allergenic properties
Causes an allergic reaction in human. Binds to IgE of patients suffering from bronchial asthma (PubMed:10231324, PubMed:11893850, PubMed:12602675, PubMed:15663570).
Binds to IgE in 17% of the 212 asthmatic patients of different age (3 to 94 years old). The binding frequency and intensity to IgE increases significantly with age. Binds also to IgG and IgG4 (PubMed:12602675).
Induces histamine release from basophils of asthmatic patients (PubMed:11893850).
May contribute to asthma by damaging the barrier formed by the airway epithelium by cleaving the tight junction protein OCLN and stimulating the release of mediators, such as PGE2, CXCL8 and TGFB1, in human respiratory epithelial cells (PubMed:16436150).
Binds to IgE in 17% of the 212 asthmatic patients of different age (3 to 94 years old). The binding frequency and intensity to IgE increases significantly with age. Binds also to IgG and IgG4 (PubMed:12602675).
Induces histamine release from basophils of asthmatic patients (PubMed:11893850).
May contribute to asthma by damaging the barrier formed by the airway epithelium by cleaving the tight junction protein OCLN and stimulating the release of mediators, such as PGE2, CXCL8 and TGFB1, in human respiratory epithelial cells (PubMed:16436150).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 164 | Reduced IgE-binding. Significantly reduced IgE-binding; when associated with M-167. | ||||
Sequence: H → M | ||||||
Mutagenesis | 167 | Reduced IgE-binding. Significantly reduced IgE-binding; when associated with M-164. | ||||
Sequence: F → M | ||||||
Mutagenesis | 318-321 | Reduced IgE-binding. | ||||
Sequence: VDLY → AAL |
Keywords
- Disease
PTM/Processing
Features
Showing features for signal, propeptide, glycosylation, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-19 | |||||
Sequence: MGFLKLLSTSLATLAVVNA | ||||||
Propeptide | PRO_0000446677 | 20-115 | Removed in mature form | |||
Sequence: GKLLTANDGDEVVPSSYIVVMNDGVSTAQFETHRNWAANVHARTRSLKGGESGPGKHFDINGMKGYSASFDDRTVKDIASDPTVKYVEPDMVVNAT | ||||||
Glycosylation | 113 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Chain | PRO_5004312984 | 116-398 | Subtilisin-like serine protease Pen ch 13 | |||
Sequence: ANVVQRNAPSWGLSRISSKKSGATDYVYDSTAGEGIVIYGVDTGIDIGHADFGGRAEWGTNTADNDDTDGNGHGTHTASTAAGSKFGVAKKASVVAVKVLGADGSGTNSQVIAGMDWAVKDSKSRGATGKSVMNMSLGGAYSRAMNDAAANVVRSGVFLSVAAGNEAQDASNSSPASAPNVCTIAASTNSDGSASFTNFGSVVDLYAPGKDITAAYPGGGSKTLSGTSMAAPHVAGAAAYLMALEGVTSDKACARIVELAISSISSAPSGTTSKLLYNGINAQ | ||||||
Glycosylation | 249 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 35-113 | Inhibitor I9 | ||||
Sequence: SYIVVMNDGVSTAQFETHRNWAANVHARTRSLKGGESGPGKHFDINGMKGYSASFDDRTVKDIASDPTVKYVEPDMVVN | ||||||
Region | 124-134 | IgE-binding | ||||
Sequence: PSWGLSRISSK | ||||||
Domain | 125-398 | Peptidase S8 | ||||
Sequence: SWGLSRISSKKSGATDYVYDSTAGEGIVIYGVDTGIDIGHADFGGRAEWGTNTADNDDTDGNGHGTHTASTAAGSKFGVAKKASVVAVKVLGADGSGTNSQVIAGMDWAVKDSKSRGATGKSVMNMSLGGAYSRAMNDAAANVVRSGVFLSVAAGNEAQDASNSSPASAPNVCTIAASTNSDGSASFTNFGSVVDLYAPGKDITAAYPGGGSKTLSGTSMAAPHVAGAAAYLMALEGVTSDKACARIVELAISSISSAPSGTTSKLLYNGINAQ | ||||||
Region | 163-170 | IgE-binding | ||||
Sequence: GHADFGGR | ||||||
Region | 175-195 | Disordered | ||||
Sequence: TNTADNDDTDGNGHGTHTAST | ||||||
Compositional bias | 176-195 | Polar residues | ||||
Sequence: NTADNDDTDGNGHGTHTAST | ||||||
Region | 227-245 | IgE-binding | ||||
Sequence: IAGMDWAVKDSKSRGATGK | ||||||
Region | 310-318 | IgE-binding | ||||
Sequence: SFTNFGSVV |
Sequence similarities
Belongs to the peptidase S8 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length398
- Mass (Da)40,362
- Last updated2020-12-02 v1
- Checksum8E85FE9E942080D3
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 176-195 | Polar residues | ||||
Sequence: NTADNDDTDGNGHGTHTAST |
Keywords
- Technical term