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P9WEW2 · STS1_STEHR

Function

function

Terpene cyclase that catalyzes the cyclization of farnesyl diphosphate (FPP) to delta6-protoilludene (PubMed:24166732, PubMed:26239156).
In presence of Ca2+, a significant switch from 1,11 to a dual 1,11/1,10 cyclization occurs, producing beta-elemene as the major product, with lower levels of delta6-protoilludene and (E)-beta-caryophyllene, and traces of beta-selinene and alpha-selinene (PubMed:26239156).

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Ni2+ (UniProtKB | Rhea| CHEBI:49786 )

Co2+ (UniProtKB | Rhea| CHEBI:48828 )

Note: Mg2+ and Mn2+ are more tightly associated with the active site motifs (D(D/E)XX(D/E) and NSE) and thus lead to more efficient removal of PPi (PubMed:26239156).
In the presence of Co2+ and Ni2+ the activity is retained and delta6-protoilludene is the major volatile sesquiterpene produced (PubMed:26239156).
In the presence of Ca2+, the major sesquiterpene produced switches to beta-elemene (PubMed:26239156).

Activity regulation

Ca2+ switches the cyclization mechanism of delta6-protoilludene synthase from 1,11 to 1,10 cyclization which leads to the production of beta-elemene.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
1.52 μMfarnesyl diphosphate (FPP)
33.5 μMMg2+
8.5 μMMn2+
12.9 μMCa2+
16 μMNi2+
30.3 μMCo2+

Features

Showing features for site, binding site.

Type
IDPosition(s)Description
Site104Plays a critical role in the stabilization of intermediate cation
Binding site107Mg2+ 1 (UniProtKB | ChEBI)
Binding site107Mg2+ 2 (UniProtKB | ChEBI)
Binding site199substrate
Binding site243Mg2+ 3 (UniProtKB | ChEBI)
Binding site247Mg2+ 3 (UniProtKB | ChEBI)
Binding site251Mg2+ 3 (UniProtKB | ChEBI)
Binding site349-350substrate

GO annotations

AspectTerm
Molecular Functionlyase activity
Molecular Functionmetal ion binding

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Delta(6)-protoilludene synthase STEHIDRAFT_73029
  • EC number
  • Alternative names
    • Terpene cyclase STEHIDRAFT_73029

Gene names

    • ORF names
      STEHIDRAFT_73029

Organism names

Accessions

  • Primary accession
    P9WEW2

Proteomes

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis53Does not affect cyclization in the presence of Mg2+ or Ca2+, but impairs the activity in the presence of Ni2+ or Co2+.
Mutagenesis111Does not drastically affect cyclization in the presence of Mg2+, but impairs the activity in the presence of Ca2+.
Mutagenesis116Does not drastically affect cyclization in the presence of Mg2+ or Co2+, but severely affects the activity in the presence of Ca2+ and Ni2+.
Mutagenesis117Does not drastically affect cyclization in the presence of Mg2+ or Co2+, but severely affects the activity in the presence of Ca2+ and Ni2+.
Mutagenesis182Does not drastically affect cyclization in the presence of Mg2+, but severely affects the activity in the presence of Ca2+, Ni2+ or Co2+.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004512541-369Delta6-protoilludene synthase STEHIDRAFT_73029

Structure

Family & Domains

Features

Showing features for motif.

TypeIDPosition(s)Description
Motif107-111D(D/E)XX(D/E) motif
Motif243-251NSE motif

Domain

The 2 conserved active-site motifs D(D/E)XX(D/E) and NSE are required for coordinating the divalent metal ions that stabilize the PPi moiety of the substrate.

Sequence similarities

Belongs to the terpene synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    369
  • Mass (Da)
    41,515
  • Last updated
    2020-10-07 v1
  • MD5 Checksum
    EAA59A1C0CDDD2CD901B69F4580E0482
MAVATSVATPVPTPAYSAGRAPAKEKKIYLPDTLAEWPWPRAINPHYAEAKEESQAWAASFNAFSPKAQHAFNRCDFNLLASLAYPLATKHGCRSGCDLMNLFFVIDEYSDIAPVEEVRQQKDIVMDALRNPHKPRPEGEWVGGEVARQFWALTITNASAQSQKHFIETFDEYLDSVVQQAEDRSESRIRDIQSYIDVRRNTIGAKPSFALLELDMDLPDEVLAHPTIQSLSLATIDMLCLGNDIVSYNLEQARGDASHNIITIVMNELNLDVNGAMRWVGDFHKQLEKQFFEAFNNLPKWGNAELDAQIAVYCDGLGNWVRANDQWSFESERYFGARGLEIMETKTLAMMPIQRTEALGPQLVDDSIL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
JH687380
EMBL· GenBank· DDBJ
EIM91236.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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