P9WEW2 · STS1_STEHR
- ProteinDelta(6)-protoilludene synthase STEHIDRAFT_73029
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids369 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Terpene cyclase that catalyzes the cyclization of farnesyl diphosphate (FPP) to delta6-protoilludene (PubMed:24166732, PubMed:26239156).
In presence of Ca2+, a significant switch from 1,11 to a dual 1,11/1,10 cyclization occurs, producing beta-elemene as the major product, with lower levels of delta6-protoilludene and (E)-beta-caryophyllene, and traces of beta-selinene and alpha-selinene (PubMed:26239156).
In presence of Ca2+, a significant switch from 1,11 to a dual 1,11/1,10 cyclization occurs, producing beta-elemene as the major product, with lower levels of delta6-protoilludene and (E)-beta-caryophyllene, and traces of beta-selinene and alpha-selinene (PubMed:26239156).
Catalytic activity
- (2E,6E)-farnesyl diphosphate = Delta6-protoilludene + diphosphateThis reaction proceeds in the forward direction.
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Ca2+ (UniProtKB | Rhea| CHEBI:29108 )
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )
Co2+ (UniProtKB | Rhea| CHEBI:48828 )
Note: Mg2+ and Mn2+ are more tightly associated with the active site motifs (D(D/E)XX(D/E) and NSE) and thus lead to more efficient removal of PPi (PubMed:26239156).
In the presence of Co2+ and Ni2+ the activity is retained and delta6-protoilludene is the major volatile sesquiterpene produced (PubMed:26239156).
In the presence of Ca2+, the major sesquiterpene produced switches to beta-elemene (PubMed:26239156).
In the presence of Co2+ and Ni2+ the activity is retained and delta6-protoilludene is the major volatile sesquiterpene produced (PubMed:26239156).
In the presence of Ca2+, the major sesquiterpene produced switches to beta-elemene (PubMed:26239156).
Activity regulation
Ca2+ switches the cyclization mechanism of delta6-protoilludene synthase from 1,11 to 1,10 cyclization which leads to the production of beta-elemene.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
1.52 μM | farnesyl diphosphate (FPP) | |||||
33.5 μM | Mg2+ | |||||
8.5 μM | Mn2+ | |||||
12.9 μM | Ca2+ | |||||
16 μM | Ni2+ | |||||
30.3 μM | Co2+ |
Features
Showing features for site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Site | 104 | Plays a critical role in the stabilization of intermediate cation | |||
Binding site | 107 | Mg2+ 1 (UniProtKB | ChEBI) | |||
Binding site | 107 | Mg2+ 2 (UniProtKB | ChEBI) | |||
Binding site | 199 | substrate | |||
Binding site | 243 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 247 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 251 | Mg2+ 3 (UniProtKB | ChEBI) | |||
Binding site | 349-350 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | lyase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDelta(6)-protoilludene synthase STEHIDRAFT_73029
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Basidiomycota > Agaricomycotina > Agaricomycetes > Russulales > Stereaceae > Stereum
Accessions
- Primary accessionP9WEW2
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 53 | Does not affect cyclization in the presence of Mg2+ or Ca2+, but impairs the activity in the presence of Ni2+ or Co2+. | |||
Mutagenesis | 111 | Does not drastically affect cyclization in the presence of Mg2+, but impairs the activity in the presence of Ca2+. | |||
Mutagenesis | 116 | Does not drastically affect cyclization in the presence of Mg2+ or Co2+, but severely affects the activity in the presence of Ca2+ and Ni2+. | |||
Mutagenesis | 117 | Does not drastically affect cyclization in the presence of Mg2+ or Co2+, but severely affects the activity in the presence of Ca2+ and Ni2+. | |||
Mutagenesis | 182 | Does not drastically affect cyclization in the presence of Mg2+, but severely affects the activity in the presence of Ca2+, Ni2+ or Co2+. | |||
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000451254 | 1-369 | Delta6-protoilludene synthase STEHIDRAFT_73029 | ||
Structure
Family & Domains
Features
Showing features for motif.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Motif | 107-111 | D(D/E)XX(D/E) motif | |||
Motif | 243-251 | NSE motif | |||
Domain
The 2 conserved active-site motifs D(D/E)XX(D/E) and NSE are required for coordinating the divalent metal ions that stabilize the PPi moiety of the substrate.
Sequence similarities
Belongs to the terpene synthase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length369
- Mass (Da)41,515
- Last updated2020-10-07 v1
- MD5 ChecksumEAA59A1C0CDDD2CD901B69F4580E0482
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JH687380 EMBL· GenBank· DDBJ | EIM91236.1 EMBL· GenBank· DDBJ | Genomic DNA |