P9WEV9 · MDPH2_EMENI

Function

function

Anthrone oxygenase; part of the gene cluster that mediates the biosynthesis of monodictyphenone, a prenyl xanthone derivative (PubMed:20139316, PubMed:21351751, PubMed:22730213).
The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase (PKS) mdpG (PubMed:20139316).
The atrochrysone carboxyl ACP thioesterase mdpF then breaks the thioester bond and releases the atrochrysone carboxylic acid from mdpG (PubMed:20139316).
The atrochrysone carboxylic acid is then converted to atrochrysone which is further transformed into emodin anthrone by mdpH-1 and mdpH-2 (PubMed:20139316).
Emodin is further modified to yield monodictyphenone via several steps involving mdpB, mdpC mdpJ, mdpK and mdpL (PubMed:20139316, PubMed:21351751).
These enzymes with xptA, xptB and xptC are also proposed to be involved in the synthesis of shamixanthone from emodin (PubMed:22730213).
Especially, direct reduction of emodin by the short chain dehydrogenase mdpC followed by dehydration catalyzed by the scytalone dehydratase-like protein mdpB gives loss of oxygen and formation of chrysophanol intermediate in two simple steps (PubMed:22730213).

Catalytic activity

Pathway

Secondary metabolite biosynthesis.

GO annotations

AspectTerm
Cellular Componentmembrane
Molecular Functionmonooxygenase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Anthrone oxygenase
  • EC number
  • Alternative names
    • Monodictyphenone synthesis protein H-2

Gene names

    • Name
      mdpH-2
    • ORF names
      ANIA_10022

Organism names

Accessions

  • Primary accession
    P9WEV9
  • Secondary accessions
    • C8VQ65
    • Q5BH29

Proteomes

Subcellular Location

Membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane12-32Helical
Transmembrane54-74Helical
Transmembrane86-106Helical
Transmembrane172-192Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Impairs the production of monodictyphenone, but leads to the accumulation of endocrocin (PubMed:20139316, PubMed:21351751).

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00004508711-192Anthrone oxygenase
Glycosylation130N-linked (GlcNAc...) asparagine
Glycosylation138N-linked (GlcNAc...) asparagine
Glycosylation147N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Family & Domains

Sequence similarities

Belongs to the anthrone oxygenase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    192
  • Mass (Da)
    20,918
  • Last updated
    2020-10-07 v1
  • Checksum
    2814E86A18EA220E
MASLTTLKNTAIVTGSFLSGAMITLSTITVPVLLETSTHPPQLLHQWVRTYHYGHISLPTISIATAILYFYIAAYQGAREQPWRKAALVGFLTIVMVPFTWIVMSSTNGMLFGLEAGNRDHSQFFEQGANRSGLKGANSSQSHGLGNVSVGIGVEMATLEGVRELLVRWKWMHLVRSLFPLMAAVLGVGICV

Sequence caution

The sequence CBF90095.1 differs from that shown. Reason: Erroneous gene model prediction The predicted gene ANIA_10022 has been split into 2 genes: mdpH-1 and mdpH-2.
The sequence EAA66024.1 differs from that shown. Reason: Erroneous gene model prediction The predicted gene AN0151 has been split into 2 genes: ANIA_10022 and ANIA_10035. ANIA_10022 has been further split into mdpH-1 and mdpH-2.

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BN001308
EMBL· GenBank· DDBJ
CBF90095.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.
AACD01000005
EMBL· GenBank· DDBJ
EAA66024.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.

Genome annotation databases

Similar Proteins

Disclaimer

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