P9WEP4 · OLCL_PENCN
- ProteinMFS-type transporter olcL
- GeneolcL
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids579 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
MFS-type transporter; part of the gene cluster that mediates the biosynthesis of 15-deoxyoxalicine B (PubMed:30090271).
The first step of the pathway is the synthesis of nicotinyl-CoA from nicotinic acid by the nicotinic acid-CoA ligase olcI (PubMed:30090271).
Nicotinyl-CoA is then a substrate of polyketide synthase olcA to produce 4-hydroxy-6-(3-pyridinyl)-2H-pyran-2-one (HPPO) which is further prenylated by the polyprenyl transferase olcH to yield geranylgeranyl-HPPO (PubMed:30090271).
Geranylgeranyl pyrophosphate is provided by the cluster-specific geranylgeranyl pyrophosphate synthase olcC (PubMed:30090271).
The FAD-dependent monooxygenase olcE catalyzes the epoxidation of geranylgeranyl-HPPO and the terpene cyclase olcD catalyzes the cyclization of the terpenoid component, resulting in the formation of the tricyclic terpene moiety seen in predecaturin E (PubMed:30090271).
The cytochrome P450 monooxygenase then catalyzes the allylic oxidation of predecaturin E, which is followed by spirocylization with concomitant loss of one molecule of water to form decaturin E (PubMed:30090271).
Decaturin E is the substrate of the cytochrome P450 monooxygenase olcJ which hydroxylates it at the C-29 position to form decaturin F (PubMed:30090271).
The short-chain dehydrogenase/reductase olcF may catalyze the oxidation of decaturin F to generate the 29-hydroxyl-27-one intermediate, and subsequent hemiacetal formation probably leads to the formation of decaturin C (Probable). The dioxygenase olcK may be a peroxisomal enzyme that catalyzes the hydroxylation of decaturin C into decaturin A once decaturin C is shuttled into the peroxisome by the MFS transporter olcL (Probable). Finally the cytochrome P450 monooxygenase olcB catalyzes the oxidative rearrangement to yield 15-deoxyoxalicine B (PubMed:30090271).
In the absence of olcJ, decaturin E may be shunted to a pathway in which it is oxidized to a ketone, possibly by olcF, to form decaturin D, which undergoes further allylic oxidation to yield decaturin G (PubMed:30090271).
Moreover, in the absence of oclK or oclL, oclB can convert decaturin C into 15-deoxyoxalicine A (PubMed:30090271).
The first step of the pathway is the synthesis of nicotinyl-CoA from nicotinic acid by the nicotinic acid-CoA ligase olcI (PubMed:30090271).
Nicotinyl-CoA is then a substrate of polyketide synthase olcA to produce 4-hydroxy-6-(3-pyridinyl)-2H-pyran-2-one (HPPO) which is further prenylated by the polyprenyl transferase olcH to yield geranylgeranyl-HPPO (PubMed:30090271).
Geranylgeranyl pyrophosphate is provided by the cluster-specific geranylgeranyl pyrophosphate synthase olcC (PubMed:30090271).
The FAD-dependent monooxygenase olcE catalyzes the epoxidation of geranylgeranyl-HPPO and the terpene cyclase olcD catalyzes the cyclization of the terpenoid component, resulting in the formation of the tricyclic terpene moiety seen in predecaturin E (PubMed:30090271).
The cytochrome P450 monooxygenase then catalyzes the allylic oxidation of predecaturin E, which is followed by spirocylization with concomitant loss of one molecule of water to form decaturin E (PubMed:30090271).
Decaturin E is the substrate of the cytochrome P450 monooxygenase olcJ which hydroxylates it at the C-29 position to form decaturin F (PubMed:30090271).
The short-chain dehydrogenase/reductase olcF may catalyze the oxidation of decaturin F to generate the 29-hydroxyl-27-one intermediate, and subsequent hemiacetal formation probably leads to the formation of decaturin C (Probable). The dioxygenase olcK may be a peroxisomal enzyme that catalyzes the hydroxylation of decaturin C into decaturin A once decaturin C is shuttled into the peroxisome by the MFS transporter olcL (Probable). Finally the cytochrome P450 monooxygenase olcB catalyzes the oxidative rearrangement to yield 15-deoxyoxalicine B (PubMed:30090271).
In the absence of olcJ, decaturin E may be shunted to a pathway in which it is oxidized to a ketone, possibly by olcF, to form decaturin D, which undergoes further allylic oxidation to yield decaturin G (PubMed:30090271).
Moreover, in the absence of oclK or oclL, oclB can convert decaturin C into 15-deoxyoxalicine A (PubMed:30090271).
Miscellaneous
The 15-deoxyoxalicine B cluster is a rare cluster that contains its own geranylgeranyl pyrophosphate synthase (olcC), in contrast to other related clusters which rely on a FPP/GGPP synthase localized outside of the cluster.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | peroxisomal membrane | |
Molecular Function | transmembrane transporter activity |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameMFS-type transporter olcL
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium
Accessions
- Primary accessionP9WEP4
Subcellular Location
UniProt Annotation
GO Annotation
Peroxisome membrane ; Multi-pass membrane protein
Note: OlcL may be inserted in the peroxisomal membrane viathe import receptor pex19.
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 85-105 | Helical | ||||
Sequence: LAAIMIGVCLAVFSMALDNTI | ||||||
Transmembrane | 121-141 | Helical | ||||
Sequence: GDVGWYGSVYPLTNCCLTLVF | ||||||
Transmembrane | 159-179 | Helical | ||||
Sequence: AVFEIGSLICGATPSSLGLII | ||||||
Transmembrane | 183-203 | Helical | ||||
Sequence: IAGLGSSGIYLGSMIILSQSV | ||||||
Transmembrane | 214-234 | Helical | ||||
Sequence: LVGGLYGVAGVAGPLLGGAFT | ||||||
Transmembrane | 241-261 | Helical | ||||
Sequence: WCFYINPLFGAVTALFILLFF | ||||||
Transmembrane | 282-302 | Helical | ||||
Sequence: LIGLFFFLPGMISLLLALQWG | ||||||
Transmembrane | 310-330 | Helical | ||||
Sequence: SGRIIGLFVCSICLLSIFIMV | ||||||
Transmembrane | 355-375 | Helical | ||||
Sequence: LFNFCITGSFLVFSYYLPVWF | ||||||
Transmembrane | 388-408 | Helical | ||||
Sequence: LMNLPMLLGVILCSIISGYGV | ||||||
Transmembrane | 411-431 | Helical | ||||
Sequence: IGYYTPFMYAAPIVSAIGAGL | ||||||
Transmembrane | 439-459 | Helical | ||||
Sequence: FGPSQWIGYQALYGIGLGLGL | ||||||
Transmembrane | 479-501 | Helical | ||||
Sequence: IAIVTFIQSLGGSVSVSIAQNVF | ||||||
Transmembrane | 553-573 | Helical | ||||
Sequence: FYVGAAFSVLAMIGALPIQWI |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Abolishes the production of 15-deoxyoxalicine B and accumulates decaturin C and 5-deoxyoxalicine A.
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000453896 | 1-579 | MFS-type transporter olcL | |||
Sequence: MANIGGSNAVSSAQGSQISDSPTTVDDRLDEHKETSTQSIDHSENITQSPTSLQKPPDESNATPVGFGEDGCQSDSQEYPNSWRLAAIMIGVCLAVFSMALDNTILATAIPKITDQFTSLGDVGWYGSVYPLTNCCLTLVFGKLYTFYSTKWVYLSALAVFEIGSLICGATPSSLGLIIGRAIAGLGSSGIYLGSMIILSQSVPLQKRPLFTSLVGGLYGVAGVAGPLLGGAFTDYVSWRWCFYINPLFGAVTALFILLFFDGKEPIKSPGKIKEQISQFDLIGLFFFLPGMISLLLALQWGGQQYNWQSGRIIGLFVCSICLLSIFIMVQWRQKEKATVTLRMIKNKNVWGASLFNFCITGSFLVFSYYLPVWFQSIKNVSATKSGLMNLPMLLGVILCSIISGYGVGRIGYYTPFMYAAPIVSAIGAGLLSTFQANFGPSQWIGYQALYGIGLGLGLSQPIVVIQAAIPLIDIPSAIAIVTFIQSLGGSVSVSIAQNVFRNELLRGLAQNAPKVDAHKLITAGPTTLRYVVPAELLERVLVAYNSAITHAFYVGAAFSVLAMIGALPIQWISVKGRE | ||||||
Glycosylation | 45 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 380 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
PTM databases
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-23 | Polar residues | ||||
Sequence: MANIGGSNAVSSAQGSQISDSPT | ||||||
Region | 1-75 | Disordered | ||||
Sequence: MANIGGSNAVSSAQGSQISDSPTTVDDRLDEHKETSTQSIDHSENITQSPTSLQKPPDESNATPVGFGEDGCQSD | ||||||
Compositional bias | 24-39 | Basic and acidic residues | ||||
Sequence: TVDDRLDEHKETSTQS | ||||||
Compositional bias | 40-59 | Polar residues | ||||
Sequence: IDHSENITQSPTSLQKPPDE |
Sequence similarities
Belongs to the major facilitator superfamily. TCR/Tet family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length579
- Mass (Da)62,278
- Last updated2021-09-29 v1
- Checksum8A8CAA30E87A5691
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-23 | Polar residues | ||||
Sequence: MANIGGSNAVSSAQGSQISDSPT | ||||||
Compositional bias | 24-39 | Basic and acidic residues | ||||
Sequence: TVDDRLDEHKETSTQS | ||||||
Compositional bias | 40-59 | Polar residues | ||||
Sequence: IDHSENITQSPTSLQKPPDE |