P9WEP4 · OLCL_PENCN

  • Protein
    MFS-type transporter olcL
  • Gene
    olcL
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

MFS-type transporter; part of the gene cluster that mediates the biosynthesis of 15-deoxyoxalicine B (PubMed:30090271).
The first step of the pathway is the synthesis of nicotinyl-CoA from nicotinic acid by the nicotinic acid-CoA ligase olcI (PubMed:30090271).
Nicotinyl-CoA is then a substrate of polyketide synthase olcA to produce 4-hydroxy-6-(3-pyridinyl)-2H-pyran-2-one (HPPO) which is further prenylated by the polyprenyl transferase olcH to yield geranylgeranyl-HPPO (PubMed:30090271).
Geranylgeranyl pyrophosphate is provided by the cluster-specific geranylgeranyl pyrophosphate synthase olcC (PubMed:30090271).
The FAD-dependent monooxygenase olcE catalyzes the epoxidation of geranylgeranyl-HPPO and the terpene cyclase olcD catalyzes the cyclization of the terpenoid component, resulting in the formation of the tricyclic terpene moiety seen in predecaturin E (PubMed:30090271).
The cytochrome P450 monooxygenase then catalyzes the allylic oxidation of predecaturin E, which is followed by spirocylization with concomitant loss of one molecule of water to form decaturin E (PubMed:30090271).
Decaturin E is the substrate of the cytochrome P450 monooxygenase olcJ which hydroxylates it at the C-29 position to form decaturin F (PubMed:30090271).
The short-chain dehydrogenase/reductase olcF may catalyze the oxidation of decaturin F to generate the 29-hydroxyl-27-one intermediate, and subsequent hemiacetal formation probably leads to the formation of decaturin C (Probable). The dioxygenase olcK may be a peroxisomal enzyme that catalyzes the hydroxylation of decaturin C into decaturin A once decaturin C is shuttled into the peroxisome by the MFS transporter olcL (Probable). Finally the cytochrome P450 monooxygenase olcB catalyzes the oxidative rearrangement to yield 15-deoxyoxalicine B (PubMed:30090271).
In the absence of olcJ, decaturin E may be shunted to a pathway in which it is oxidized to a ketone, possibly by olcF, to form decaturin D, which undergoes further allylic oxidation to yield decaturin G (PubMed:30090271).
Moreover, in the absence of oclK or oclL, oclB can convert decaturin C into 15-deoxyoxalicine A (PubMed:30090271).

Miscellaneous

The 15-deoxyoxalicine B cluster is a rare cluster that contains its own geranylgeranyl pyrophosphate synthase (olcC), in contrast to other related clusters which rely on a FPP/GGPP synthase localized outside of the cluster.

GO annotations

AspectTerm
Cellular Componentperoxisomal membrane
Molecular Functiontransmembrane transporter activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    MFS-type transporter olcL
  • Alternative names
    • 15-deoxyoxalicine B biosynthesis cluster protein L

Gene names

    • Name
      olcL

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Penicillium

Accessions

  • Primary accession
    P9WEP4

Subcellular Location

Peroxisome membrane
; Multi-pass membrane protein
Note: OlcL may be inserted in the peroxisomal membrane viathe import receptor pex19.

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane85-105Helical
Transmembrane121-141Helical
Transmembrane159-179Helical
Transmembrane183-203Helical
Transmembrane214-234Helical
Transmembrane241-261Helical
Transmembrane282-302Helical
Transmembrane310-330Helical
Transmembrane355-375Helical
Transmembrane388-408Helical
Transmembrane411-431Helical
Transmembrane439-459Helical
Transmembrane479-501Helical
Transmembrane553-573Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Abolishes the production of 15-deoxyoxalicine B and accumulates decaturin C and 5-deoxyoxalicine A.

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00004538961-579MFS-type transporter olcL
Glycosylation45N-linked (GlcNAc...) asparagine
Glycosylation380N-linked (GlcNAc...) asparagine

Keywords

PTM databases

Structure

Family & Domains

Features

Showing features for compositional bias, region.

TypeIDPosition(s)Description
Compositional bias1-23Polar residues
Region1-75Disordered
Compositional bias24-39Basic and acidic residues
Compositional bias40-59Polar residues

Sequence similarities

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    579
  • Mass (Da)
    62,278
  • Last updated
    2021-09-29 v1
  • Checksum
    8A8CAA30E87A5691
MANIGGSNAVSSAQGSQISDSPTTVDDRLDEHKETSTQSIDHSENITQSPTSLQKPPDESNATPVGFGEDGCQSDSQEYPNSWRLAAIMIGVCLAVFSMALDNTILATAIPKITDQFTSLGDVGWYGSVYPLTNCCLTLVFGKLYTFYSTKWVYLSALAVFEIGSLICGATPSSLGLIIGRAIAGLGSSGIYLGSMIILSQSVPLQKRPLFTSLVGGLYGVAGVAGPLLGGAFTDYVSWRWCFYINPLFGAVTALFILLFFDGKEPIKSPGKIKEQISQFDLIGLFFFLPGMISLLLALQWGGQQYNWQSGRIIGLFVCSICLLSIFIMVQWRQKEKATVTLRMIKNKNVWGASLFNFCITGSFLVFSYYLPVWFQSIKNVSATKSGLMNLPMLLGVILCSIISGYGVGRIGYYTPFMYAAPIVSAIGAGLLSTFQANFGPSQWIGYQALYGIGLGLGLSQPIVVIQAAIPLIDIPSAIAIVTFIQSLGGSVSVSIAQNVFRNELLRGLAQNAPKVDAHKLITAGPTTLRYVVPAELLERVLVAYNSAITHAFYVGAAFSVLAMIGALPIQWISVKGRE

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias1-23Polar residues
Compositional bias24-39Basic and acidic residues
Compositional bias40-59Polar residues

Similar Proteins

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