P9WEM0 · ASTK_ASPOR
- ProteinSesquiterpene phosphatase astK
- GeneastK
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids196 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score2/5
Function
function
Sesquiterpene phosphatase; part of the gene cluster that mediates the biosynthesis of astellolides, drimane-type sesquiterpene esters that show antimicrobial, anti-inflammatory, and anti-tumor activities (PubMed:27628599).
The first step in astellolide biosynthesis is performed by the sesquiterpene cyclase astC that catalyzes the formation of drimanyl pyrophosphate from farnesyl pyrophosphate (PubMed:27628599).
Drimanyl pyrophosphate is then dephosphorylated by the sesquiterpene phosphatase astI to produce drimanyl monophosphate which is further dephosphorylated to drim-8-ene-11-ol by atsK (PubMed:27628599).
Drim-8-ene-11-ol is converted to confertifolin, probably by the cytochrome P450 monooxygenase astD and/or the dehydrogenase astE (PubMed:27628599).
The cytochrome P450 monooxygenases astB, astF and astJ then hydroxylate confertifolin at C6, C14, or C15 to form trihydroxy confertifolin (PubMed:27628599).
The nonribosomal peptide synthetase astA catalyzes ester bond formation between trihydroxy contifolin and benzoic acid (BA) or 4-hydroxy benzoic acid (4HBA), leading to the formation of dideacetyl astellolides A and B, respectively (PubMed:27628599).
Finally, the O-acetyltransferase astG converts dideacetyl astellolides A and B into deacetyl astellolides A and B (PubMed:27628599).
The first step in astellolide biosynthesis is performed by the sesquiterpene cyclase astC that catalyzes the formation of drimanyl pyrophosphate from farnesyl pyrophosphate (PubMed:27628599).
Drimanyl pyrophosphate is then dephosphorylated by the sesquiterpene phosphatase astI to produce drimanyl monophosphate which is further dephosphorylated to drim-8-ene-11-ol by atsK (PubMed:27628599).
Drim-8-ene-11-ol is converted to confertifolin, probably by the cytochrome P450 monooxygenase astD and/or the dehydrogenase astE (PubMed:27628599).
The cytochrome P450 monooxygenases astB, astF and astJ then hydroxylate confertifolin at C6, C14, or C15 to form trihydroxy confertifolin (PubMed:27628599).
The nonribosomal peptide synthetase astA catalyzes ester bond formation between trihydroxy contifolin and benzoic acid (BA) or 4-hydroxy benzoic acid (4HBA), leading to the formation of dideacetyl astellolides A and B, respectively (PubMed:27628599).
Finally, the O-acetyltransferase astG converts dideacetyl astellolides A and B into deacetyl astellolides A and B (PubMed:27628599).
Catalytic activity
- (S,S)-drim-8-en-11-yl phosphate + H2O = (S,S)-drim-8-en-11-ol + phosphateThis reaction proceeds in the forward direction.
Pathway
Secondary metabolite biosynthesis; terpenoid biosynthesis.
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSesquiterpene phosphatase astK
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Aspergillus > Aspergillus subgen. Circumdati
Accessions
- Primary accessionP9WEM0
Proteomes
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000461392 | 1-196 | Sesquiterpene phosphatase astK | ||
Expression
Induction
Expression is regulated by the secondary metabolite regulator cclA.
Structure
Family & Domains
Sequence
- Sequence statusComplete
- Length196
- Mass (Da)21,913
- Last updated2024-11-27 v1
- Checksum1E04E33E04A5BCEB
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BA000051 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |