P98183 · C71DC_CATRO

Function

function

Involved in the flower biosynthesis of vindoline, a precursor of vinblastine and vincristine (PubMed:10481044, PubMed:12228585, PubMed:24108213).
Hydroxylates specifically tabersonine, 2,3-dihydrotabersonine and 2,3-dihydro-3-hydroxytabersonine, but has no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline and catharanthine (PubMed:24108213).

Miscellaneous

Tabersonine 16-hydroxylation is orchestrated in an organ-dependent manner by two genes including CYP71D351, which encodes the T16H2 isoform acting in the foliar vindoline biosynthesis, and CYP71D12, which encodes the T16H1 isoform acting in the flower vindoline biosynthesis.

Catalytic activity

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
11 μMtabersonine in a T16H/16OMT coupled system
350 nMtabersonine
14 μMNADPH in a T16H/16OMT coupled system
concentrations of tabersonine greater than 30 uM are inhibitory.

pH Dependence

Optimum pH is 7.5 in phosphate buffer and 8.0 in TRIS-HCl.

Temperature Dependence

Stable at 30 degrees Celsius.

Pathway

Alkaloid biosynthesis; vindoline biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site447Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functiontabersonine 16-hydroxylase activity
Biological Processindole alkaloid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Tabersonine 16-hydroxylase 1
  • EC number
  • Alternative names
    • Cytochrome P450 71D12

Gene names

    • Name
      CYP71D12
    • Synonyms
      T16H1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Gentianales > Apocynaceae > Rauvolfioideae > Vinceae > Catharanthinae > Catharanthus

Accessions

  • Primary accession
    P98183
  • Secondary accessions
    • C0KYN4

Subcellular Location

Endoplasmic reticulum membrane
; Single-pass membrane protein
Note: The ER localization is dependent on the presence of the putative transmembrane helix.

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane1-21Helical

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003107281-506Tabersonine 16-hydroxylase 1

Expression

Tissue specificity

Predominantly expressed in young leaves of mature plants (PubMed:12228585, PubMed:16262708).
Low expression in roots and flowers, but not detected in stems and old leaves (PubMed:12228585, PubMed:16262708).
Found predominantly in leaf epidermis (PubMed:12228585, PubMed:16262708).
Barely detected in roots, internodes, young and mature leaves, and flower buds, but relatively abundant in fully developed flowers (PubMed:24108213).
Not detected in leaf epidermal cells (PubMed:24108213).

Induction

Up-regulated by methyl jasmonate (PubMed:24108213).
Inhibited by cytochrome c, CO, clotrimazole, miconazole, tricliphane, flusilazole and tetcyclasis, and with a lower efficiency, by 1-phenylimidazole and piperonylbutoxide. Not inhibited by potassium cyanide and sodium azide

Developmental stage

Peak of expression after light treatment at 9 days of seedling development.

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    506
  • Mass (Da)
    58,079
  • Last updated
    2017-05-10 v2
  • Checksum
    485FF14E6D34901B
MEFYYFLYLAFLLFCFILSKTTKKFGQNSQYSNHDELPPGPPQIPILGNAHQLSGGHTHHILRDLAKKYGPLMHLKIGEVSTIVASSPQIAEEIFRTHDILFADRPSNLESFKIVSYDFSDMVVSPYGNYWRQLRKISMMELLSQKSVQSFRSIREEEVLNFIKSIGSKEGTRINLSKEISLLIYGITTRAAFGEKNKNTEEFIRLLDQLTKAVAEPNIADMFPSLKFLQLISTSKYKIEKIHKQFDVIVETILKGHKEKINKPLSQENGEKKEDLVDVLLNIQRRNDFEAPLGDKNIKAIIFNIFSAGTETSSTTVDWAMCEMIKNPTVMKKAQEEVRKVFNEEGNVDETKLHQLKYLQAVIKETLRLHPPVPLLLPRECREQCKIKGYTIPSKSRVIVNAWAIGRDPNYWIEPEKFNPDRFLESKVDFKGNSFEYLPFGGGRRICPGITFALANIELPLAQLLFHFDWQSNTEKLNMKESRGVTVRREDDLYLTPVNFSSSSPA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
FJ647194
EMBL· GenBank· DDBJ
ACM92061.1
EMBL· GenBank· DDBJ
mRNA
AJ238612
EMBL· GenBank· DDBJ
CAB56503.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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