P98183 · C71DC_CATRO
- ProteinTabersonine 16-hydroxylase 1
- GeneCYP71D12
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids506 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Involved in the flower biosynthesis of vindoline, a precursor of vinblastine and vincristine (PubMed:10481044, PubMed:12228585, PubMed:24108213).
Hydroxylates specifically tabersonine, 2,3-dihydrotabersonine and 2,3-dihydro-3-hydroxytabersonine, but has no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline and catharanthine (PubMed:24108213).
Hydroxylates specifically tabersonine, 2,3-dihydrotabersonine and 2,3-dihydro-3-hydroxytabersonine, but has no activity with naringenin, tryptamine, secologanin, strictosidine, ajmalicine, vindoline and catharanthine (PubMed:24108213).
Miscellaneous
Tabersonine 16-hydroxylation is orchestrated in an organ-dependent manner by two genes including CYP71D351, which encodes the T16H2 isoform acting in the foliar vindoline biosynthesis, and CYP71D12, which encodes the T16H1 isoform acting in the flower vindoline biosynthesis.
Catalytic activity
- --tabersonine + reduced [NADPH--hemoprotein reductase] + O2 = 16-hydroxytabersonine + oxidized [NADPH--hemoprotein reductase] + H2O + H+
Cofactor
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
11 μM | tabersonine in a T16H/16OMT coupled system | |||||
350 nM | tabersonine | |||||
14 μM | NADPH in a T16H/16OMT coupled system |
concentrations of tabersonine greater than 30 uM are inhibitory.
pH Dependence
Optimum pH is 7.5 in phosphate buffer and 8.0 in TRIS-HCl.
Temperature Dependence
Stable at 30 degrees Celsius.
Pathway
Alkaloid biosynthesis; vindoline biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 447 | Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | tabersonine 16-hydroxylase activity | |
Biological Process | indole alkaloid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTabersonine 16-hydroxylase 1
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Gentianales > Apocynaceae > Rauvolfioideae > Vinceae > Catharanthinae > Catharanthus
Accessions
- Primary accessionP98183
- Secondary accessions
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Single-pass membrane protein
Note: The ER localization is dependent on the presence of the putative transmembrane helix.
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 1-21 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000310728 | 1-506 | Tabersonine 16-hydroxylase 1 | ||
Expression
Tissue specificity
Predominantly expressed in young leaves of mature plants (PubMed:12228585, PubMed:16262708).
Low expression in roots and flowers, but not detected in stems and old leaves (PubMed:12228585, PubMed:16262708).
Found predominantly in leaf epidermis (PubMed:12228585, PubMed:16262708).
Barely detected in roots, internodes, young and mature leaves, and flower buds, but relatively abundant in fully developed flowers (PubMed:24108213).
Not detected in leaf epidermal cells (PubMed:24108213).
Low expression in roots and flowers, but not detected in stems and old leaves (PubMed:12228585, PubMed:16262708).
Found predominantly in leaf epidermis (PubMed:12228585, PubMed:16262708).
Barely detected in roots, internodes, young and mature leaves, and flower buds, but relatively abundant in fully developed flowers (PubMed:24108213).
Not detected in leaf epidermal cells (PubMed:24108213).
Induction
Up-regulated by methyl jasmonate (PubMed:24108213).
Inhibited by cytochrome c, CO, clotrimazole, miconazole, tricliphane, flusilazole and tetcyclasis, and with a lower efficiency, by 1-phenylimidazole and piperonylbutoxide. Not inhibited by potassium cyanide and sodium azide
Inhibited by cytochrome c, CO, clotrimazole, miconazole, tricliphane, flusilazole and tetcyclasis, and with a lower efficiency, by 1-phenylimidazole and piperonylbutoxide. Not inhibited by potassium cyanide and sodium azide
Developmental stage
Peak of expression after light treatment at 9 days of seedling development.
Structure
Sequence
- Sequence statusComplete
- Length506
- Mass (Da)58,079
- Last updated2017-05-10 v2
- Checksum485FF14E6D34901B
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
FJ647194 EMBL· GenBank· DDBJ | ACM92061.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ238612 EMBL· GenBank· DDBJ | CAB56503.1 EMBL· GenBank· DDBJ | mRNA |