P97849 · S27A1_RAT
- ProteinLong-chain fatty acid transport protein 1
- GeneSlc27a1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids646 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Mediates the import of long-chain fatty acids (LCFA) into the cell by facilitating their transport at the plasma membrane (PubMed:19380575).
Also functions as an acyl-CoA ligase catalyzing the ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates, which prevents fatty acid efflux from cells and might drive more fatty acid uptake. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism. Probably involved in fatty acid transport across the blood barrier (By similarity).
Also functions as an acyl-CoA ligase catalyzing the ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates, which prevents fatty acid efflux from cells and might drive more fatty acid uptake. May act directly as a bona fide transporter, or alternatively, in a cytoplasmic or membrane-associated multimeric protein complex to trap and draw fatty acids towards accumulation. Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis. May be involved in regulation of cholesterol metabolism. Probably involved in fatty acid transport across the blood barrier (By similarity).
Catalytic activity
- a fatty acid(in) = a fatty acid(out)
- (9Z)-octadecenoate(out) = (9Z)-octadecenoate(in)
- hexadecanoate(out) = hexadecanoate(in)
- (5Z,8Z,11Z,14Z)-eicosatetraenoate(out) = (5Z,8Z,11Z,14Z)-eicosatetraenoate(in)
- (9Z,12Z)-octadecadienoate(out) = (9Z,12Z)-octadecadienoate(in)
- a very long-chain fatty acid + ATP + CoA = a very long-chain fatty acyl-CoA + AMP + diphosphateThis reaction proceeds in the forward direction.
- ATP + CoA + tetracosanoate = AMP + diphosphate + tetracosanoyl-CoAThis reaction proceeds in the forward direction.
Activity regulation
Inhibited by Triacsin C.
Features
Showing features for binding site.
GO annotations
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLong-chain fatty acid transport protein 1
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionP97849
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Single-pass membrane protein
Endomembrane system ; Single-pass membrane protein
Note: Plasma membrane and intracellular membranes, at least in adipocytes. In adipocytes, but not myocytes, insulin via the mTORC1 signaling pathway induces a rapid translocation of SLC27A1 from intracellular compartments to the plasma membrane, paralleled by increased LCFA uptake. Insulin-dependent translocation from the cytoplasm to the cell membrane is regulated by EPRS1. Predominantly cytoplasmic in myocytes.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-13 | Extracellular | ||||
Sequence: MRTPGAGTASVAS | ||||||
Transmembrane | 14-34 | Helical | ||||
Sequence: LGLLWLLGLPWTWSAAAAFGV | ||||||
Topological domain | 35-646 | Cytoplasmic | ||||
Sequence: YVGSGGWRFLRIVCKTARRDLFGLSVLIRVRLELRRHRRAGDTIPRIFQAVAQRQPERLALVDASSGICWTFAQLDTYSNAVANLFLQLGFAPGDVVAVFLEGRPEFVGLWLGLAKAGVVAALLNVNLRREPLAFCLGTSAAKALIYGGEMAAAVAEVSEQLGKSLLKFCSGDLGPESVLPDTQLLDPMLAEAPTTPLAQAPGKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRIAAFGHHSYSMRANDVLYDCLPLYHSAGNIMGVGQCIIYGLTVVLRKKFSASRFWDDCVKYNCTVVQYIGEICRYLLRQPVRDVERRHRVRLAVGNGLRPAIWEEFTQGFGVRQIGEFYGATECNCSIANMDGKVGSCGFNSRILTHVYPIRLVKVNEDTMEPLRDSQGLCIPCQPGEPGLLVGQINQQDPLRRFDGYVSDSATNKKIAHSVFRKGDSAYLSGDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEAVLSRLLGQTDVAVYGVAVPGVEGKSGMAAIADPHNQLDPNSMYQELQKVLASYAQPIFLRLLPQVDTTGTFKIQKTRLQREGFDPRQTSDRLFFLDLKQGRYLPLDERVHARICAGDFSL |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000193203 | 1-646 | Long-chain fatty acid transport protein 1 | |||
Sequence: MRTPGAGTASVASLGLLWLLGLPWTWSAAAAFGVYVGSGGWRFLRIVCKTARRDLFGLSVLIRVRLELRRHRRAGDTIPRIFQAVAQRQPERLALVDASSGICWTFAQLDTYSNAVANLFLQLGFAPGDVVAVFLEGRPEFVGLWLGLAKAGVVAALLNVNLRREPLAFCLGTSAAKALIYGGEMAAAVAEVSEQLGKSLLKFCSGDLGPESVLPDTQLLDPMLAEAPTTPLAQAPGKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRIAAFGHHSYSMRANDVLYDCLPLYHSAGNIMGVGQCIIYGLTVVLRKKFSASRFWDDCVKYNCTVVQYIGEICRYLLRQPVRDVERRHRVRLAVGNGLRPAIWEEFTQGFGVRQIGEFYGATECNCSIANMDGKVGSCGFNSRILTHVYPIRLVKVNEDTMEPLRDSQGLCIPCQPGEPGLLVGQINQQDPLRRFDGYVSDSATNKKIAHSVFRKGDSAYLSGDVLVMDELGYMYFRDRSGDTFRWRGENVSTTEVEAVLSRLLGQTDVAVYGVAVPGVEGKSGMAAIADPHNQLDPNSMYQELQKVLASYAQPIFLRLLPQVDTTGTFKIQKTRLQREGFDPRQTSDRLFFLDLKQGRYLPLDERVHARICAGDFSL |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in muscle.
Interaction
Subunit
Self-associates. May function as a homodimer. Interacts with EPRS1; mediates the translocation of SLC27A1 from the cytoplasm to the plasma membrane thereby increasing the uptake of long-chain fatty acids (By similarity).
Interacts with DGAT2 and this interaction is enhanced in the presence of ZFYVE1 (By similarity).
Interacts with DGAT2 and this interaction is enhanced in the presence of ZFYVE1 (By similarity).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 191-475 | Sufficient for oligomerization | ||||
Sequence: EVSEQLGKSLLKFCSGDLGPESVLPDTQLLDPMLAEAPTTPLAQAPGKGMDDRLFYIYTSGTTGLPKAAIVVHSRYYRIAAFGHHSYSMRANDVLYDCLPLYHSAGNIMGVGQCIIYGLTVVLRKKFSASRFWDDCVKYNCTVVQYIGEICRYLLRQPVRDVERRHRVRLAVGNGLRPAIWEEFTQGFGVRQIGEFYGATECNCSIANMDGKVGSCGFNSRILTHVYPIRLVKVNEDTMEPLRDSQGLCIPCQPGEPGLLVGQINQQDPLRRFDGYVSDSATNKK |
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length646
- Mass (Da)71,283
- Last updated1997-05-01 v1
- ChecksumC450CF174CC2EB87
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I5YCE2 | A0A8I5YCE2_RAT | Slc27a1 | 649 | ||
Q6GMM8 | Q6GMM8_RAT | Slc27a1 | 646 | ||
A0A8I6G8J5 | A0A8I6G8J5_RAT | Slc27a1 | 617 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U89529 EMBL· GenBank· DDBJ | AAC53424.1 EMBL· GenBank· DDBJ | mRNA |