P97720 · C11B1_MESAU
- ProteinCytochrome P450 11B1, mitochondrial
- GeneCYP11B1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids499 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids (By similarity).
Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones (By similarity).
Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone (By similarity).
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Due to its lack of 18-oxidation activity, it is incapable of generating aldosterone. Could also be involved in the androgen metabolic pathway (By similarity).
Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones (By similarity).
Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone (By similarity).
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Due to its lack of 18-oxidation activity, it is incapable of generating aldosterone. Could also be involved in the androgen metabolic pathway (By similarity).
Catalytic activity
- a steroid + 2 H+ + O2 + 2 reduced [adrenodoxin] = an 11beta-hydroxysteroid + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = cortisol + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = corticosterone + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxy-11-deoxycorticosterone + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = 19-hydroxy-11-deoxycorticosterone + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- cortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxycortisol + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
- 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxy-11-deoxycortisol + H2O + 2 oxidized [adrenodoxin]This reaction proceeds in the forward direction.
Cofactor
Pathway
Steroid biosynthesis; glucocorticoid biosynthesis.
Steroid hormone biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | mitochondrial inner membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | steroid 11-beta-monooxygenase activity | |
Biological Process | glucocorticoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 11B1, mitochondrial
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Cricetidae > Cricetinae > Mesocricetus
Accessions
- Primary accessionP97720
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Mitochondrion inner membrane ; Peripheral membrane protein
Keywords
- Cellular component
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-24 | Mitochondrion | ||||
Sequence: MALRAKADVWLARPWQCLPRTRAL | ||||||
Chain | PRO_0000003598 | 25-499 | Cytochrome P450 11B1, mitochondrial | |||
Sequence: GTTAALAPNTLRPFEAIPQYSRNRWLKMLQILREEGQEGLHLEMHEAFRELGPIFRYSMGRTQVVYVMLPEVAEKVFQADSTQPSRTLLEPWVAHREHRGLSRGVFLLNGPEWRFNRLRINPHMLSPKAVQKFVPMVDMVARDFLEFLKKKVLANAHGSLSMNFYSSMFNYTIEASHFVLFGERLGLLGDDLNSGSLKFVNALNSIMKTTPQLMLLPSGLTRWISTRVWKENFDSWDFVSEYVTKNVKNVYQEVQSGGPQSWSVISQLVAEGALTMDAILANSLELTAGSVDTTSVPLVMTLFELARNPDVQQAVRQESLAAEASVAANPQRAMSDLPLLRAVLKETLRLYPVAVFLERILSSDLVLQNYHVPAGTILHMSLYSMGRNPAVFPRPEHYLPQRWLERNGSFQHLTFGFGVRQCLGKRLAQVEMLLLLHHVLKSFRVETQEREDVRMVYRFVLAPSSSPLLTFRPVS |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length499
- Mass (Da)56,821
- Last updated1997-05-01 v1
- ChecksumC798467266D741BD
Keywords
- Technical term