P97720 · C11B1_MESAU

Function

function

A cytochrome P450 monooxygenase involved in the biosynthesis of adrenal corticoids (By similarity).
Catalyzes a variety of reactions that are essential for many species, including detoxification, defense, and the formation of endogenous chemicals like steroid hormones (By similarity).
Steroid 11beta, 18- and 19-hydroxylase with preferred regioselectivity at 11beta, then 18, and lastly 19. Catalyzes the hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone (21-hydroxyprogesterone) at 11beta position, yielding cortisol or corticosterone, respectively, but cannot produce aldosterone (By similarity).
Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate for hydroxylation and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). Due to its lack of 18-oxidation activity, it is incapable of generating aldosterone. Could also be involved in the androgen metabolic pathway (By similarity).

Catalytic activity

  • a steroid + 2 H+ + O2 + 2 reduced [adrenodoxin] = an 11beta-hydroxysteroid + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
    EC:1.14.15.4 (UniProtKB | ENZYME | Rhea)
  • 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = cortisol + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
  • 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = corticosterone + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
  • 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxy-11-deoxycorticosterone + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
  • 21-hydroxyprogesterone + 2 H+ + O2 + 2 reduced [adrenodoxin] = 19-hydroxy-11-deoxycorticosterone + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
  • cortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxycortisol + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.
  • 11-deoxycortisol + 2 H+ + O2 + 2 reduced [adrenodoxin] = 18-hydroxy-11-deoxycortisol + H2O + 2 oxidized [adrenodoxin]
    This reaction proceeds in the forward direction.

Cofactor

heme (UniProtKB | Rhea| CHEBI:30413 )

Pathway

Steroid biosynthesis; glucocorticoid biosynthesis.
Steroid hormone biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site446Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue

GO annotations

AspectTerm
Cellular Componentmitochondrial inner membrane
Molecular Functionheme binding
Molecular Functioniron ion binding
Molecular Functionsteroid 11-beta-monooxygenase activity
Biological Processglucocorticoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Cytochrome P450 11B1, mitochondrial
  • Alternative names
    • CYPXIB1
    • Cytochrome P450C11
    • Steroid 11-beta-hydroxylase, CYP11B1
      (EC:1.14.15.4
      ) . EC:1.14.15.4 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      CYP11B1

Organism names

  • Taxonomic identifier
  • Strain
    • Syrian
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Cricetidae > Cricetinae > Mesocricetus

Accessions

  • Primary accession
    P97720

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for transit peptide, chain.

TypeIDPosition(s)Description
Transit peptide1-24Mitochondrion
ChainPRO_000000359825-499Cytochrome P450 11B1, mitochondrial

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the cytochrome P450 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    499
  • Mass (Da)
    56,821
  • Last updated
    1997-05-01 v1
  • Checksum
    C798467266D741BD
MALRAKADVWLARPWQCLPRTRALGTTAALAPNTLRPFEAIPQYSRNRWLKMLQILREEGQEGLHLEMHEAFRELGPIFRYSMGRTQVVYVMLPEVAEKVFQADSTQPSRTLLEPWVAHREHRGLSRGVFLLNGPEWRFNRLRINPHMLSPKAVQKFVPMVDMVARDFLEFLKKKVLANAHGSLSMNFYSSMFNYTIEASHFVLFGERLGLLGDDLNSGSLKFVNALNSIMKTTPQLMLLPSGLTRWISTRVWKENFDSWDFVSEYVTKNVKNVYQEVQSGGPQSWSVISQLVAEGALTMDAILANSLELTAGSVDTTSVPLVMTLFELARNPDVQQAVRQESLAAEASVAANPQRAMSDLPLLRAVLKETLRLYPVAVFLERILSSDLVLQNYHVPAGTILHMSLYSMGRNPAVFPRPEHYLPQRWLERNGSFQHLTFGFGVRQCLGKRLAQVEMLLLLHHVLKSFRVETQEREDVRMVYRFVLAPSSSPLLTFRPVS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
S82228
EMBL· GenBank· DDBJ
AAB47144.1
EMBL· GenBank· DDBJ
mRNA

Similar Proteins

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