P97369 · NCF4_MOUSE

  • Protein
    Neutrophil cytosol factor 4
  • Gene
    Ncf4
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.

Features

Showing features for binding site.

133950100150200250300
TypeIDPosition(s)Description
Binding site58-60a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) (UniProtKB | ChEBI)
Binding site92-94a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytosol
Cellular Componentendosome membrane
Cellular Componentmembrane
Cellular ComponentNADPH oxidase complex
Molecular Functionphosphatidylinositol-3-phosphate binding
Molecular Functionsuperoxide-generating NADPH oxidase activator activity
Biological Processphagocytosis
Biological Processrespiratory burst
Biological Processsuperoxide anion generation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Neutrophil cytosol factor 4
  • Short names
    NCF-4
  • Alternative names
    • Neutrophil NADPH oxidase factor 4
    • p40-phox (p40phox)

Gene names

    • Name
      Ncf4

Organism names

  • Taxonomic identifier
  • Strains
    • C57BL/6J
    • FVB/N
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    P97369
  • Secondary accessions
    • Q3TBC6

Proteomes

Organism-specific databases

Subcellular Location

Cytoplasm, cytosol
Endosome membrane
; Peripheral membrane protein
Membrane
; Peripheral membrane protein

Keywords

PTM/Processing

Features

Showing features for chain, modified residue.

TypeIDPosition(s)Description
ChainPRO_00000967651-339Neutrophil cytosol factor 4
Modified residue154Phosphothreonine
Modified residue315Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4 (By similarity).
Interacts with NCF2 (PubMed:21551061).
Interacts wirh NCF1 (By similarity).
The NCF2-NCF4 complex interacts with GBP7 (via GB1/RHD3-type G domain) (PubMed:21551061).

Protein-protein interaction databases

Miscellaneous

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain19-140PX
Domain170-229SH3
Domain237-329PB1

Domain

The PB1 domain mediates the association with NCF2/p67-PHOX.
The PX domain mediates interaction with membranes enriched in phosphatidylnositol 3-phosphate.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    339
  • Mass (Da)
    38,707
  • Last updated
    2011-07-27 v2
  • Checksum
    62C4B8FC2D2C4CB1
MALAQQLRSESDFEQLPDDVAVSANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFYALQSKLEERFGPESKNSPFTCSLPTLPAKVYMGAKQEIAETRIPALNAYMKNLLSLPVCVLMDPDVRIFFYQSAYDAEQVPQALRRLRPRTRKIKGVSPQGAIMDRMEAPRAEALFDFTGNSKLELSFKAGDVIFLLSKINKDWLEGTSQGATGIFPGSFVKILKDFPEDEDTTNWLRCYFYEDTGKTIKDIAVEEDLSSTPLFKDLLALMRREFQREDIALSYQDAEGDLVRLLSDEDVGLMVKQARGLPSQKRLFPWKLHVTQKDNYSVYNTVP

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A8XU21A8XU21_MOUSENcf4252

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict85in Ref. 1; AAC53122, 2; BAA25651 and 6; AAH25517

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U59488
EMBL· GenBank· DDBJ
AAC53122.1
EMBL· GenBank· DDBJ
mRNA
AB002665
EMBL· GenBank· DDBJ
BAA25651.1
EMBL· GenBank· DDBJ
mRNA
AK150510
EMBL· GenBank· DDBJ
BAE29623.1
EMBL· GenBank· DDBJ
mRNA
AK171315
EMBL· GenBank· DDBJ
BAE42387.1
EMBL· GenBank· DDBJ
mRNA
AL589692
EMBL· GenBank· DDBJ
-Genomic DNA No translation available.
CH466545
EMBL· GenBank· DDBJ
EDL29666.1
EMBL· GenBank· DDBJ
Genomic DNA
BC025517
EMBL· GenBank· DDBJ
AAH25517.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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