P96192 · DUSC_AZOVI

  • Protein
    tRNA-dihydrouridine(16) synthase
  • Gene
    dusC
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site, site, active site.

130920406080100120140160180200220240260280300
TypeIDPosition(s)Description
Binding site7-9FMN (UniProtKB | ChEBI)
Site35Interacts with tRNA; defines subfamily-specific binding signature
Binding site68FMN (UniProtKB | ChEBI)
Site95Interacts with tRNA
Active site98Proton donor
Binding site137FMN (UniProtKB | ChEBI)
Site175Interacts with tRNA
Binding site198FMN (UniProtKB | ChEBI)
Binding site220-221FMN (UniProtKB | ChEBI)
Site267Interacts with tRNA; defines subfamily-specific binding signature
Site286Interacts with tRNA; defines subfamily-specific binding signature

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA-dihydrouridine16 synthase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-dihydrouridine(16) synthase
  • EC number
  • Alternative names
    • U16-specific dihydrouridine synthase
      (U16-specific Dus
      )
    • tRNA-dihydrouridine synthase C

Gene names

    • Name
      dusC

Organism names

  • Taxonomic identifier
  • Strain
    • ATCC 13705 / OP1 / DSM 366 / NCIMB 11614 / LMG 3878 / UW
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Azotobacter

Accessions

  • Primary accession
    P96192

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001621081-309tRNA-dihydrouridine16 synthase

Structure

Family & Domains

Sequence similarities

Belongs to the Dus family. DusC subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    309
  • Mass (Da)
    33,181
  • Last updated
    1997-05-01 v1
  • Checksum
    2D40B965C6DF551B
MRIALAPMEGLVDELLRDLLTRVGGIDWCVTEFVRVCDRLLPVAQFEKLAPELRHGWRTRAGTPMHLQLLGSDPACLAENAALAAELGAPAIDLNFGCPAKTSTARGADGCCSTSRNCCMPSSARCAGPCRRCAGDRQDAPGVRPARGRPGVRRALVEGGVAHLVVHARTKVEGYRPPASWEWLARVREAVAVPVYANRKSGRRRIGCREISGVEDVMLGCGLVSRPDLARQIAMRAPDARSSRRAGVRYSRWCASSGGAPGSGSRRATPLAGSSSGWACWRAVIRRRPRCSPNCAGRTTAGGWMPCWA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Y11280
EMBL· GenBank· DDBJ
CAA72146.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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