P96192 · DUSC_AZOVI
- ProteintRNA-dihydrouridine(16) synthase
- GenedusC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids309 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U16 in tRNAs.
Catalytic activity
- 5,6-dihydrouridine16 in tRNA + NADP+ = H+ + NADPH + uridine16 in tRNA
- 5,6-dihydrouridine16 in tRNA + NAD+ = H+ + NADH + uridine16 in tRNA
Cofactor
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 7-9 | FMN (UniProtKB | ChEBI) | ||||
Sequence: PME | ||||||
Site | 35 | Interacts with tRNA; defines subfamily-specific binding signature | ||||
Sequence: R | ||||||
Binding site | 68 | FMN (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Site | 95 | Interacts with tRNA | ||||
Sequence: N | ||||||
Active site | 98 | Proton donor | ||||
Sequence: C | ||||||
Binding site | 137 | FMN (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Site | 175 | Interacts with tRNA | ||||
Sequence: Y | ||||||
Binding site | 198 | FMN (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 220-221 | FMN (UniProtKB | ChEBI) | ||||
Sequence: GC | ||||||
Site | 267 | Interacts with tRNA; defines subfamily-specific binding signature | ||||
Sequence: R | ||||||
Site | 286 | Interacts with tRNA; defines subfamily-specific binding signature | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-dihydrouridine16 synthase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-dihydrouridine(16) synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Azotobacter
Accessions
- Primary accessionP96192
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000162108 | 1-309 | tRNA-dihydrouridine16 synthase | |||
Sequence: MRIALAPMEGLVDELLRDLLTRVGGIDWCVTEFVRVCDRLLPVAQFEKLAPELRHGWRTRAGTPMHLQLLGSDPACLAENAALAAELGAPAIDLNFGCPAKTSTARGADGCCSTSRNCCMPSSARCAGPCRRCAGDRQDAPGVRPARGRPGVRRALVEGGVAHLVVHARTKVEGYRPPASWEWLARVREAVAVPVYANRKSGRRRIGCREISGVEDVMLGCGLVSRPDLARQIAMRAPDARSSRRAGVRYSRWCASSGGAPGSGSRRATPLAGSSSGWACWRAVIRRRPRCSPNCAGRTTAGGWMPCWA |
Structure
Sequence
- Sequence statusComplete
- Length309
- Mass (Da)33,181
- Last updated1997-05-01 v1
- Checksum2D40B965C6DF551B