P96134 · OTC_THET2
- ProteinOrnithine carbamoyltransferase
- GeneargF
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids301 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline, which is a substrate for argininosuccinate synthetase, the enzyme involved in the final step in arginine biosynthesis.
Catalytic activity
- carbamoyl phosphate + L-ornithine = H+ + L-citrulline + phosphate
Activity regulation
Inhibited by delta-N-phosphonoacetyl-L-ornithine.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.1 mM | carbamoyl phosphate | 7 | ||||
0.1 mM | L-ornithine | 7 |
pH Dependence
Optimum pH is dependent on ornithine concentration with an optimum at pH 8 for ornithine concentrations around KM values. Higher concentrations shift the optimum towards lower pH. At pH 7.0, the maximum velocity remains constant even when the ornithine concentration is very high (> 10 mM). At more alkaline pH, however, substrate inhibition is observed above 0.25 mM ornithine.
Temperature Dependence
Optimum temperature is 65 degrees Celsius. Highly thermostable.
Pathway
Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 107 | carbamoyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 134-137 | carbamoyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: HPLQ | ||||||
Binding site | 165 | L-ornithine (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 220 | L-ornithine (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 224-225 | L-ornithine (UniProtKB | ChEBI) | ||||
Sequence: SM | ||||||
Binding site | 260-261 | carbamoyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: CL | ||||||
Binding site | 288 | carbamoyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | amino acid binding | |
Molecular Function | ornithine carbamoyltransferase activity | |
Biological Process | arginine biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameOrnithine carbamoyltransferase
- EC number
- Short namesOTC ; OTCase
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Deinococcota > Deinococci > Thermales > Thermaceae > Thermus
Accessions
- Primary accessionP96134
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000113050 | 1-301 | Ornithine carbamoyltransferase | |||
Sequence: MGGEALTLPKDLLDFSGYGPKELQALLDLAERLKRERYRGEDLKGKVLALLFEKPSLRTRTTLEVAMVHLGGHAVYLDQKQVGIGEREPVRDVAKNLERFVEGIAARVFRHETVEALARHAKVPVVNALSDRAHPLQALADLLTLKEVFGGLAGLEVAWVGDGNNVLNSLLEVAPLAGLKVRVATPKGYEPDPGLLKRANAFFTHDPKEAALGAHALYTDVWTSMGQEAERAKRLRDFQGFQVNGELLKLLRPEGVFLHCLPAHYGEETTEEAVHGPRSRVFDQAENRLHTAKAVLLTLLK |
Interaction
Subunit
Homotrimer.
Structure
Sequence
- Sequence statusComplete
- Length301
- Mass (Da)33,205
- Last updated1997-05-01 v1
- Checksum0433F585C7E9DD23
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Y10266 EMBL· GenBank· DDBJ | CAA71315.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
Y18353 EMBL· GenBank· DDBJ | CAA77140.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AE017221 EMBL· GenBank· DDBJ | AAS81184.1 EMBL· GenBank· DDBJ | Genomic DNA |